In Vitro Evaluation of Different Prebiotics on the Modulation of Gut Microbiota Composition and Function in Morbid Obese and Normal-Weight Subjects

The gut microbiota remains relatively stable during adulthood; however, certain intrinsic and environmental factors can lead to microbiota dysbiosis. Its restoration towards a healthy condition using best-suited prebiotics requires previous development of in vitro models for evaluating their functio...

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Published inInternational Journal of Molecular Sciences Vol. 21; no. 3; p. 906
Main Authors Nogacka, Alicja M., Salazar, Nuria, Arboleya, Silvia, Ruas-Madiedo, Patricia, Mancabelli, Leonardo, Suarez, Adolfo, Martinez-Faedo, Ceferino, Ventura, Marco, Tochio, Takumi, Hirano, Katsuaki, Endo, Akihito, G. de los Reyes-Gavilán, Clara, Gueimonde, Miguel
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 30.01.2020
MDPI
Subjects
Adult
Bacteria
Bifidobacterial-ITS
Carbohydrates
Carbon
Case-Control Studies
Feces
Female
Fermentation
Functionality
Gas production
Gases
Gastrointestinal Microbiome
Glucose
Gut microbiota
HT29
Humans
In vitro model
In vitro models
In Vitro Techniques
Male
Metabolism
Microbiota
Microorganisms
Obesity
Obesity, Morbid
Population
Prebiotic
Prebiotics
RTCA
SCFA
Thinness
Weight control
microbiota
in vitro model
HT29
gas production
RTCA
prebiotics
functionality
SCFA
bifidobacterial-ITS
obesity
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Abstract The gut microbiota remains relatively stable during adulthood; however, certain intrinsic and environmental factors can lead to microbiota dysbiosis. Its restoration towards a healthy condition using best-suited prebiotics requires previous development of in vitro models for evaluating their functionality. Herein, we carried out fecal cultures with microbiota from healthy normal-weight and morbid obese adults. Cultures were supplemented with different inulin-type fructans (1-kestose, Actilight, P95, Synergy1 and Inulin) and a galactooligosaccharide. Their impact on the gut microbiota was assessed by monitoring gas production and evaluating changes in the microbiota composition (qPCR and 16S rRNA gene profiling) and metabolic activity (gas chromatography). Additionally, the effect on the bifidobacterial species was assessed (ITS-sequencing). Moreover, the functionality of the microbiota before and after prebiotic-modulation was determined in an in vitro model of interaction with an intestinal cell line. In general, 1-kestose was the compound showing the largest effects. The modulation with prebiotics led to significant increases in the Bacteroides group and Faecalibacterium in obese subjects, whereas in normal-weight individuals, substantial rises in Bifidobacterium and Faecalibacterium were appreciated. Notably, the results obtained showed differences in the responses among the tested compounds but also among the studied human populations, indicating the need for developing population-specific products.
AbstractList The gut microbiota remains relatively stable during adulthood; however, certain intrinsic and environmental factors can lead to microbiota dysbiosis. Its restoration towards a healthy condition using best-suited prebiotics requires previous development of in vitro models for evaluating their functionality. Herein, we carried out fecal cultures with microbiota from healthy normal-weight and morbid obese adults. Cultures were supplemented with different inulin-type fructans (1-kestose, Actilight, P95, Synergy1 and Inulin) and a galactooligosaccharide. Their impact on the gut microbiota was assessed by monitoring gas production and evaluating changes in the microbiota composition (qPCR and 16S rRNA gene profiling) and metabolic activity (gas chromatography). Additionally, the effect on the bifidobacterial species was assessed (ITS-sequencing). Moreover, the functionality of the microbiota before and after prebiotic-modulation was determined in an in vitro model of interaction with an intestinal cell line. In general, 1-kestose was the compound showing the largest effects. The modulation with prebiotics led to significant increases in the Bacteroides group and Faecalibacterium in obese subjects, whereas in normal-weight individuals, substantial rises in Bifidobacterium and Faecalibacterium were appreciated. Notably, the results obtained showed differences in the responses among the tested compounds but also among the studied human populations, indicating the need for developing population-specific products.
The gut microbiota remains relatively stable during adulthood; however, certain intrinsic and environmental factors can lead to microbiota dysbiosis. Its restoration towards a healthy condition using best-suited prebiotics requires previous development of in vitro models for evaluating their functionality. Herein, we carried out fecal cultures with microbiota from healthy normal-weight and morbid obese adults. Cultures were supplemented with different inulin-type fructans (1-kestose, Actilight, P95, Synergy1 and Inulin) and a galactooligosaccharide. Their impact on the gut microbiota was assessed by monitoring gas production and evaluating changes in the microbiota composition (qPCR and 16S rRNA gene profiling) and metabolic activity (gas chromatography). Additionally, the effect on the bifidobacterial species was assessed (ITS-sequencing). Moreover, the functionality of the microbiota before and after prebiotic-modulation was determined in an in vitro model of interaction with an intestinal cell line. In general, 1-kestose was the compound showing the largest effects. The modulation with prebiotics led to significant increases in the Bacteroides group and Faecalibacterium in obese subjects, whereas in normal-weight individuals, substantial rises in Bifidobacterium and Faecalibacterium were appreciated. Notably, the results obtained showed differences in the responses among the tested compounds but also among the studied human populations, indicating the need for developing population-specific products.
The gut microbiota remains relatively stable during adulthood; however, certain intrinsic and environmental factors can lead to microbiota dysbiosis. Its restoration towards a healthy condition using best-suited prebiotics requires previous development of in vitro models for evaluating their functionality. Herein, we carried out fecal cultures with microbiota from healthy normal-weight and morbid obese adults. Cultures were supplemented with different inulin-type fructans (1-kestose, Actilight, P95, Synergy1 and Inulin) and a galactooligosaccharide. Their impact on the gut microbiota was assessed by monitoring gas production and evaluating changes in the microbiota composition (qPCR and 16S rRNA gene profiling) and metabolic activity (gas chromatography). Additionally, the effect on the bifidobacterial species was assessed (ITS-sequencing). Moreover, the functionality of the microbiota before and after prebiotic-modulation was determined in an in vitro model of interaction with an intestinal cell line. In general, 1-kestose was the compound showing the largest effects. The modulation with prebiotics led to significant increases in the group and in obese subjects, whereas in normal-weight individuals, substantial rises in and were appreciated. Notably, the results obtained showed differences in the responses among the tested compounds but also among the studied human populations, indicating the need for developing population-specific products.
The gut microbiota remains relatively stable during adulthood; however, certain intrinsic and environmental factors can lead to microbiota dysbiosis. Its restoration towards a healthy condition using best-suited prebiotics requires previous development of in vitro models for evaluating their functionality. Herein, we carried out fecal cultures with microbiota from healthy normal-weight and morbid obese adults. Cultures were supplemented with different inulin-type fructans (1-kestose, Actilight, P95, Synergy1 and Inulin) and a galactooligosaccharide. Their impact on the gut microbiota was assessed by monitoring gas production and evaluating changes in the microbiota composition (qPCR and 16S rRNA gene profiling) and metabolic activity (gas chromatography). Additionally, the effect on the bifidobacterial species was assessed (ITS-sequencing). Moreover, the functionality of the microbiota before and after prebiotic-modulation was determined in an in vitro model of interaction with an intestinal cell line. In general, 1-kestose was the compound showing the largest effects. The modulation with prebiotics led to significant increases in the Bacteroides group and Faecalibacterium in obese subjects, whereas in normal-weight individuals, substantial rises in Bifidobacterium and Faecalibacterium were appreciated. Notably, the results obtained showed differences in the responses among the tested compounds but also among the studied human populations, indicating the need for developing population-specific products.The gut microbiota remains relatively stable during adulthood; however, certain intrinsic and environmental factors can lead to microbiota dysbiosis. Its restoration towards a healthy condition using best-suited prebiotics requires previous development of in vitro models for evaluating their functionality. Herein, we carried out fecal cultures with microbiota from healthy normal-weight and morbid obese adults. Cultures were supplemented with different inulin-type fructans (1-kestose, Actilight, P95, Synergy1 and Inulin) and a galactooligosaccharide. Their impact on the gut microbiota was assessed by monitoring gas production and evaluating changes in the microbiota composition (qPCR and 16S rRNA gene profiling) and metabolic activity (gas chromatography). Additionally, the effect on the bifidobacterial species was assessed (ITS-sequencing). Moreover, the functionality of the microbiota before and after prebiotic-modulation was determined in an in vitro model of interaction with an intestinal cell line. In general, 1-kestose was the compound showing the largest effects. The modulation with prebiotics led to significant increases in the Bacteroides group and Faecalibacterium in obese subjects, whereas in normal-weight individuals, substantial rises in Bifidobacterium and Faecalibacterium were appreciated. Notably, the results obtained showed differences in the responses among the tested compounds but also among the studied human populations, indicating the need for developing population-specific products.
Author Akihito Endo
Clara G. de los Reyes-Gavilán
Marco Ventura
Silvia Arboleya
Adolfo Suárez
Patricia Ruas-Madiedo
Takumi Tochio
Leonardo Mancabelli
Alicja Nogacka
Ceferino Martinez-Faedo
Nuria Salazar
Miguel Gueimonde
Katsuaki Hirano
AuthorAffiliation 3 Functionality and Ecology of Beneficial Microorganisms, Institute of Health Research of the Principality of Asturias (ISPA), 33011 Oviedo, Spain
8 β-Food Sciences Co., Chita 478-0046, Japan; t-tochio@bfsci.co.jp (T.T.); k-hirano@bfsci.co.jp (K.H.)
5 Digestive Service, Central University Hospital of Asturias (HUCA), 33011 Oviedo, Asturias, Spain
1 Department of Microbiology and Biochemistry of Dairy Products, Instituto de Productos Lácteos de Asturias (IPLA-CSIC), 33300 Villaviciosa, Asturias, Spain; nuriasg@ipla.csic.es (N.S.); silvia.arboleya@ipla.csic.es (S.A.); ruas-madiedo@ipla.csic.es (P.R.-M.); greyes_gavilan@ipla.csic.es (C.G.d.l.R.-G.); mgueimonde@ipla.csic.es (M.G.)
4 Laboratory of Probiogenomics, Department of Life Sciences, University of Parma, 43121 Parma, Italy; leonardo.mancabelli@genprobio.com (L.M.); marco.ventura@unipr.it (M.V.)
9 Department of Food and Cosmetic Science, Tokyo University of Agriculture, Abashiri 099-2493, Japan; a3endou@nodai.ac.jp
6 Endocrinology and Nutr
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ContentType Journal Article
Contributor Ventura, Marco [0000-0002-4875-4560]
Suarez, A
Salazar, N
Nogacka, Alicja [0000-0001-8300-6149]
Ministerio de Ciencia, Innovación y Universidades (España)
Nogacka, Alicja
Arboleya, S
European Commission
Hirano, K
Endo, A
Ventura, M
Gueimonde Fernández, Miguel
Martinez-Faedo, C
Gueimonde Fernández, Miguel [0000-0002-0192-901X]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
Nogacka, A. M
Salazar, Nuria [0000-0003-1435-7628]
Ruas-Madiedo, Patricia [0000-0001-6158-9320]
Ruas-Madiedo, P
González de los Reyes-Gavilán, Clara
Mancabelli, L
de los Reyes-Gavilan, C. G
Tochio, T
Salazar, Nuria
Ruas-Madiedo, Patricia
Gueimonde, M
Ministerio de Economía y Competitividad (España)
González de los Reyes-Gavilán, Clara [0000-0001-9396-631]
Arboleya, Silvia
Mancabelli, Leonardo [0000-0002-1744-2214]
Ventura, Marco
Mancabelli, Leonardo
Arboleya, Silvia [0000-0002-6155-5822]
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Copyright 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
2020 by the authors. 2020
Copyright_xml – notice: 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
– notice: 2020 by the authors. 2020
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Issue 3
Keywords microbiota
in vitro model
HT29
gas production
RTCA
prebiotics
functionality
SCFA
bifidobacterial-ITS
obesity
Language English
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  ident: ref_23
  article-title: Insight into the prebiotic concept: Lessons from an exploratory, double blind intervention study with inulin-type fructans in obese women
  publication-title: Gut
  doi: 10.1136/gutjnl-2012-303304
– ident: ref_48
  doi: 10.3390/foods7090140
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Snippet The gut microbiota remains relatively stable during adulthood; however, certain intrinsic and environmental factors can lead to microbiota dysbiosis. Its...
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SubjectTerms Adult
Bacteria
Bifidobacterial-ITS
Carbohydrates
Carbon
Case-Control Studies
Feces
Female
Fermentation
Functionality
Gas production
Gases
Gastrointestinal Microbiome
Glucose
Gut microbiota
HT29
Humans
In vitro model
In vitro models
In Vitro Techniques
Male
Metabolism
Microbiota
Microorganisms
Obesity
Obesity, Morbid
Population
Prebiotic
Prebiotics
RTCA
SCFA
Thinness
Weight control
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Title In Vitro Evaluation of Different Prebiotics on the Modulation of Gut Microbiota Composition and Function in Morbid Obese and Normal-Weight Subjects
URI https://cir.nii.ac.jp/crid/1872835442582000512
https://www.ncbi.nlm.nih.gov/pubmed/32019174
https://www.proquest.com/docview/2548671375
https://www.proquest.com/docview/2351519579
https://pubmed.ncbi.nlm.nih.gov/PMC7038051
Volume 21
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