Wheat Gluten Regulates Cholesterol Metabolism by Modulating Gut Microbiota in Hamsters with Hyperlipidemia

• Published in: Journal of Oleo Science Vol. 68; no. 9; pp. 909 - 922
• Main Authors: Liang, Ting-ting, Tong, Li-tao, Geng, Dong-hui, Wang, Li-li, Zhou, Xian-rong, Pu, Hua-yin, Jia, Wei, Wu, Qing-ping, Huang, Jun-rong
• Format: Journal Article
• Language: English
• Published: Japan Japan Oil Chemists' Society 2019; Japan Science and Technology Agency
• Subjects: Abundance; Animals; Anticholesteremic Agents - pharmacology; Bile; Casein; Cholesterol; cholesterol metabolism; Cholesterol, LDL - blood; Cholesterol, LDL - metabolism; Fatty acids; Fatty Acids, Volatile - metabolism; Feces - microbiology; Gastrointestinal Microbiome - drug effects; Gastrointestinal Microbiome - genetics; Gene sequencing; Gluten; Glutens - pharmacology; gut microbiota; Hamsters; Hyperlipidemias - microbiology; hypocholesterolemia; Lipids; Male; Mesocricetus; Metabolism; Microbiota; RNA, Ribosomal, 16S - analysis; Triglycerides; Triticum - chemistry; Wheat; wheat gluten; wheat gluten; hypocholesterolemia; cholesterol metabolism; gut microbiota
• ISSN: 1345-8957; 1347-3352
• DOI: 10.5650/jos.ess18257

The objective of this research was to evaluate the effect of wheat gluten on gut microbiota from hamsters and also analyse whether alterations in microbiota could result in wheat gluten’s lipid-lowering properties. Four weeks male hamsters were divided into 3 groups (n=10). Two hypercholesterolemic groups were fed for 35 days with hypercholesterolemic diet, containing 20% (w/w) wheat gluten or casein. Wheat gluten significantly reduced serum total cholesterol (TC), low density lipoprotein cholesterol (LDL-C) concentrations, and also decreased the liver total cholesterol (TC), free cholesterol (FC), cholesterol ester (CE), triglycerides (TG) concentrations. Wheat gluten group had a higher fecal lipids, total cholesterol (TC) and bile acids (BA) than that of casein group (p < 0.05). Moreover, wheat gluten significantly increased total short-chain fatty acids (SCFA) concentrations in feces. Sequencing of 16S rRNA gene revealed that intake of wheat gluten decreased the relative abundances of Firmicutes and Erysipelotrichaceae, but to increased the relative abundances of Bateroidetes, Bacteroidales_S24-7_group and Ruminococcaceae. The lipid lowering properties of wheat gluten was associated with the lower ratio of Firmicutes/Bateroidetes, the lower of the bacterial taxa Erysipelotrichaceae and the higher of the bacterial taxa Bacteroidales_S24-7_group and Ruminococcaceae. These results suggest that wheat gluten modulate cholesterol metabolism by altering intestinal microflora.