Coupling endonucleases with DNA end–processing enzymes to drive gene disruption

• Published in: Nature methods Vol. 9; no. 10; pp. 973 - 975
• Main Authors: Certo, Michael T, Gwiazda, Kamila S, Kuhar, Ryan, Sather, Blythe, Curinga, Gabrielle, Mandt, Tyler, Brault, Michelle, Lambert, Abigail R, Baxter, Sarah K, Jacoby, Kyle, Ryu, Byoung Y, Kiem, Hans-Peter, Gouble, Agnes, Paques, Frederic, Rawlings, David J, Scharenberg, Andrew M
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.10.2012; Nature Publishing Group
• Subjects: 631/1647/1513; 631/1647/338/552; 631/337/1427/2191; Animals; Bioinformatics; Biological Microscopy; Biological Techniques; Biomedical and Life Sciences; Biomedical Engineering/Biotechnology; brief-communication; Cellular biology; Deoxyribonucleic acid; DNA; DNA Breaks, Double-Stranded; DNA damage; DNA End-Joining Repair; DNA Repair; Endonucleases - physiology; Enzymes; Exodeoxyribonucleases - physiology; Gene expression; HEK293 Cells; Humans; Life Sciences; Mice; Mutagenesis; Phosphoproteins - physiology; Proteomics; Receptors, CCR5 - physiology; Restriction enzymes, DNA; United States
• ISSN: 1548-7091; 1548-7105; 1548-7105
• DOI: 10.1038/nmeth.2177

Coexpression of DNA end–processing enzymes with targeted nucleases improves the efficiency of gene disruption in mammalian cells. Targeted DNA double-strand breaks introduced by rare-cleaving designer endonucleases can be harnessed for gene disruption applications by engaging mutagenic nonhomologous...