Novel glycosylated human interferon alpha 2b expressed in glycoengineered Pichia pastoris and its biological activity: N-linked glycoengineering approach

• Published in: Enzyme and microbial technology Vol. 128; pp. 49 - 58
• Main Authors: Katla, Srikanth, Yoganand, K.N.R., Hingane, Smita, Ranjith Kumar, C.T., Anand, B., Sivaprakasam, Senthilkumar
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.09.2019
• Subjects: adverse effects; cell lines; Escherichia coli; Glycoengineering; glycosylation; half life; humans; immunomodulators; Interferon alpha 2b; interferons; Komagataella pastoris; laboratory animals; markets; mass spectrometry; moieties; N-glycosylation; P. pastoris; pharmacokinetics; product quality; rats; virus replication; Interferon alpha 2b; P. pastoris; N-glycosylation; Glycoengineering

[Display omitted] •N-glycoengineering approach for the design of novel glycosylated human IFN α2b.•Successful human-like N-glycosylated IFN α2b expression by glycoengineered P. pastoris.•Improved plasma half-life of purified glycosylated human IFN α2b.•Glycosylated human IFN α2b is biologically acti...