Demineralized bone matrix gelatin as scaffold for osteochondral tissue engineering

• Published in: Biomaterials Vol. 27; no. 11; pp. 2426 - 2433
• Main Authors: Li, Xudong, Jin, Li, Balian, Gary, Laurencin, Cato T., Greg Anderson, D.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 01.04.2006
• Subjects: Animals; Base Sequence; Bone Demineralization Technique; Bone graft; Bone Matrix; Cartilage - cytology; Cartilage - metabolism; Cartilage tissue engineering; Cell culture; Cell Survival; Cells, Cultured; Chondrocyte; Chondrocytes - cytology; Chondrocytes - metabolism; Collagen; Collagen Type I - genetics; Collagen Type I - metabolism; Collagen Type II - genetics; Collagen Type II - metabolism; Gelatin; Microscopy, Electron; Rabbits; RNA, Messenger - genetics; RNA, Messenger - metabolism; Tissue Engineering - methods; Cartilage tissue engineering; Cell culture; Chondrocyte; Bone graft; Collagen
• ISSN: 0142-9612; 1878-5905
• DOI: 10.1016/j.biomaterials.2005.11.040

To develop a single-unit osteochondral tissue with demineralized bone matrix gelatin (BMG), rabbit chondrocytes were cultured on demineralized bone matrix gelatin for 6 weeks. The engineered osteochondral tissue was characterized with histology, immunolocalization, TEM, SEM, biochemical assay, and gene expression analysis. About 1.3 mm viable neo-cartilage was produced on demineralized BMG. RT-PCR, immunohistochemistry, TEM, biochemical assay, and histology revealed hyaline-like cartilage with zonal layers, intense type II collagen expression, and abundant proteoglycan content formed upon BMG compared with normal cartilage. But hydroxyproline content and type I collagen gene and protein expressions were significantly lower. We consider engineering cartilage tissue with chondrocytes cultured on allogenic demineralized BMG is a good approach for osteochondral tissue engineering.