Proteomic comparison of different synaptosome preparation procedures

• Published in: Amino acids Vol. 52; no. 11-12; pp. 1529 - 1543
• Main Authors: Gulyássy, Péter, Puska, Gina, Györffy, Balázs A., Todorov-Völgyi, Katalin, Juhász, Gábor, Drahos, László, Kékesi, Katalin Adrienna
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01.12.2020; Springer Nature B.V
• Subjects: Analytical Chemistry; Biochemical Engineering; Biochemistry; Biomedical and Life Sciences; Contamination; Depletion; Electron microscopy; homogenization; isolation techniques; Life Sciences; Liquid chromatography; Mass spectrometry; Mass spectroscopy; Membrane potential; Membrane proteins; Membranes; Mitochondria; nerve tissue; Nervous system; Neurobiology; Neurosciences; Neurotransmission; Neurotransmitters; Organelles; Original; Original Article; Postsynaptic density; Proteins; Proteomics; Qualitative analysis; quantitative analysis; Structure-function relationships; Synapses; Synaptic transmission; Synaptic vesicles; Synaptosomes; variance; Western blotting; Synapse; Synaptosome; Neuroproteomics; Subcellular proteomics; Proteomics

Synaptosomes are frequently used research objects in neurobiology studies focusing on synaptic transmission as they mimic several aspects of the physiological synaptic functions. They contain the whole apparatus for neurotransmission, the presynaptic nerve ending with synaptic vesicles, synaptic mit...