Gut Microbiota Contributes to the Growth of Fast-Growing Transgenic Common Carp (Cyprinus carpio L.)

• Published in: PloS one Vol. 8; no. 5; p. e64577
• Main Authors: Li, Xuemei, Yan, Qingyun, Xie, Shouqi, Hu, Wei, Yu, Yuhe, Hu, Zihua
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 31.05.2013; Public Library of Science (PLoS)
• Subjects: Animals; Animals, Genetically Modified; Bacteria; Bacteroidetes; Bacteroidetes - classification; Bacteroidetes - genetics; Biology; Body Size; Carbohydrate Metabolism; Carbohydrates; Carp; Carps - genetics; Carps - growth & development; Carps - metabolism; Carps - microbiology; Ctenopharyngodon idella; Cyprinus carpio; DNA fingerprinting; Energy consumption; Energy metabolism; Energy utilization; Fingerprinting; Firmicutes; Fish; Fusobacteria - classification; Fusobacteria - genetics; Gastrointestinal Tract - microbiology; Gene sequencing; High-Throughput Nucleotide Sequencing; Immunity; Intestinal microflora; Life Cycle Stages - physiology; Lipid Metabolism; Metabolism; Metagenome; Microbiota; Microbiota - genetics; Next-generation sequencing; Obesity; Phylogeny; Proteobacteria - classification; Proteobacteria - genetics; Relative abundance; RNA, Ribosomal, 16S - classification; RNA, Ribosomal, 16S - genetics; rRNA 16S; Studies; Transgenic fish; New York; United States > US; China
• DOI: 10.1371/journal.pone.0064577
• ISSN: 1932-6203

Gut microbiota has shown tight and coordinated connection with various functions of its host such as metabolism, immunity, energy utilization, and health maintenance. To gain insight into whether gut microbes affect the metabolism of fish, we employed fast-growing transgenic common carp (Cyprinus carpio L.) to study the connections between its large body feature and gut microbes. Metagenome-based fingerprinting and high-throughput sequencing on bacterial 16S rRNA genes indicated that fish gut was dominated by Proteobacteria, Fusobacteria, Bacteroidetes and Firmicutes, which displayed significant differences between transgenic fish and wild-type controls. Analyses to study the association of gut microbes with the fish metabolism discovered three major phyla having significant relationships with the host metabolic factors. Biochemical and histological analyses indicated transgenic fish had increased carbohydrate but decreased lipid metabolisms. Additionally, transgenic fish has a significantly lower Bacteroidetes:Firmicutes ratio than that of wild-type controls, which is similar to mammals between obese and lean individuals. These findings suggest that gut microbiotas are associated with the growth of fast growing transgenic fish, and the relative abundance of Firmicutes over Bacteroidetes could be one of the factors contributing to its fast growth. Since the large body size of transgenic fish displays a proportional body growth, which is unlike obesity in human, the results together with the findings from others also suggest that the link between obesity and gut microbiota is likely more complex than a simple Bacteroidetes:Firmicutes ratio change.