Electrochemical sensing of the thyroid hormone thyronamine (T0AM) via molecular imprinted polymers (MIPs)

• Published in: Talanta (Oxford) Vol. 194; pp. 689 - 696
• Main Authors: Pacheco, João G., Rebelo, Patrícia, Cagide, Fernando, Gonçalves, Luís Moreira, Borges, Fernanda, Rodrigues, José António, Delerue-Matos, Cristina
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.03.2019
• Subjects: Electroanalysis; Electropolymerization; Endocrinology; Modified working electrode; Molecular recognition; Poly(para-aminobenzoic acid) film; Molecular recognition; Electroanalysis; Modified working electrode; Poly(para-aminobenzoic acid) film; Electropolymerization; Endocrinology
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2018.10.090

Recent studies have shown that besides the well-known T3 (triiodothyronine) and T4 (thyroxine) there might be other important thyroid hormones, in particular T0AM (thyronamine) and T1AM (3-iodothyronamine). The absence of a large number of studies showing their precise importance might be explained by the limited number of analytical methodologies available. This work aims to show an electroanalytical alternative making use of electropolymerized molecularly imprinted polymer (MIPs). The MIPs' polymerization is performed on the surface of screen-printed carbon electrodes (SPCEs), using 4–aminobenzoic acid (4-ABA) as the building and functional monomer and the analyte T0AM as the template. The step-by-step construction of the SPCE-MIP sensor was studied by cyclic voltammetry (CV) and by electrochemical impedance spectroscopy (EIS). After optimization, by means of square-wave voltammetry, the SPCE-MIP showed suitable selectivity (in comparison with other thyroid hormones and catechol amines), repeatability (intra-day of 3.9%), a linear range up to 10 μmol L−1 (0.23 × 103 μg dL−1) with an r2 of 0.998 and a limit of detection (LOD) and quantification (LOQ) of 0.081 and 0.27 μmol L−1 (1.9 and 6.2 μg dL−1), respectively. [Display omitted] •First time a sensor was developed for T0AM (thyronamine);•Electroanalytical methodology for T0AM;•Molecularly imprinted polymers (MIPs) were developed for T0AM;•MIPs' polymerization (4–aminobenzoic acid to a poly(4-aminobenzoic acid) film) occurred in the surface of screen-printed carbon electrodes (SPCEs);•The SPCE-MIP selectively analyzed T0AM by square-wave voltammetry (SWV).