Entamoeba histolytica thioredoxin reductase: Molecular and functional characterization of its atypical properties

• Published in: Biochimica et biophysica acta Vol. 1820; no. 12; pp. 1859 - 1866
• Main Authors: Arias, Diego G., Regner, Erika L., Iglesias, Alberto A., Guerrero, Sergio A.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.12.2012
• Subjects: Antioxidant; Entamoeba; Entamoeba histolytica; Entamoeba histolytica - enzymology; Flavin-Adenine Dinucleotide - metabolism; hydrogen peroxide; Hydrogen Peroxide - metabolism; Kinetics; methylene blue; molecular weight; NAD - metabolism; NAD(P)H Dehydrogenase (Quinone) - metabolism; NADP (coenzyme); NADP - metabolism; nitrogen; Oxidation-Reduction; Oxidative Stress; oxygen; parasites; quinones; Reducing power; S-nitrosothiol; S-Nitrosothiols - chemistry; Thioredoxin; Thioredoxin-Disulfide Reductase - metabolism; Thioredoxins - metabolism; DTBBQ; DTNB; MV; CDNB; GSNO; Entamoeba; Thioredoxin; Antioxidant; Reducing power; MB; CySNO; S-nitrosothiol; TRX; MBQ; TRXR

Entamoeba histolytica, an intestinal protozoan that is the causative agent of amoebiasis, is exposed to elevated amounts of highly toxic reactive oxygen and nitrogen species during tissue invasion. Thioredoxin reductase catalyzes the reversible transfer of reducing equivalents between NADPH and thio...