Inactivation of iron-sulfur cluster biogenesis regulator SufR in Synechocystis sp. PCC 6803 induces unique iron-dependent protein-level responses

• Published in: Biochimica et biophysica acta. General subjects Vol. 1861; no. 5; pp. 1085 - 1098
• Main Authors: Vuorijoki, Linda, Tiwari, Arjun, Kallio, Pauli, Aro, Eva-Mari
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.05.2017
• Subjects: biogenesis; carbon; electron transfer; Electron Transport - physiology; EPR spectroscopy; Gene regulation; iron; Iron - metabolism; Iron-sulfur clusters; Iron-Sulfur Proteins - metabolism; operon; Operon - physiology; photosynthesis; Photosystem I Protein Complex - metabolism; Photosystem II Protein Complex - metabolism; pyruvate synthase; quantitative polymerase chain reaction; repressor proteins; reverse transcriptase polymerase chain reaction; RT-qPCR; SRM proteomics; Sulfur - metabolism; Synechocystis - metabolism; Synechocystis sp. PCC 6714; Synechocystis sp. PCC 6803; SUF; DTT; EPR; Gene regulation; IAA; Iron-sulfur clusters; FeCN; Synechocystis sp. PCC 6803; SRM; SRM proteomics; ROS; Fe-S cluster; RT-qPCR; PSI/II; HPLC; EPR spectroscopy

Iron-sulfur (Fe-S) clusters are protein-bound cofactors associated with cellular electron transport and redox sensing, with multiple specific functions in oxygen-evolving photosynthetic cyanobacteria. The aim here was to elucidate protein-level effects of the transcriptional repressor SufR involved...