FLIM FRET Technology for Drug Discovery: Automated Multiwell-Plate High-Content Analysis, Multiplexed Readouts and Application in Situ

• Published in: Chemphyschem Vol. 12; no. 3; pp. 609 - 626
• Main Authors: Kumar, Sunil, Alibhai, Dominic, Margineanu, Anca, Laine, Romain, Kennedy, Gordon, McGinty, James, Warren, Sean, Kelly, Douglas, Alexandrov, Yuriy, Munro, Ian, Talbot, Clifford, Stuckey, Daniel W., Kimberly, Christopher, Viellerobe, Bertrand, Lacombe, Francois, Lam, Eric W.-F., Taylor, Harriet, Dallman, Margaret J., Stamp, Gordon, Murray, Edward J., Stuhmeier, Frank, Sardini, Alessandro, Katan, Matilda, Elson, Daniel S., Neil, Mark A. A., Dunsby, Chris, French, Paul M. W.
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 25.02.2011; WILEY‐VCH Verlag; Wiley
• Subjects: Biological and medical sciences; Cell Line; Chemistry; drug discovery; Drug Evaluation, Preclinical; Exact sciences and technology; fluorescence lifetime imaging; Fluorescence Resonance Energy Transfer - methods; Fluorescent Dyes - chemistry; FRET; Fundamental and applied biological sciences. Psychology; gag Gene Products, Human Immunodeficiency Virus - analysis; General and physical chemistry; Green Fluorescent Proteins - chemistry; high-throughput screening; Humans; Medical sciences; Microscopy, Fluorescence; Pharmacology. Drug treatments; Protein Binding; proteins; Proteins - analysis; Rhodamines - chemistry; High throughput screening; Förster resonance energy transfer; In situ; Fluorescence; Molecular interaction; Potential; Aggregation; Assay; drug discovery; Efficiency; Technology; Pisces; Imaging; proteins; FRET; Drug; Automation; high-throughput screening; Method; Protein; Plate; Vertebrata; Brachydanio rerio; Lifetime; Mammalia; fluorescence lifetime imaging; Microscopy; Content; End; Application

A fluorescence lifetime imaging (FLIM) technology platform intended to read out changes in Förster resonance energy transfer (FRET) efficiency is presented for the study of protein interactions across the drug‐discovery pipeline. FLIM provides a robust, inherently ratiometric imaging modality for dr...