High Developmental Rates of Mouse Oocytes Cryopreserved by an Optimized Vitrification Protocol: The Effects of Cryoprotectants, Calcium and Cumulus Cells

• Published in: Journal of Reproduction and Development Vol. 57; no. 6; pp. 675 - 680
• Main Authors: KOHAYA, Natsuki, FUJIWARA, Katsuyoshi, ITO, Junya, KASHIWAZAKI, Naomi
• Format: Journal Article
• Language: English
• Published: Japan THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT 01.12.2011
• Subjects: Animals; Blastocyst - drug effects; Calcium - pharmacology; Cryopreservation - methods; Cryoprotective Agents - pharmacology; Cumulus Cells - drug effects; Dimethyl Sulfoxide - pharmacology; Ethylene Glycol - pharmacology; Female; In vitro fertilization; Mice; Mouse; Oocyte; Oocytes - cytology; Oocytes - drug effects; Oocytes - physiology; Vitrification; Vitrification - drug effects

Unfertilized oocytes are one of the most desired germ cell stages for cryopreservation because these cryopreserved oocytes can be used for assisted reproductive technologies, including in vitro fertilization (IVF) and intracytoplasmic sperm injection. However, in general, the fertility and developme...