A maceration technique for soft plant tissue without hazardous chemicals

• Published in: Applications in plant sciences Vol. 11; no. 5; pp. e11543 - n/a
• Main Authors: Klahs, Phillip C., McMurchie, Elizabeth K., Nikkel, Jordan J., Clark, Lynn G.
• Format: Journal Article
• Language: English
• Published: United States John Wiley & Sons, Inc 01.09.2023; John Wiley and Sons Inc; Wiley
• Subjects: cell isolation; cost effectiveness; Dissection; Enzymes; epidermal peel; Epidermis; herbaria; Leaves; maceration; Microscopy; pectinase; plant tissues; polygalacturonase; Protocol Note; maceration; pectinase; cell isolation; dissection; epidermal peel

Current methods for maceration of plant tissue use hazardous chemicals. The new method described here improves the safety of dissection and maceration of soft plant tissues for microscopic imaging by using the harmless enzyme pectinase. Leaf material from a variety of land plants was obtained from l...