Molecular cloning and functional expression of mannitol-1-phosphatase from the apicomplexan parasite Eimeria tenella

• Published in: The Journal of biological chemistry Vol. 273; no. 7; pp. 4237 - 4244
• Main Authors: Liberator, P, Anderson, J, Feiglin, M, Sardana, M, Griffin, P, Schmatz, D, Myers, R.W
• Format: Journal Article
• Language: English
• Published: United States American Society for Biochemistry and Molecular Biology 13.02.1998
• Subjects: ACTIVIDAD ENZIMATICA; ACTIVITE ENZYMATIQUE; ADN; Amino Acid Sequence; AMINO ACID SEQUENCES; analysis; Animals; Base Sequence; Binding Sites; CHEMICAL COMPOSITION; chemistry; CLONACION; CLONAGE; CLONING; Cloning, Molecular; COMPLEMENTARY DNA; COMPOSICION QUIMICA; COMPOSITION CHIMIQUE; DIETHYL PYROCARBONATE; Diethyl Pyrocarbonate - pharmacology; DNA; EIMERIA TENELLA; Eimeria tenella - enzymology; enzyme activity; ENZYME INHIBITORS; ENZYMIC ACTIVITY; enzymology; ESTERASAS; ESTERASE; ESTERASES; GENBANK/AF032462; Gene Expression; Gene Expression - genetics; genetics; HISTIDINA; HISTIDINE; INHIBICION; INHIBIDORES DE ENZIMAS; INHIBITEUR D'ENZYME; INHIBITION; Kinetics; metabolism; MOLECULAR SEQUENCE DATA; NUCLEOTIDE SEQUENCE; nucleotide sequences; Peptide Fragments; Peptide Fragments - analysis; pharmacology; PHOSPHORIC MONOESTER HYDROLASES; Phosphoric Monoester Hydrolases - chemistry; Recombinant Proteins; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; SECUENCIA NUCLEOTIDICA; Sequence Analysis, DNA; Sequence Homology, Amino Acid; SEQUENCE NUCLEOTIDIQUE; Trypsin; Trypsin - metabolism

A metabolic pathway responsible for the biosynthesis and utilization of mannitol is present in the seven species of Eimeria that infect chickens, but is not in the avian host. Mannitol-1-phosphatase (M1Pase), a key enzyme for mannitol biosynthesis, is a highly substrate-specific phosphatase and, acc...