Human chromosome segregation involves multi-layered regulation of separase by the peptidyl-prolyl-isomerase Pin1

• Published in: Molecular cell Vol. 58; no. 3; pp. 495 - 506
• Main Authors: Hellmuth, Susanne, Rata, Scott, Brown, Andreas, Heidmann, Stefan, Novak, Bela, Stemmann, Olaf
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 07.05.2015
• Subjects: anaphase; Anaphase - genetics; CDC2 Protein Kinase; chromatids; Chromatids - genetics; chromatin; chromosome segregation; Chromosome Segregation - genetics; Cyclin B1 - chemistry; Cyclin B1 - genetics; Cyclin B1 - metabolism; Cyclin-Dependent Kinases - chemistry; Cyclin-Dependent Kinases - genetics; Cyclin-Dependent Kinases - metabolism; cyclins; enzyme inhibition; Gene Expression Regulation, Enzymologic; half life; HEK293 Cells; Humans; Immunoblotting; isomerases; isomerization; Metaphase - genetics; mice; Microscopy, Fluorescence; mitosis; Mitosis - genetics; Models, Genetic; Models, Molecular; Mutation; NIMA-Interacting Peptidylprolyl Isomerase; Peptidylprolyl Isomerase - genetics; Peptidylprolyl Isomerase - metabolism; phosphorylation; Protein Binding; Protein Conformation; proteolysis; RNA Interference; Securin - genetics; Securin - metabolism; separase; Separase - chemistry; Separase - genetics; Separase - metabolism; telophase; ubiquitin

Ring shaped cohesin keeps sister chromatids paired until cleavage of its Scc1/Rad21 subunit by separase triggers chromosome segregation in anaphase. Vertebrate separase is held inactive by mutually exclusive binding to securin or Cdk1-cyclin B1 and becomes unleashed only upon ubiquitin dependent deg...