Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization Assays - Crawford, Katharine H. D.; Eguia, Rachel; Dingens, Adam S.; Loes, Andrea N.; Malone, Keara D.; Wolf, Caitlin R.; Chu, Helen Y.; Tortorici, M. Alejandra; Veesler, David; Murphy, Michael; Pettie, Deleah; King, Neil P.; et al. (Viruses, 2020)
Keywords: Angiotensin-Converting Enzyme 2; Antibodies, Neutralizing; Antibodies, Neutralizing - immunology; Antibodies, Viral; Antibodies, Viral - blood; Antibodies, Viral - immunology; Biochemical assays; Containment of Biohazards; coronavirus; Coronaviruses; COVID-19; Diagnosis; Health aspects; HEK293 Cells; Humans; lentiviral pseudotype; Lentivirus; Life Sciences; Methods; Microbiology and Parasitology; neutralization assay; Neutralization Tests; Neutralization Tests - methods; Peptidyl-Dipeptidase A; Peptidyl-Dipeptidase A - metabolism; Plasma; Plasma - immunology; Protocol; SARS-CoV-2; Spike; Spike Glycoprotein, Coronavirus; Spike Glycoprotein, Coronavirus - analysis; Virology; United States; COVID-19; Spike; ACE2; SARS-CoV-2; cytoplasmic tail; ALAYT; lentiviral pseudotype; luciferase; coronavirus; neutralization assay; 293T-ACE2