In situ analysis of tyrosine phosphorylation networks by FLIM on cell arrays

• Published in: Nature methods Vol. 7; no. 6; pp. 467 - 472
• Main Authors: Grecco, Hernán E, Frahm, Thomas, Hou, Jian, Truxius, Dina C, Bastiaens, Philippe I H, Roda-Navarro, Pedro, Squire, Anthony, Girod, Andreas, Pepperkok, Rainer
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.06.2010; Nature Publishing Group
• Subjects: 631/114/2391; 631/1647/245/2225; 631/1647/527/2047; 631/80/458/1733; Bioinformatics; Biological Microscopy; Biological Techniques; Biomedical and Life Sciences; Biomedical Engineering/Biotechnology; Cell Line, Tumor; Cellular biology; Energy transfer; Epidermal Growth Factor - pharmacology; Fluorescence; Fluorescence Resonance Energy Transfer; Humans; Life Sciences; Microscopy; Microscopy, Fluorescence - methods; Phosphoproteins - analysis; Phosphorylation; Physiological aspects; Post-translational modification; Protein Processing, Post-Translational; Protein Tyrosine Phosphatases - metabolism; Protein-Tyrosine Kinases - metabolism; Proteins; Proteomics; Receptor, Epidermal Growth Factor - metabolism; Research methodology; Resonance; Signal transduction; Tyrosine; Tyrosine - metabolism; Greece; Germany; Finland

Combining reverse transfection of protein tyrosine kinase substrates on cell arrays with fluorescence resonance energy transfer (FRET) measurements by fluorescence lifetime imaging microscopy (FLIM) allows quantitative assessment of phosphorylation patterns and identification of feedback loops at si...