Fulcrum: condensing redundant reads from high-throughput sequencing studies

• Published in: Bioinformatics (Oxford, England) Vol. 28; no. 10; pp. 1324 - 1327
• Main Authors: Burriesci, Matthew S., Lehnert, Erik M., Pringle, John R.
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 15.05.2012
• Subjects: Algorithms; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Gene Expression Profiling; General aspects; High-Throughput Nucleotide Sequencing - methods; Humans; Mathematics in biology. Statistical analysis. Models. Metrology. Data processing in biology (general aspects); Original Papers; Pseudomonas - genetics; Sequence Analysis, DNA - methods; Software; High throughput screening; High throughput sequencing
• ISSN: 1367-4803; 1367-4811; 1367-4811
• DOI: 10.1093/bioinformatics/bts123

Motivation: Ultra-high-throughput sequencing produces duplicate and near-duplicate reads, which can consume computational resources in downstream applications. A tool that collapses such reads should reduce storage and assembly complications and costs. Results: We developed Fulcrum to collapse ident...