Development and evaluation of silver amplification immunochromatography kit for foot-and-mouth disease virus antigen detection

• Published in: Journal of Virological Methods Vol. 275; p. 113736
• Main Authors: Morioka, Kazuki, Urayama, Kana, Wada, Atsuhiko, Yoshida, Kazuo, Kato, Tomoko, Kitano, Rie, Nishi, Tatsuya, Kanno, Toru, Yamada, Manabu, Yamakawa, Makoto, Ulziibat, Gelermaa, Makino, Yoshihiko, Nakamura, Kentaro, Hojo, Eri, Matsumoto, Takashi, Fukai, Katsuhiko
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.01.2020; Elsevier BV
• Subjects: Animals; Antigen detection; Antigens, Viral; Antigens, Viral - isolation & purification; Cattle; Cattle Diseases; Cattle Diseases - diagnosis; Cell Line; Chromatography, Affinity; Chromatography, Affinity - instrumentation; Foot-and-Mouth Disease; Foot-and-Mouth Disease - diagnosis; Foot-and-Mouth Disease Virus; Foot-and-Mouth Disease Virus - classification; Foot-and-Mouth Disease Virus - isolation & purification; Immunochromatography; Reagent Kits, Diagnostic; Sensitivity and Specificity; Serogroup; Silver; Silver - chemistry; Silver amplification; Antigen detection; Foot-and-mouth disease virus; Immunochromatography; Silver amplification

•A silver amplification immunochromatography was developed for FMDV antigen detection.•FMDV-Ag SAI increased the sensitivity of conventional immunochromatographic assay.•FMDV-Ag SAI was able to detect specific antigen even in saliva samples.•FMDV-Ag SAI was evaluated with various clinical samples fr...