Development and evaluation of silver amplification immunochromatography kit for foot-and-mouth disease virus antigen detection

• Published in: Journal of Virological Methods Vol. 275; p. 113736
• Main Authors: Morioka, Kazuki, Urayama, Kana, Wada, Atsuhiko, Yoshida, Kazuo, Kato, Tomoko, Kitano, Rie, Nishi, Tatsuya, Kanno, Toru, Yamada, Manabu, Yamakawa, Makoto, Ulziibat, Gelermaa, Makino, Yoshihiko, Nakamura, Kentaro, Hojo, Eri, Matsumoto, Takashi, Fukai, Katsuhiko
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.01.2020; Elsevier BV
• Subjects: Animals; Antigen detection; Antigens, Viral; Antigens, Viral - isolation & purification; Cattle; Cattle Diseases; Cattle Diseases - diagnosis; Cell Line; Chromatography, Affinity; Chromatography, Affinity - instrumentation; Foot-and-Mouth Disease; Foot-and-Mouth Disease - diagnosis; Foot-and-Mouth Disease Virus; Foot-and-Mouth Disease Virus - classification; Foot-and-Mouth Disease Virus - isolation & purification; Immunochromatography; Reagent Kits, Diagnostic; Sensitivity and Specificity; Serogroup; Silver; Silver - chemistry; Silver amplification; Antigen detection; Foot-and-mouth disease virus; Immunochromatography; Silver amplification
• DOI: 10.1016/j.jviromet.2019.113736
• ISSN: 0166-0934; 1879-0984

•A silver amplification immunochromatography was developed for FMDV antigen detection.•FMDV-Ag SAI increased the sensitivity of conventional immunochromatographic assay.•FMDV-Ag SAI was able to detect specific antigen even in saliva samples.•FMDV-Ag SAI was evaluated with various clinical samples from an outbreak. A silver amplification immunochromatography (SAI) kit for the detection of all seven serotypes of foot-and-mouth disease virus (FMDV)—FMDV-Ag SAI—was developed using the monoclonal antibody 1H5 recognizing the highly conserved N terminus region of VP2. The FMDV-Ag SAI can be used under conditions of high biosecurity containment as it does not require any apparatus. The FMDV-Ag SAI exhibited 10–100 times higher sensitivity against the five serotypes (O, A, Asia1, C, and SAT1) and similar sensitivity against SAT2 and SAT3, compared with the Svanodip® FMDV-Ag kit immunochromatography kit. The Svanodip kit showed inhibitory results with several saliva samples but not with the FMDV-Ag SAI kit. In a validation study using clinical samples (n = 132; vesicular epithelium = 92, vesicular lesion swabs = 20, saliva = 20) in Mongolia, the sensitivity of FMDV-Ag SAI in comparison with real-time reverse transcription-polymerase chain reaction revealed the following data: vesicular epithelium, 85.4% (76/89); vesicular lesion swab, 46.7% (7/17); and saliva, 36.8% (7/19). No cross-reactivity with the non-FMDV vesicular-forming viruses and taxonomically related viruses of the Picornaviridae family occurred. The FMDV-Ag SAI is a highly sensitive diagnostic tool that enables pen-side diagnosis without requiring the use of any equipment.