The extracellular matrix protein Agrin is expressed by osteoblasts and contributes to their differentiation

The extracellular matrix protein Agrin has been detected in chondrocytes and endosteal osteoblasts but its function in osteoblast differentiation has not been investigated yet. Thus, it is possible that Agrin contributes to osteoblast differentiation and, due to Agrin and wingless-related integratio...

Full description

Saved in:
Bibliographic Details
Published inCell and tissue research Vol. 386; no. 2; pp. 335 - 347
Main Authors Souza, Alann Thaffarell Portilho, Lopes, Helena Bacha, Oliveira, Fabiola Singaretti, Weffort, Denise, Freitas, Gileade Pereira, Adolpho, Leticia Faustino, Fernandes, Roger Rodrigo, Rosa, Adalberto Luiz, Beloti, Marcio Mateus
Format Journal Article
LanguageEnglish
Published Berlin/Heidelberg Springer Berlin Heidelberg 01.11.2021
Springer
Springer Nature B.V
Subjects
3T3 Cells
Agrin
Agrin - analysis
Agrin - genetics
Animals
Biomedical and Life Sciences
Biomedicine
Bone morphogenetic proteins
Cell Differentiation
Cells, Cultured
Chondrocytes
Chondrogenesis
Dystroglycan
Extracellular matrix
Gene Expression Regulation
Genes
Human Genetics
Humans
low density lipoprotein
Low density lipoproteins
Matrix protein
Mice
Mice, Inbred C57BL
Molecular Medicine
Osteoblastogenesis
Osteoblasts
Osteoblasts - cytology
Osteoblasts - metabolism
Osteogenesis
Proteins
Proteomics
Receptor density
Regular Article
Signal transduction
therapeutics
Wnt protein
Osteoblast
Bone
BMP
Wnt
Agrin
Online AccessGet full text
ISSN0302-766X
1432-0878
1432-0878
DOI10.1007/s00441-021-03494-9

Cover

Abstract The extracellular matrix protein Agrin has been detected in chondrocytes and endosteal osteoblasts but its function in osteoblast differentiation has not been investigated yet. Thus, it is possible that Agrin contributes to osteoblast differentiation and, due to Agrin and wingless-related integration site (Wnt) sharing the same receptor, transmembrane low-density lipoprotein receptor-related protein 4 (Lrp4), and the crosstalk between Wnt and bone morphogenetic protein (BMP) signalling, both pathways could be involved in this Agrin-mediated osteoblast differentiation. Confirming this, Agrin and its receptors Lrp4 and α-dystroglycan (Dag1) were expressed during differentiation of osteoblasts from three different sources. Moreover, the disruption of Agrin impaired the expression of its receptors and osteoblast differentiation, and the treatment with recombinant Agrin slightly increase this process. In addition, whilst Agrin knockdown downregulated the expression of genes related to Wnt and BMP signalling pathways, the addition of Agrin had no effect on these genes. Altogether, these data uncover the contribution of Agrin to osteoblast differentiation and suggest that, at least in part, an Agrin-Wnt-BMP circuit is involved in this process. This makes Agrin a candidate as target for developing new therapeutic strategies to treat bone-related diseases and injuries.
AbstractList The extracellular matrix protein Agrin has been detected in chondrocytes and endosteal osteoblasts but its function in osteoblast differentiation has not been investigated yet. Thus, it is possible that Agrin contributes to osteoblast differentiation and, due to Agrin and wingless-related integration site (Wnt) sharing the same receptor, transmembrane low-density lipoprotein receptor-related protein 4 (Lrp4), and the crosstalk between Wnt and bone morphogenetic protein (BMP) signalling, both pathways could be involved in this Agrin-mediated osteoblast differentiation. Confirming this, Agrin and its receptors Lrp4 and α-dystroglycan (Dag1) were expressed during differentiation of osteoblasts from three different sources. Moreover, the disruption of Agrin impaired the expression of its receptors and osteoblast differentiation, and the treatment with recombinant Agrin slightly increase this process. In addition, whilst Agrin knockdown downregulated the expression of genes related to Wnt and BMP signalling pathways, the addition of Agrin had no effect on these genes. Altogether, these data uncover the contribution of Agrin to osteoblast differentiation and suggest that, at least in part, an Agrin-Wnt-BMP circuit is involved in this process. This makes Agrin a candidate as target for developing new therapeutic strategies to treat bone-related diseases and injuries.
The extracellular matrix protein Agrin has been detected in chondrocytes and endosteal osteoblasts but its function in osteoblast differentiation has not been investigated yet. Thus, it is possible that Agrin contributes to osteoblast differentiation and, due to Agrin and wingless-related integration site (Wnt) sharing the same receptor, transmembrane low-density lipoprotein receptor-related protein 4 (Lrp4), and the crosstalk between Wnt and bone morphogenetic protein (BMP) signalling, both pathways could be involved in this Agrin-mediated osteoblast differentiation. Confirming this, Agrin and its receptors Lrp4 and [alpha]-dystroglycan (Dag1) were expressed during differentiation of osteoblasts from three different sources. Moreover, the disruption of Agrin impaired the expression of its receptors and osteoblast differentiation, and the treatment with recombinant Agrin slightly increase this process. In addition, whilst Agrin knockdown downregulated the expression of genes related to Wnt and BMP signalling pathways, the addition of Agrin had no effect on these genes. Altogether, these data uncover the contribution of Agrin to osteoblast differentiation and suggest that, at least in part, an Agrin-Wnt-BMP circuit is involved in this process. This makes Agrin a candidate as target for developing new therapeutic strategies to treat bone-related diseases and injuries.
The extracellular matrix protein Agrin has been detected in chondrocytes and endosteal osteoblasts but its function in osteoblast differentiation has not been investigated yet. Thus, it is possible that Agrin contributes to osteoblast differentiation and, due to Agrin and wingless-related integration site (Wnt) sharing the same receptor, transmembrane low-density lipoprotein receptor-related protein 4 (Lrp4), and the crosstalk between Wnt and bone morphogenetic protein (BMP) signalling, both pathways could be involved in this Agrin-mediated osteoblast differentiation. Confirming this, Agrin and its receptors Lrp4 and α-dystroglycan (Dag1) were expressed during differentiation of osteoblasts from three different sources. Moreover, the disruption of Agrin impaired the expression of its receptors and osteoblast differentiation, and the treatment with recombinant Agrin slightly increase this process. In addition, whilst Agrin knockdown downregulated the expression of genes related to Wnt and BMP signalling pathways, the addition of Agrin had no effect on these genes. Altogether, these data uncover the contribution of Agrin to osteoblast differentiation and suggest that, at least in part, an Agrin-Wnt-BMP circuit is involved in this process. This makes Agrin a candidate as target for developing new therapeutic strategies to treat bone-related diseases and injuries.The extracellular matrix protein Agrin has been detected in chondrocytes and endosteal osteoblasts but its function in osteoblast differentiation has not been investigated yet. Thus, it is possible that Agrin contributes to osteoblast differentiation and, due to Agrin and wingless-related integration site (Wnt) sharing the same receptor, transmembrane low-density lipoprotein receptor-related protein 4 (Lrp4), and the crosstalk between Wnt and bone morphogenetic protein (BMP) signalling, both pathways could be involved in this Agrin-mediated osteoblast differentiation. Confirming this, Agrin and its receptors Lrp4 and α-dystroglycan (Dag1) were expressed during differentiation of osteoblasts from three different sources. Moreover, the disruption of Agrin impaired the expression of its receptors and osteoblast differentiation, and the treatment with recombinant Agrin slightly increase this process. In addition, whilst Agrin knockdown downregulated the expression of genes related to Wnt and BMP signalling pathways, the addition of Agrin had no effect on these genes. Altogether, these data uncover the contribution of Agrin to osteoblast differentiation and suggest that, at least in part, an Agrin-Wnt-BMP circuit is involved in this process. This makes Agrin a candidate as target for developing new therapeutic strategies to treat bone-related diseases and injuries.
Audience Academic
Author Weffort, Denise
Fernandes, Roger Rodrigo
Rosa, Adalberto Luiz
Beloti, Marcio Mateus
Freitas, Gileade Pereira
Souza, Alann Thaffarell Portilho
Lopes, Helena Bacha
Oliveira, Fabiola Singaretti
Adolpho, Leticia Faustino
Author_xml – sequence: 1
  givenname: Alann Thaffarell Portilho
  orcidid: 0000-0001-7344-5420
  surname: Souza
  fullname: Souza, Alann Thaffarell Portilho
  organization: Bone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo
– sequence: 2
  givenname: Helena Bacha
  orcidid: 0000-0002-6998-5296
  surname: Lopes
  fullname: Lopes, Helena Bacha
  organization: Bone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo
– sequence: 3
  givenname: Fabiola Singaretti
  orcidid: 0000-0003-0855-5118
  surname: Oliveira
  fullname: Oliveira, Fabiola Singaretti
  organization: Bone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo
– sequence: 4
  givenname: Denise
  orcidid: 0000-0002-4646-8077
  surname: Weffort
  fullname: Weffort, Denise
  organization: Bone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo
– sequence: 5
  givenname: Gileade Pereira
  orcidid: 0000-0002-1674-4667
  surname: Freitas
  fullname: Freitas, Gileade Pereira
  organization: Bone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo
– sequence: 6
  givenname: Leticia Faustino
  orcidid: 0000-0002-1952-5136
  surname: Adolpho
  fullname: Adolpho, Leticia Faustino
  organization: Bone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo
– sequence: 7
  givenname: Roger Rodrigo
  orcidid: 0000-0002-9245-1823
  surname: Fernandes
  fullname: Fernandes, Roger Rodrigo
  organization: Bone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo
– sequence: 8
  givenname: Adalberto Luiz
  orcidid: 0000-0002-6495-2778
  surname: Rosa
  fullname: Rosa, Adalberto Luiz
  organization: Bone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo
– sequence: 9
  givenname: Marcio Mateus
  orcidid: 0000-0003-0149-7189
  surname: Beloti
  fullname: Beloti, Marcio Mateus
  email: mmbeloti@usp.br
  organization: Bone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo
BackLink https://www.ncbi.nlm.nih.gov/pubmed/34223979$$D View this record in MEDLINE/PubMed
BookMark eNqFkltr3DAQhUVJaTZp_0AfiqBQ-uJUlmRZflxCL4FAX1Lom5Dt8a5SW9pKMiT_PuNsQi6UFGEJzHcOM2fmiBz44IGQ9yU7KRmrvyTGpCwLxvETspFF84qsSil4wXStD8iKCcaLWqnfh-QopUvGSqlU84YcCsm5aOpmRf5cbIHCVY62g3GcRxvpZHN0V3QXQwbn6XoT8XYJqV2ElKCn7TUNKUNoR5tyotb3tAseVe2cIdEcaN6Ci7R3wwARfHY2u-DfkteDHRO8u3uPya9vXy9OfxTnP7-fna7Pi65iKhcKe8M6exBaa2nLTkNZQSVtLSrFJEJaA5KyqYVtFfBedbzUumk4s13NxTH5vPfFFv7OkLKZXFrasx7CnAxXQtVclg37P1pJrVjNm8X14zP0MszRYyNIaQxc1rJ6oDZ2BOP8EJZoF1OzVpppWeHIkDr5B4Wnh8lhlDA4_P9E8OmRYAt2zNsUxnmJNT0FP9xVObcT9GYX3WTjtbmfOAJ6D3QxpBRhMJ3Lt-PBEtxoSmaW5TL75TK4XOZ2ucwi5c-k9-4visRelBD2G4gPsb2gugHCV92d
CitedBy_id crossref_primary_10_1038_s41598_024_69629_4
crossref_primary_10_3390_ijms26031007
crossref_primary_10_1111_iep_12525
crossref_primary_10_3390_jfb14020079
crossref_primary_10_1038_s41598_024_84116_6
crossref_primary_10_1038_s41536_022_00267_y
crossref_primary_10_3390_cells12101364
crossref_primary_10_3892_ijmm_2024_5422
Cites_doi 10.1136/annrheumdis-2015-207316
10.1016/s0092-8674(01)00622-5
10.1038/nrg3185
10.1006/meth.2001.1262
10.3389/fcell.2018.00170
10.2174/138161209787846739
10.1038/nature22978
10.1016/j.coph.2018.03.005
10.1182/blood-2011-01-331272
10.1016/j.neuint.2012.02.028
10.1002/term.2738
10.1016/j.neulet.2019.134676
10.1016/j.bone.2015.05.012
10.3389/fmolb.2019.00018
10.1038/cgt.2016.4
10.1038/nrm1074
10.1146/annurev-physiol-022516-034255
10.1016/0896-6273(94)90324-7
10.1002/dvdy.22009
10.1007/s00774-015-0734-7
10.1101/cshperspect.a009167
10.1002/jcp.1041430304
10.1101/sqb.1990.055.01.041
10.3389/fimmu.2020.00212
10.1083/jcb.105.6.2471
10.1016/j.diff.2016.02.005
10.1242/jcs.02814
10.1126/scitranslmed.aax9086
10.3389/fmolb.2019.00156
10.1038/cdd.2016.10
10.1242/dev.150680
10.1126/science.1056594
10.1038/ncomms7184
10.1159/000223687
10.1007/s00018-016-2425-5
10.1089/ten.TEB.2016.0454
10.1007/s00418-006-0258-2
10.1128/MCB.00253-16
10.1371/journal.pcbi.0030057
10.1096/fj.04-3303com
10.1038/boneres.2015.5
10.1016/j.cell.2008.10.002
10.1002/jcb.20958
10.1016/j.cell.2016.03.014
10.1016/j.injury.2005.07.031
10.1016/j.bone.2012.09.029
10.1007/978-1-61779-415-5
10.21769/BioProtoc.3534
10.1371/journal.pone.0115004
ContentType Journal Article
Copyright The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021
2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
COPYRIGHT 2021 Springer
The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021.
Copyright_xml – notice: The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021
– notice: 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
– notice: COPYRIGHT 2021 Springer
– notice: The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021.
DBID AAYXX
CITATION
CGR
CUY
CVF
ECM
EIF
NPM
3V.
7QP
7QR
7RV
7SS
7TK
7X7
7XB
88A
88E
8AO
8FD
8FE
8FH
8FI
8FJ
8FK
ABUWG
AFKRA
AZQEC
BBNVY
BENPR
BHPHI
CCPQU
DWQXO
FR3
FYUFA
GHDGH
GNUQQ
HCIFZ
K9.
KB0
LK8
M0S
M1P
M7P
NAPCQ
P64
PHGZM
PHGZT
PJZUB
PKEHL
PPXIY
PQEST
PQGLB
PQQKQ
PQUKI
PRINS
RC3
7X8
7S9
L.6
DOI 10.1007/s00441-021-03494-9
DatabaseName CrossRef
Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
ProQuest Central (Corporate)
Calcium & Calcified Tissue Abstracts
Chemoreception Abstracts
Nursing & Allied Health Database
Entomology Abstracts (Full archive)
Neurosciences Abstracts
Health & Medical Collection
ProQuest Central (purchase pre-March 2016)
Biology Database (Alumni Edition)
Medical Database (Alumni Edition)
ProQuest Pharma Collection
Technology Research Database
ProQuest SciTech Collection
ProQuest Natural Science Collection
ProQuest Hospital Collection
Hospital Premium Collection (Alumni Edition)
ProQuest Central (Alumni) (purchase pre-March 2016)
ProQuest Central (Alumni)
ProQuest Central UK/Ireland
ProQuest Central Essentials
Biological Science Collection
AUTh Library subscriptions: ProQuest Central
Natural Science Collection
ProQuest One Community College
ProQuest Central Korea
Engineering Research Database
Health Research Premium Collection
Health Research Premium Collection (Alumni)
ProQuest Central Student
SciTech Premium Collection
ProQuest Health & Medical Complete (Alumni)
Nursing & Allied Health Database (Alumni Edition)
Biological Sciences
ProQuest Health & Medical Collection
Medical Database
Biological Science Database
Nursing & Allied Health Premium
Biotechnology and BioEngineering Abstracts
ProQuest Central Premium
ProQuest One Academic (New)
ProQuest Health & Medical Research Collection
ProQuest One Academic Middle East (New)
ProQuest One Health & Nursing
ProQuest One Academic Eastern Edition (DO NOT USE)
ProQuest One Applied & Life Sciences
ProQuest One Academic
ProQuest One Academic UKI Edition
ProQuest Central China
Genetics Abstracts
MEDLINE - Academic
AGRICOLA
AGRICOLA - Academic
DatabaseTitle CrossRef
MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
ProQuest Central Student
Technology Research Database
ProQuest One Academic Middle East (New)
ProQuest Central Essentials
ProQuest Health & Medical Complete (Alumni)
ProQuest Central (Alumni Edition)
SciTech Premium Collection
ProQuest One Community College
ProQuest One Health & Nursing
ProQuest Natural Science Collection
ProQuest Pharma Collection
ProQuest Central China
ProQuest Biology Journals (Alumni Edition)
ProQuest Central
ProQuest One Applied & Life Sciences
ProQuest Health & Medical Research Collection
Genetics Abstracts
Health Research Premium Collection
Health and Medicine Complete (Alumni Edition)
Natural Science Collection
ProQuest Central Korea
Health & Medical Research Collection
Biological Science Collection
Chemoreception Abstracts
ProQuest Central (New)
ProQuest Medical Library (Alumni)
ProQuest Biological Science Collection
ProQuest One Academic Eastern Edition
ProQuest Nursing & Allied Health Source
ProQuest Hospital Collection
Health Research Premium Collection (Alumni)
Biological Science Database
ProQuest SciTech Collection
Neurosciences Abstracts
ProQuest Hospital Collection (Alumni)
Biotechnology and BioEngineering Abstracts
Entomology Abstracts
Nursing & Allied Health Premium
ProQuest Health & Medical Complete
ProQuest Medical Library
ProQuest One Academic UKI Edition
ProQuest Nursing & Allied Health Source (Alumni)
Engineering Research Database
ProQuest One Academic
Calcium & Calcified Tissue Abstracts
ProQuest One Academic (New)
ProQuest Central (Alumni)
MEDLINE - Academic
AGRICOLA
AGRICOLA - Academic
DatabaseTitleList MEDLINE


ProQuest Central Student
AGRICOLA

MEDLINE - Academic
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
– sequence: 3
  dbid: BENPR
  name: ProQuest Central
  url: https://www.proquest.com/central
  sourceTypes: Aggregation Database
DeliveryMethod fulltext_linktorsrc
Discipline Biology
EISSN 1432-0878
EndPage 347
ExternalDocumentID A680845441
34223979
10_1007_s00441_021_03494_9
Genre Journal Article
GrantInformation_xml – fundername: Fundação de Amparo à Pesquisa do Estado de São Paulo
  grantid: 2016/14171-0; 2017/20349-9
  funderid: http://dx.doi.org/10.13039/501100001807
– fundername: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
  funderid: http://dx.doi.org/10.13039/501100002322
– fundername: Conselho Nacional de Desenvolvimento Científico e Tecnológico
  grantid: 303464/2016-0
  funderid: http://dx.doi.org/10.13039/501100003593
– fundername: Fundação de Amparo à Pesquisa do Estado de São Paulo
  grantid: 2017/20349-9
– fundername: Conselho Nacional de Desenvolvimento Científico e Tecnológico
  grantid: 303464/2016-0
– fundername: Fundação de Amparo à Pesquisa do Estado de São Paulo
  grantid: 2016/14171-0
GroupedDBID ---
-4W
-56
-5G
-BR
-EM
-Y2
-~C
-~X
.55
.86
.GJ
.VR
06C
06D
0R~
0VY
199
1N0
2.D
203
28-
29B
29~
2J2
2JN
2JY
2KG
2KM
2LR
2P1
2VQ
2~H
30V
36B
3O-
3SX
3V.
4.4
406
408
409
40D
40E
53G
5QI
5RE
5VS
67N
67Z
6NX
78A
7RV
7X7
88A
88E
8AO
8FE
8FH
8FI
8FJ
8TC
8UJ
95-
95.
95~
96X
AAAVM
AABHQ
AACDK
AAHBH
AAHNG
AAIAL
AAJBT
AAJKR
AANXM
AANZL
AARHV
AARTL
AASML
AATNV
AATVU
AAUYE
AAWCG
AAYIU
AAYQN
AAYTO
AAYZH
ABAKF
ABBBX
ABBXA
ABDZT
ABECU
ABFTV
ABHLI
ABHQN
ABJNI
ABJOX
ABKCH
ABKTR
ABLJU
ABMNI
ABMQK
ABNWP
ABOCM
ABPLI
ABQBU
ABQSL
ABSXP
ABTEG
ABTHY
ABTKH
ABTMW
ABULA
ABUWG
ABWNU
ABXPI
ACAOD
ACBXY
ACDTI
ACGFS
ACHSB
ACHVE
ACHXU
ACIWK
ACKNC
ACMDZ
ACMLO
ACOKC
ACOMO
ACPIV
ACPRK
ACZOJ
ADBBV
ADHIR
ADIMF
ADINQ
ADKNI
ADKPE
ADRFC
ADTPH
ADURQ
ADYFF
ADYPR
ADZKW
AEBTG
AEFIE
AEFQL
AEGAL
AEGNC
AEJHL
AEJRE
AEKMD
AEMSY
AENEX
AEOHA
AEPYU
AESKC
AETLH
AEVLU
AEXYK
AFBBN
AFDYV
AFEXP
AFGCZ
AFKRA
AFLOW
AFQWF
AFWTZ
AFZKB
AGAYW
AGDGC
AGGDS
AGJBK
AGMZJ
AGQEE
AGQMX
AGRTI
AGWIL
AGWZB
AGYKE
AHAVH
AHBYD
AHKAY
AHMBA
AHSBF
AHYZX
AIAKS
AIGIU
AIIXL
AILAN
AITGF
AJBLW
AJRNO
AJZVZ
AKMHD
ALIPV
ALMA_UNASSIGNED_HOLDINGS
ALWAN
AMKLP
AMXSW
AMYLF
AOCGG
ARMRJ
ASPBG
AVWKF
AXYYD
AZFZN
B-.
BA0
BBNVY
BBWZM
BDATZ
BENPR
BGNMA
BHPHI
BKEYQ
BPHCQ
BSONS
BVXVI
CAG
CCPQU
COF
CS3
CSCUP
DDRTE
DL5
DNIVK
DPUIP
EBD
EBLON
EBS
EIOEI
EJD
EMB
EMOBN
EN4
EPAXT
ESBYG
EX3
F5P
FEDTE
FERAY
FFXSO
FIGPU
FINBP
FNLPD
FRRFC
FSGXE
FWDCC
FYUFA
G-Y
G-Z
GGCAI
GGRSB
GJIRD
GNWQR
GQ6
GQ7
GQ8
GXS
H13
HCIFZ
HF~
HG5
HG6
HMCUK
HMJXF
HQYDN
HRMNR
HVGLF
HZ~
I09
IAO
IFM
IHE
IHR
IHW
IJ-
IKXTQ
INH
INR
ITC
ITM
IWAJR
IXC
IZIGR
IZQ
I~X
I~Z
J-C
J0Z
JBSCW
JCJTX
JZLTJ
KDC
KOV
KOW
KPH
L7B
LAS
LK8
LLZTM
M0L
M1P
M4Y
M7P
MA-
MVM
N2Q
N9A
NAPCQ
NB0
NDZJH
NPVJJ
NQJWS
NU0
O9-
O93
O9G
O9I
O9J
OAM
P19
P2P
PF0
PKN
PQQKQ
PROAC
PSQYO
PT4
PT5
Q2X
QOK
QOR
QOS
R4E
R89
R9I
RHV
RIG
RNI
RNS
ROL
RPX
RRX
RSV
RZK
S16
S1Z
S26
S27
S28
S3A
S3B
SAP
SBL
SBY
SCLPG
SDH
SDM
SHX
SISQX
SJYHP
SNE
SNPRN
SNX
SOHCF
SOJ
SPISZ
SRMVM
SSLCW
SSXJD
STPWE
SV3
SZN
T13
T16
TSG
TSK
TSV
TUC
U2A
U9L
UG4
UKHRP
UOJIU
UTJUX
UZXMN
VC2
VFIZW
W23
W48
WJK
WK6
WK8
WOW
X7M
YLTOR
Z45
Z7U
Z7W
Z82
Z83
Z87
Z8O
Z8Q
Z8V
Z8W
Z91
ZGI
ZMTXR
ZOVNA
ZXP
~EX
~KM
AAPKM
AAYXX
ABBRH
ABDBE
ABFSG
ACSTC
ADHKG
AEZWR
AFDZB
AFHIU
AFOHR
AGQPQ
AHPBZ
AHWEU
AIXLP
ATHPR
AYFIA
CITATION
PHGZM
PHGZT
CGR
CUY
CVF
ECM
EIF
NPM
AEIIB
PMFND
7QP
7QR
7SS
7TK
7XB
8FD
8FK
ABRTQ
AZQEC
DWQXO
FR3
GNUQQ
K9.
P64
PJZUB
PKEHL
PPXIY
PQEST
PQGLB
PQUKI
PRINS
RC3
7X8
PUEGO
7S9
L.6
ID FETCH-LOGICAL-c506t-6007466de38884a1c8e15e54a735604c5088e5064973ab6e2d6c21889920ac723
IEDL.DBID AGYKE
ISSN 0302-766X
1432-0878
IngestDate Thu Sep 04 17:15:35 EDT 2025
Fri Sep 05 02:15:15 EDT 2025
Fri Jul 25 19:10:22 EDT 2025
Tue Jun 17 21:25:10 EDT 2025
Tue Jun 10 20:30:29 EDT 2025
Thu May 22 21:21:39 EDT 2025
Wed Feb 19 02:27:28 EST 2025
Tue Jul 01 00:49:40 EDT 2025
Thu Apr 24 23:09:24 EDT 2025
Fri Feb 21 02:47:48 EST 2025
IsPeerReviewed true
IsScholarly true
Issue 2
Keywords Osteoblast
Bone
BMP
Wnt
Agrin
Language English
License 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c506t-6007466de38884a1c8e15e54a735604c5088e5064973ab6e2d6c21889920ac723
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
ORCID 0000-0002-6998-5296
0000-0002-6495-2778
0000-0001-7344-5420
0000-0003-0149-7189
0000-0002-4646-8077
0000-0002-9245-1823
0000-0003-0855-5118
0000-0002-1674-4667
0000-0002-1952-5136
PMID 34223979
PQID 2588784745
PQPubID 49301
PageCount 13
ParticipantIDs proquest_miscellaneous_2636724190
proquest_miscellaneous_2548607292
proquest_journals_2588784745
gale_infotracmisc_A680845441
gale_infotracacademiconefile_A680845441
gale_healthsolutions_A680845441
pubmed_primary_34223979
crossref_citationtrail_10_1007_s00441_021_03494_9
crossref_primary_10_1007_s00441_021_03494_9
springer_journals_10_1007_s00441_021_03494_9
ProviderPackageCode CITATION
AAYXX
PublicationCentury 2000
PublicationDate 20211100
2021-11-00
2021-Nov
20211101
PublicationDateYYYYMMDD 2021-11-01
PublicationDate_xml – month: 11
  year: 2021
  text: 20211100
PublicationDecade 2020
PublicationPlace Berlin/Heidelberg
PublicationPlace_xml – name: Berlin/Heidelberg
– name: Germany
– name: Heidelberg
PublicationTitle Cell and tissue research
PublicationTitleAbbrev Cell Tissue Res
PublicationTitleAlternate Cell Tissue Res
PublicationYear 2021
Publisher Springer Berlin Heidelberg
Springer
Springer Nature B.V
Publisher_xml – name: Springer Berlin Heidelberg
– name: Springer
– name: Springer Nature B.V
References Dempsey, Bigotti, Adams, Brancaccio (CR14) 2019; 6
Anselmo, Lauranzano, Soldani, Ploia, Angioni, D'amico G, Sarukhan A, Mazzon C, Viola A (CR3) 2016; 23
Gentili, Cancedda (CR21) 2009; 15
Shen, Xiong, Mei (CR47) 2015; 80
Houschyar, Tapking, Borrelli, Popp, Duscher, Maan, Chelliah, Li, Harati, Wallner, Rein, Pförringer, Reumuth, Grieb, Mouraret, Dadras, Wagner, Cha, Siemers, Lehnhardt, Behr (CR28) 2019; 6
Zhang, Oyajobi, Harris, Chen, Tsao, Deng, Zhao (CR50) 2013; 52
Livak, Schmittgen (CR40) 2001; 25
Li, Xiong, Mei (CR38) 2018; 80
CR32
Nitkin, Smith, Magill, Fallon, Yao, Wallace, McMahan (CR44) 1987; 105
Komori (CR35) 2006; 99
Bowe, Deyst, Leszyk, Fallon (CR8) 1994; 12
Zhang, Wang, Chu, Su, Gong, Zhang, Xiong (CR49) 2006; 20
Itasaki, Hoppler (CR29) 2010; 239
Blair, Larrouture, Li, Lin, Beer-Stoltz, Liu, Tuan, Robinson, Schlesinger, Nelson (CR7) 2017; 23
Guarino, Canciani, Forneris (CR24) 2020; 6
Nakashima, Zhou, Kunkel, Zhang, Deng, Behringer, de Crombrugghe (CR43) 2002; 108
Herbst (CR27) 2020; 716
Li, Zhang, Yang, Li, Dai (CR37) 2016; 34
Almalki, Agrawal (CR2) 2016; 92
Grabowski (CR22) 2009; 16
Burden, Yumoto, Zhang (CR11) 2013; 5
McMahan (CR42) 1990; 55
Bassat, Mutlak, Genzelinakh, Shadrin, Baruch Umansky, Yifa, Kain, Rajchman, Leach, Riabov Bassat, Udi, Sarig, Sagi, Martin, Bursac, Cohen, Tzahor (CR5) 2017; 547
Brockmann, Beyer, Heinisch, Wilhelm (CR9) 2007; 3
Owen, Aronow, Shalhoub, Barone, Wilming, Tassinari, Kennedy, Pockwinse, Lian, Stein (CR45) 1990; 143
Ahn, Sims, Murray, Kuhlmann, Fuentes-Antrás, Weatherbee, Krumlauf (CR1) 2017; 144
Barresi, Campbell (CR4) 2006; 119
Karner, Long (CR31) 2017; 74
Buckwalter, Cooper (CR10) 1987; 36
Mazzon, Anselmo, Cibella, Soldani, Destro, Kim, Roncalli, Burden, Dustin, Sarukhan, Viola (CR41) 2011; 118
Ebrahimi, Ostrovidov, Salehi, Kim, Bae, Khademhosseini (CR16) 2018; 12
Vogel, Marcotte (CR48) 2012; 13
Eldridge, Nalesso, Ismail, Vicente-Greco, Kabouridis, Ramachandran, Niemeier, Herz, Pitzalis, Perretti, Dell'Accio (CR17) 2016; 75
Eldridge, Barawi, Wang, Roelofs, Kaneva, Guan, Lydon, Thomas, Thorup, Fernandez, Caxaria, Strachan, Ali, Shanmuganathan, Pitzalis, Whiteford, Henson, McCaskie, De Bari, Dell’Accio (CR18) 2020; 12
Bezakova, Ruegg (CR6) 2003; 4
Kim, Stiegler, Cameron, Hallock, Gomez, Huang, Hubbard, Dustin, Burden (CR34) 2008; 135
Dimitriou, Giannoudis (CR15) 2005; 36
Khan, Bose, Yam, Soloski, Rupp (CR33) 2001; 292
Rahman, Akhtar, Jamil, Banik, Asaduzzaman (CR46) 2015; 3
Karner, Lee, Long (CR30) 2017; 37
Hausser, Ruegg, Brenner, Ksiazek (CR25) 2007; 127
Lazaridis, Tzartos (CR36) 2020; 11
CR26
Daniels (CR13) 2012; 61
Chakraborty, Lakshmanan, Swa, Chen, Zhang, Ong, Loo, Akıncılar, Gunaratne, Tergaonkar, Hui, Hong (CR12) 2015; 6
Fakhr, Zare, Teimoori-Toolabi (CR19) 2016; 23
Liu, Beyer, Aebersold (CR39) 2016; 165
CR20
Grol, Lee (CR23) 2018; 40
R Dimitriou (3494_CR15) 2005; 36
3494_CR32
MP Daniels (3494_CR13) 2012; 61
MS Rahman (3494_CR46) 2015; 3
CM Karner (3494_CR30) 2017; 37
A Anselmo (3494_CR3) 2016; 23
E Bassat (3494_CR5) 2017; 547
TA Owen (3494_CR45) 1990; 143
SR Guarino (3494_CR24) 2020; 6
RM Nitkin (3494_CR44) 1987; 105
G Bezakova (3494_CR6) 2003; 4
HJ Hausser (3494_CR25) 2007; 127
3494_CR20
UJ McMahan (3494_CR42) 1990; 55
C Gentili (3494_CR21) 2009; 15
3494_CR26
CM Karner (3494_CR31) 2017; 74
C Shen (3494_CR47) 2015; 80
S Chakraborty (3494_CR12) 2015; 6
HC Blair (3494_CR7) 2017; 23
Y Ahn (3494_CR1) 2017; 144
E Fakhr (3494_CR19) 2016; 23
N Kim (3494_CR34) 2008; 135
T Komori (3494_CR35) 2006; 99
R Herbst (3494_CR27) 2020; 716
SG Almalki (3494_CR2) 2016; 92
S Eldridge (3494_CR17) 2016; 75
SE Eldridge (3494_CR18) 2020; 12
L Li (3494_CR38) 2018; 80
K Lazaridis (3494_CR36) 2020; 11
R Brockmann (3494_CR9) 2007; 3
JA Buckwalter (3494_CR10) 1987; 36
R Barresi (3494_CR4) 2006; 119
Y Liu (3494_CR39) 2016; 165
KS Houschyar (3494_CR28) 2019; 6
MW Grol (3494_CR23) 2018; 40
R Zhang (3494_CR50) 2013; 52
MA Bowe (3494_CR8) 1994; 12
N Itasaki (3494_CR29) 2010; 239
C Vogel (3494_CR48) 2012; 13
M Ebrahimi (3494_CR16) 2018; 12
C Mazzon (3494_CR41) 2011; 118
AA Khan (3494_CR33) 2001; 292
SJ Burden (3494_CR11) 2013; 5
J Zhang (3494_CR49) 2006; 20
CE Dempsey (3494_CR14) 2019; 6
J Li (3494_CR37) 2016; 34
P Grabowski (3494_CR22) 2009; 16
KJ Livak (3494_CR40) 2001; 25
K Nakashima (3494_CR43) 2002; 108
References_xml – volume: 75
  start-page: 1228
  year: 2016
  end-page: 1235
  ident: CR17
  article-title: Agrin mediates chondrocyte homeostasis and requires both LRP4 and α-dystroglycan to enhance cartilage formation in vitro and in vivo
  publication-title: Ann Rheum Dis
  doi: 10.1136/annrheumdis-2015-207316
– volume: 108
  start-page: 17
  year: 2002
  end-page: 29
  ident: CR43
  article-title: The novel zinc finger-containing transcription factor osterix is required for osteoblast differentiation and bone formation
  publication-title: Cell
  doi: 10.1016/s0092-8674(01)00622-5
– volume: 13
  start-page: 227
  year: 2012
  end-page: 232
  ident: CR48
  article-title: Insights into the regulation of protein abundance from proteomic and transcriptomic analyses
  publication-title: Nat Rev Genet
  doi: 10.1038/nrg3185
– volume: 25
  start-page: 402
  year: 2001
  end-page: 408
  ident: CR40
  article-title: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method
  publication-title: Methods
  doi: 10.1006/meth.2001.1262
– volume: 6
  start-page: 170
  year: 2019
  ident: CR28
  article-title: Wnt pathway in bone repair and regeneration—what do we know so far
  publication-title: Front Cell Dev Biol
  doi: 10.3389/fcell.2018.00170
– volume: 15
  start-page: 1334
  year: 2009
  end-page: 1348
  ident: CR21
  article-title: Cartilage and bone extracellular matrix
  publication-title: Curr Pharm Des
  doi: 10.2174/138161209787846739
– volume: 547
  start-page: 179
  year: 2017
  end-page: 184
  ident: CR5
  article-title: The extracellular matrix protein agrin promotes heart regeneration in mice
  publication-title: Nature
  doi: 10.1038/nature22978
– volume: 40
  start-page: 59
  year: 2018
  end-page: 66
  ident: CR23
  article-title: Gene therapy for repair and regeneration of bone and cartilage
  publication-title: Curr Opin Pharmacol
  doi: 10.1016/j.coph.2018.03.005
– volume: 118
  start-page: 2733
  year: 2011
  end-page: 2742
  ident: CR41
  article-title: The critical role of agrin in the hematopoietic stem cell niche
  publication-title: Blood
  doi: 10.1182/blood-2011-01-331272
– volume: 61
  start-page: 848
  year: 2012
  end-page: 853
  ident: CR13
  article-title: The role of agrin in synaptic development, plasticity and signalling in the central nervous system
  publication-title: Neurochem Int
  doi: 10.1016/j.neuint.2012.02.028
– volume: 12
  start-page: 2151
  year: 2018
  end-page: 2163
  ident: CR16
  article-title: Enhanced skeletal muscle formation on microfluidic spun gelatin methacryloyl (GelMA) fibres using surface patterning and agrin treatment
  publication-title: J Tissue Eng Regen Med
  doi: 10.1002/term.2738
– volume: 716
  year: 2020
  ident: CR27
  article-title: MuSk function during health and disease
  publication-title: Neurosci Lett
  doi: 10.1016/j.neulet.2019.134676
– volume: 80
  start-page: 101
  year: 2015
  end-page: 108
  ident: CR47
  article-title: LRP4 in neuromuscular junction and bone development and diseases
  publication-title: Bone
  doi: 10.1016/j.bone.2015.05.012
– volume: 6
  start-page: 18
  year: 2019
  ident: CR14
  article-title: Analysis of α-dystroglycan/LG domain binding modes: investigating protein motifs that regulate the affinity of isolated LG domains
  publication-title: Front Mol Biosci
  doi: 10.3389/fmolb.2019.00018
– volume: 23
  start-page: 73
  year: 2016
  end-page: 82
  ident: CR19
  article-title: Precise and efficient siRNA design: a key point in competent gene silencing
  publication-title: Cancer Gene Ther
  doi: 10.1038/cgt.2016.4
– volume: 4
  start-page: 295
  year: 2003
  end-page: 308
  ident: CR6
  article-title: New insights into the roles of agrin
  publication-title: Nat Rev Mol Cell Biol
  doi: 10.1038/nrm1074
– volume: 80
  start-page: 159
  year: 2018
  end-page: 188
  ident: CR38
  article-title: Neuromuscular junction formation, aging, and disorders
  publication-title: Annu Rev Physiol
  doi: 10.1146/annurev-physiol-022516-034255
– volume: 12
  start-page: 1173
  year: 1994
  end-page: 1180
  ident: CR8
  article-title: Identification and purification of an agrin receptor from Torpedo postsynaptic membranes: a heteromeric complex related to the dystroglycans
  publication-title: Neuron
  doi: 10.1016/0896-6273(94)90324-7
– volume: 239
  start-page: 16
  year: 2010
  end-page: 33
  ident: CR29
  article-title: Crosstalk between Wnt and bone morphogenic protein signalling: a turbulent relationship
  publication-title: Dev Dyn
  doi: 10.1002/dvdy.22009
– volume: 34
  start-page: 367
  year: 2016
  end-page: 379
  ident: CR37
  article-title: An overview of osteocalcin progress
  publication-title: J Bone Miner Metab
  doi: 10.1007/s00774-015-0734-7
– volume: 5
  year: 2013
  ident: CR11
  article-title: The role of MuSK in synapse formation and neuromuscular disease
  publication-title: Cold Spring Harb Perspect Biol
  doi: 10.1101/cshperspect.a009167
– volume: 143
  start-page: 420
  year: 1990
  end-page: 430
  ident: CR45
  article-title: Progressive development of the rat osteoblast phenotype in vitro: reciprocal relationships in expression of genes associated with osteoblast proliferation and differentiation during formation of the bone extracellular matrix
  publication-title: J Cell Physiol
  doi: 10.1002/jcp.1041430304
– volume: 55
  start-page: 407
  year: 1990
  end-page: 418
  ident: CR42
  article-title: The agrin hypothesis
  publication-title: Cold Spring Harb Symp Quant Biol
  doi: 10.1101/sqb.1990.055.01.041
– volume: 11
  start-page: 212
  year: 2020
  ident: CR36
  article-title: Autoantibody specificities in : implications for improved diagnostics and therapeutics
  publication-title: Front Immunol
  doi: 10.3389/fimmu.2020.00212
– volume: 36
  start-page: 27
  year: 1987
  end-page: 48
  ident: CR10
  article-title: Bone structure and function
  publication-title: Instr Course Lect
– volume: 105
  start-page: 2471
  year: 1987
  end-page: 2478
  ident: CR44
  article-title: Identification of agrin, a synaptic organizing protein from Torpedo electric organ
  publication-title: J Cell Biol
  doi: 10.1083/jcb.105.6.2471
– volume: 92
  start-page: 41
  year: 2016
  end-page: 51
  ident: CR2
  article-title: Key transcription factors in the differentiation of mesenchymal stem cells
  publication-title: Differentiation
  doi: 10.1016/j.diff.2016.02.005
– volume: 119
  start-page: 199
  year: 2006
  end-page: 207
  ident: CR4
  article-title: Dystroglycan: from biosynthesis to pathogenesis of human disease
  publication-title: J Cell Sci
  doi: 10.1242/jcs.02814
– ident: CR32
– volume: 12
  start-page: eaax9086
  issue: 559
  year: 2020
  ident: CR18
  article-title: Agrin induces long-term osteochondral regeneration by supporting repair morphogenesis
  publication-title: Sci Transl Med
  doi: 10.1126/scitranslmed.aax9086
– volume: 6
  start-page: 156
  year: 2020
  ident: CR24
  article-title: Dissecting the extracellular complexity of neuromuscular junction organizers
  publication-title: Front Mol Biosci
  doi: 10.3389/fmolb.2019.00156
– ident: CR26
– volume: 23
  start-page: 1322
  year: 2016
  end-page: 1330
  ident: CR3
  article-title: Identification of a novel agrin-dependent pathway in cell signalling and adhesion within the erythroid niche
  publication-title: Cell Death Differ
  doi: 10.1038/cdd.2016.10
– volume: 144
  start-page: 2824
  year: 2017
  end-page: 2836
  ident: CR1
  article-title: Multiple modes of Lrp4 function in modulation of Wnt/β-catenin signalling during tooth development
  publication-title: Development
  doi: 10.1242/dev.150680
– volume: 292
  start-page: 1681
  year: 2001
  end-page: 1686
  ident: CR33
  article-title: Physiological regulation of the immunological synapse by agrin
  publication-title: Science
  doi: 10.1126/science.1056594
– volume: 6
  start-page: 6184
  year: 2015
  ident: CR12
  article-title: An oncogenic role of Agrin in regulating focal adhesion integrity in hepatocellular carcinoma
  publication-title: Nat Commun
  doi: 10.1038/ncomms7184
– volume: 16
  start-page: 32
  year: 2009
  end-page: 48
  ident: CR22
  article-title: Physiology of bone
  publication-title: Endocr Dev
  doi: 10.1159/000223687
– volume: 74
  start-page: 1649
  year: 2017
  end-page: 1657
  ident: CR31
  article-title: Wnt signalling and cellular metabolism in osteoblasts
  publication-title: Cell Mol Life Sci
  doi: 10.1007/s00018-016-2425-5
– volume: 23
  start-page: 268
  year: 2017
  end-page: 280
  ident: CR7
  article-title: Osteoblast differentiation and bone matrix formation in vivo and in vitro
  publication-title: Tissue Eng Part B Rev
  doi: 10.1089/ten.TEB.2016.0454
– volume: 127
  start-page: 363
  year: 2007
  end-page: 374
  ident: CR25
  article-title: Agrin is highly expressed by chondrocytes and is required for normal growth
  publication-title: Histochem Cell
  doi: 10.1007/s00418-006-0258-2
– volume: 37
  start-page: e00253
  year: 2017
  end-page: e316
  ident: CR30
  article-title: Bmp induces osteoblast differentiation through both Smad4 and mTORC1 signalling
  publication-title: Mol Cell Biol
  doi: 10.1128/MCB.00253-16
– volume: 3
  year: 2007
  ident: CR9
  article-title: Posttranscriptional expression regulation: what determines translation rates?
  publication-title: PLoS Comput Biol
  doi: 10.1371/journal.pcbi.0030057
– volume: 20
  start-page: 50
  year: 2006
  end-page: 58
  ident: CR49
  article-title: Agrin is involved in lymphocytes activation that is mediated by alpha-dystroglycan
  publication-title: FASEB J
  doi: 10.1096/fj.04-3303com
– volume: 3
  start-page: 15005
  year: 2015
  ident: CR46
  article-title: TGF-β/BMP signalling and other molecular events: regulation of osteoblastogenesis and bone formation
  publication-title: Bone Res
  doi: 10.1038/boneres.2015.5
– volume: 135
  start-page: 334
  year: 2008
  end-page: 342
  ident: CR34
  article-title: Lrp4 is a receptor for Agrin and forms a complex with MuSK
  publication-title: Cell
  doi: 10.1016/j.cell.2008.10.002
– volume: 99
  start-page: 1233
  year: 2006
  end-page: 1239
  ident: CR35
  article-title: Regulation of osteoblast differentiation by transcription factors
  publication-title: J Cell Biochem
  doi: 10.1002/jcb.20958
– ident: CR20
– volume: 165
  start-page: 535
  year: 2016
  end-page: 550
  ident: CR39
  article-title: On the dependency of cellular protein levels on mRNA abundance
  publication-title: Cell
  doi: 10.1016/j.cell.2016.03.014
– volume: 36
  start-page: S28
  year: 2005
  end-page: S33
  ident: CR15
  article-title: Discovery and development of BMPs
  publication-title: Injury
  doi: 10.1016/j.injury.2005.07.031
– volume: 52
  start-page: 45
  year: 2013
  end-page: 156
  ident: CR50
  article-title: Wnt/β-catenin signalling activates bone morphogenetic protein 2 expression in osteoblasts
  publication-title: Bone
  doi: 10.1016/j.bone.2012.09.029
– volume: 74
  start-page: 1649
  year: 2017
  ident: 3494_CR31
  publication-title: Cell Mol Life Sci
  doi: 10.1007/s00018-016-2425-5
– volume: 108
  start-page: 17
  year: 2002
  ident: 3494_CR43
  publication-title: Cell
  doi: 10.1016/s0092-8674(01)00622-5
– volume: 12
  start-page: 1173
  year: 1994
  ident: 3494_CR8
  publication-title: Neuron
  doi: 10.1016/0896-6273(94)90324-7
– volume: 99
  start-page: 1233
  year: 2006
  ident: 3494_CR35
  publication-title: J Cell Biochem
  doi: 10.1002/jcb.20958
– volume: 6
  start-page: 6184
  year: 2015
  ident: 3494_CR12
  publication-title: Nat Commun
  doi: 10.1038/ncomms7184
– volume: 127
  start-page: 363
  year: 2007
  ident: 3494_CR25
  publication-title: Histochem Cell
  doi: 10.1007/s00418-006-0258-2
– volume: 36
  start-page: 27
  year: 1987
  ident: 3494_CR10
  publication-title: Instr Course Lect
– volume: 55
  start-page: 407
  year: 1990
  ident: 3494_CR42
  publication-title: Cold Spring Harb Symp Quant Biol
  doi: 10.1101/sqb.1990.055.01.041
– volume: 23
  start-page: 1322
  year: 2016
  ident: 3494_CR3
  publication-title: Cell Death Differ
  doi: 10.1038/cdd.2016.10
– volume: 144
  start-page: 2824
  year: 2017
  ident: 3494_CR1
  publication-title: Development
  doi: 10.1242/dev.150680
– volume: 36
  start-page: S28
  year: 2005
  ident: 3494_CR15
  publication-title: Injury
  doi: 10.1016/j.injury.2005.07.031
– volume: 75
  start-page: 1228
  year: 2016
  ident: 3494_CR17
  publication-title: Ann Rheum Dis
  doi: 10.1136/annrheumdis-2015-207316
– volume: 15
  start-page: 1334
  year: 2009
  ident: 3494_CR21
  publication-title: Curr Pharm Des
  doi: 10.2174/138161209787846739
– volume: 37
  start-page: e00253
  year: 2017
  ident: 3494_CR30
  publication-title: Mol Cell Biol
  doi: 10.1128/MCB.00253-16
– volume: 143
  start-page: 420
  year: 1990
  ident: 3494_CR45
  publication-title: J Cell Physiol
  doi: 10.1002/jcp.1041430304
– volume: 25
  start-page: 402
  year: 2001
  ident: 3494_CR40
  publication-title: Methods
  doi: 10.1006/meth.2001.1262
– volume: 3
  year: 2007
  ident: 3494_CR9
  publication-title: PLoS Comput Biol
  doi: 10.1371/journal.pcbi.0030057
– volume: 6
  start-page: 18
  year: 2019
  ident: 3494_CR14
  publication-title: Front Mol Biosci
  doi: 10.3389/fmolb.2019.00018
– ident: 3494_CR26
  doi: 10.1007/978-1-61779-415-5
– volume: 12
  start-page: eaax9086
  issue: 559
  year: 2020
  ident: 3494_CR18
  publication-title: Sci Transl Med
  doi: 10.1126/scitranslmed.aax9086
– volume: 6
  start-page: 170
  year: 2019
  ident: 3494_CR28
  publication-title: Front Cell Dev Biol
  doi: 10.3389/fcell.2018.00170
– volume: 34
  start-page: 367
  year: 2016
  ident: 3494_CR37
  publication-title: J Bone Miner Metab
  doi: 10.1007/s00774-015-0734-7
– volume: 105
  start-page: 2471
  year: 1987
  ident: 3494_CR44
  publication-title: J Cell Biol
  doi: 10.1083/jcb.105.6.2471
– volume: 92
  start-page: 41
  year: 2016
  ident: 3494_CR2
  publication-title: Differentiation
  doi: 10.1016/j.diff.2016.02.005
– volume: 23
  start-page: 73
  year: 2016
  ident: 3494_CR19
  publication-title: Cancer Gene Ther
  doi: 10.1038/cgt.2016.4
– volume: 5
  year: 2013
  ident: 3494_CR11
  publication-title: Cold Spring Harb Perspect Biol
  doi: 10.1101/cshperspect.a009167
– volume: 239
  start-page: 16
  year: 2010
  ident: 3494_CR29
  publication-title: Dev Dyn
  doi: 10.1002/dvdy.22009
– volume: 119
  start-page: 199
  year: 2006
  ident: 3494_CR4
  publication-title: J Cell Sci
  doi: 10.1242/jcs.02814
– volume: 135
  start-page: 334
  year: 2008
  ident: 3494_CR34
  publication-title: Cell
  doi: 10.1016/j.cell.2008.10.002
– ident: 3494_CR20
  doi: 10.21769/BioProtoc.3534
– ident: 3494_CR32
  doi: 10.1371/journal.pone.0115004
– volume: 80
  start-page: 159
  year: 2018
  ident: 3494_CR38
  publication-title: Annu Rev Physiol
  doi: 10.1146/annurev-physiol-022516-034255
– volume: 13
  start-page: 227
  year: 2012
  ident: 3494_CR48
  publication-title: Nat Rev Genet
  doi: 10.1038/nrg3185
– volume: 292
  start-page: 1681
  year: 2001
  ident: 3494_CR33
  publication-title: Science
  doi: 10.1126/science.1056594
– volume: 20
  start-page: 50
  year: 2006
  ident: 3494_CR49
  publication-title: FASEB J
  doi: 10.1096/fj.04-3303com
– volume: 11
  start-page: 212
  year: 2020
  ident: 3494_CR36
  publication-title: Front Immunol
  doi: 10.3389/fimmu.2020.00212
– volume: 40
  start-page: 59
  year: 2018
  ident: 3494_CR23
  publication-title: Curr Opin Pharmacol
  doi: 10.1016/j.coph.2018.03.005
– volume: 165
  start-page: 535
  year: 2016
  ident: 3494_CR39
  publication-title: Cell
  doi: 10.1016/j.cell.2016.03.014
– volume: 61
  start-page: 848
  year: 2012
  ident: 3494_CR13
  publication-title: Neurochem Int
  doi: 10.1016/j.neuint.2012.02.028
– volume: 12
  start-page: 2151
  year: 2018
  ident: 3494_CR16
  publication-title: J Tissue Eng Regen Med
  doi: 10.1002/term.2738
– volume: 547
  start-page: 179
  year: 2017
  ident: 3494_CR5
  publication-title: Nature
  doi: 10.1038/nature22978
– volume: 6
  start-page: 156
  year: 2020
  ident: 3494_CR24
  publication-title: Front Mol Biosci
  doi: 10.3389/fmolb.2019.00156
– volume: 52
  start-page: 45
  year: 2013
  ident: 3494_CR50
  publication-title: Bone
  doi: 10.1016/j.bone.2012.09.029
– volume: 16
  start-page: 32
  year: 2009
  ident: 3494_CR22
  publication-title: Endocr Dev
  doi: 10.1159/000223687
– volume: 716
  year: 2020
  ident: 3494_CR27
  publication-title: Neurosci Lett
  doi: 10.1016/j.neulet.2019.134676
– volume: 23
  start-page: 268
  year: 2017
  ident: 3494_CR7
  publication-title: Tissue Eng Part B Rev
  doi: 10.1089/ten.TEB.2016.0454
– volume: 3
  start-page: 15005
  year: 2015
  ident: 3494_CR46
  publication-title: Bone Res
  doi: 10.1038/boneres.2015.5
– volume: 4
  start-page: 295
  year: 2003
  ident: 3494_CR6
  publication-title: Nat Rev Mol Cell Biol
  doi: 10.1038/nrm1074
– volume: 118
  start-page: 2733
  year: 2011
  ident: 3494_CR41
  publication-title: Blood
  doi: 10.1182/blood-2011-01-331272
– volume: 80
  start-page: 101
  year: 2015
  ident: 3494_CR47
  publication-title: Bone
  doi: 10.1016/j.bone.2015.05.012
SSID ssj0014669
Score 2.399022
Snippet The extracellular matrix protein Agrin has been detected in chondrocytes and endosteal osteoblasts but its function in osteoblast differentiation has not been...
SourceID proquest
gale
pubmed
crossref
springer
SourceType Aggregation Database
Index Database
Enrichment Source
Publisher
StartPage 335
SubjectTerms 3T3 Cells
Agrin
Agrin - analysis
Agrin - genetics
Animals
Biomedical and Life Sciences
Biomedicine
Bone morphogenetic proteins
Cell Differentiation
Cells, Cultured
Chondrocytes
Chondrogenesis
Dystroglycan
Extracellular matrix
Gene Expression Regulation
Genes
Human Genetics
Humans
low density lipoprotein
Low density lipoproteins
Matrix protein
Mice
Mice, Inbred C57BL
Molecular Medicine
Osteoblastogenesis
Osteoblasts
Osteoblasts - cytology
Osteoblasts - metabolism
Osteogenesis
Proteins
Proteomics
Receptor density
Regular Article
Signal transduction
therapeutics
Wnt protein
SummonAdditionalLinks – databaseName: Health & Medical Collection
  dbid: 7X7
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3dS90wFD9sjsFeRDd19TPCwIet7LZJ0_ZJLqLIYHtSuG8hTXJF5lq1FfS_95w0rbuC97knJcn5yEnOxw_gW8b1PNPOxgbPqljk1sSl5TouTSJRpgtdefSG33_k-aX4Nctm4cGtDWmVg030hto2ht7If6YZDRW5yI5v72JCjaLoaoDQeA8ffOsylOd8Nl640Ah4SDuUY_QipZyFohlfOkeRTLxIp5RQJEoRlwsH02vz_N_59Cpg6s-hszVYDQ4km_YcX4d3rv4MH3tIyacv8Bf5ztDg3mt6kqccU_aPuvA_Mt-R4bpm0yv8MbtukconwTrLqidGtR5Nha501zJdW-ZT2AkLy7Wsa5iPJ7ABTaXr-bkBl2enFyfncQBUQE5MZBdLjy4ireN47xU6MYVLMpcJnXN0fIQhZ81RB7sy57qSLrXSoAuAV7J0ok2e8k1YqZvafQVW2Yprm2jq2CZsUWlZzCWFAMV8zq0oIkiG3VQmdBsn0IsbNfZJ9hxQyAHlOaDKCL6PY277XhtLqQ-ISaqvFx0VVU0JTEQQtFoER56CVJW2XYeKA1wBNb1aoNxdoEQVM4ufB0FQQcVb9SKQERyOn2kkpa3VrnkgGsL4wl1Jl9BILnP0o8pJBFu9kI2r5wK9tzLHtf4YpO5lAm9vzfby-e7Ap5QE31dU7sJKd__g9tC16qp9rz_PS5gcKA
  priority: 102
  providerName: ProQuest
Title The extracellular matrix protein Agrin is expressed by osteoblasts and contributes to their differentiation
URI https://link.springer.com/article/10.1007/s00441-021-03494-9
https://www.ncbi.nlm.nih.gov/pubmed/34223979
https://www.proquest.com/docview/2588784745
https://www.proquest.com/docview/2548607292
https://www.proquest.com/docview/2636724190
Volume 386
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV1ba9RAFD7YLYIv3i_RWkcQfNCUTWYyyTxG2VoUi4gL61OY25ZSzUqTBeuv95zJRXfRQp8WNie7mTPnzHyTc_kAXmRcLzPtXWxxr4pF7mysHNexsolEmy60CewNH4_l0Vy8X2SLviisGbLdh5BkWKnHYjeKPeLRN6UUIKFErHZgN0sKVUxgt3z39cNsjB4IGajs0H4RPUq56Itl_v0rGxvS9rL81760FSgN-8_hLZgPT96lnZwdrFtzYH9tNXW86tBuw80ekLKys6A7cM3Xd-F6R1F5cQ_O0I4YLuDnml7xU84q-05d_X-y0OHhtGblCf4XO21QKiTVesfMBaPakZVBaN42TNeOhZR44tbyDWtXLMQn2MDO0nb2cR_mh7Mvb4_inqABZ3Yq21gGthLpPMdztNCJLXyS-UzonCOQEpbAn6eOeCrn2kifOmkRUuARL51qm6f8AUzqVe0fATPOcO0STR3ghCuMlsVSUkhRLJfciSKCZJilyvbdy4lE41s19l0OSqxQiVVQYqUieDXe86Pr3XGp9DOa_KqrPx0dvyqJnEQQVVsEL4MEuT6pXfcVDDgCaqK1Ibm3IYkuazcvDwZW9UtGU6UZ-YbIRRbB8_Ey3UlpcLVfrUmGOMNQK-klMpLLHHGZmkbwsDPecfRcIBpUOY719WCIfx7g_6p5fDXxJ3AjJVsOFZt7MGnP1_4pQrfW7MNOvsj3e3_Fzzez40-f8dt5Wv4GENg5Vg
linkProvider Springer Nature
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1Lb9QwEB5VWyG4oPIOLdRIIA4QsUkcJzkgtECrLW1XCLXS3lzH9qKqJWmbVGX_FL-RGedRthJ76zljy_aMZ8aZxwfwOo7ULFbW-Bptlc8To_3MRMrPdCBQplOVO_SG_YkYH_Jv03i6An-6WhhKq-x0olPUptT0j_xDGNNQnvD409m5T6hRFF3tIDQasdi18yt8slUfd74if9-E4fbWwZex36IK4HKGovaFg9gQxkb4-OMq0KkNYhtzlURo_bkmj8VSG7csiVQubGiERjuI75JwqHRCjQ5Q5a9yqmgdwOrnrcn3H33cAifOmrgF-q1CTNsyHVesR7FTfLqHlMLEM-5nC6bwpkH4xyLeCNE6y7e9Bvdbl5WNGhl7ACu2eAh3GhDL-SM4QUljqOIvFAUBKKuV_aK-_7-Z6wFxXLDRT5yYHVdI5dJurWH5nFF1SZmj815XTBWGuaR5Qt-yFatL5iIYrMNvqRsJegyHt3LYT2BQlIV9Biw3eaRMoKhHHDdprkQ6ExR05LNZZHjqQdCdptRtf3OC2TiVfWdmxwGJHJCOAzLz4F0_5qzp7rGUepOYJJsK1V41yBHBl3ACc_PgraMg5UDHrtoaB9wBtdlaoNxYoMRLrRc_d4IgW6VSyesr4MGr_jONpES5wpaXREOoYngq4RIaEYkEPbds6MHTRsj63Ucc_cUswb2-76TuegH_P5rny9e7CXfHB_t7cm9nsrsO90K6BK6ecwMG9cWlfYGOXZ2_bG8Tg6PbvsB_AUH1V1s
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1Lb9QwEB5VRSAuFW8ChRoJxAGibmLHSQ4IrSirlkLFgUp7M47toKqQlCYV3b_WX8eM8yhbib31nPHKj3nuPD6AlwnXZaKdDQ3aqlCk1oS55TrMTSSRpzNdePSGLwdy91B8mifzNbgYemGorHLQiV5R29rQf-TbcUJLRSqS7bIvi_i6M3t_8jskBCnKtA5wGh2L7LvFHwzfmnd7O_jWr-J49vHbh92wRxjArU1kG0oPtyGt4xgICh2ZzEWJS4ROOXoCwpD34mikW55yXUgXW2nQJmKMEk-0SWnoAar_G0g9ocAvnY_BHiogD6eHMoQerJTzvmHHt-1RFhWD-JiKmUQuwnzJKF41Df_YxivJWm8DZ3dgo3de2bTjtruw5qp7cLODs1zch2PkOYY3dqopHUD1rewXIQCcMz8N4qhi0x_4w-yoQSpfgOssKxaM-kzqAt34tmG6ssyXzxMOl2tYWzOfy2ADkkvb8dIDOLyWq34I61VducfACltwbSNN0-KEzQots1JS-lGUJbciCyAablOZftI5AW78VOOMZv8CCl9A-RdQeQBvxjUn3ZyPldRb9Eiq61UdlYSaEpCJIFi3AF57ClITdO2673bAE9DArSXKzSVKFG-z_HlgBNWrl0ZdCkMAL8bPtJJK5ipXnxEN4YvhrcQraCSXKfpw-SSARx2TjafnAj3HPMWzvh247nID_7-aJ6v3uwW3UGzV572D_adwOyYZ8I2dm7Denp65Z-jhtcVzL0oMvl-37P4FstdaIg
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=The+extracellular+matrix+protein+Agrin+is+expressed+by+osteoblasts+and+contributes+to+their+differentiation&rft.jtitle=Cell+and+tissue+research&rft.au=Souza%2C+Alann+Thaffarell+Portilho&rft.au=Lopes%2C+Helena+Bacha&rft.au=Oliveira%2C+Fabiola+Singaretti&rft.au=Weffort%2C+Denise&rft.date=2021-11-01&rft.eissn=1432-0878&rft.volume=386&rft.issue=2&rft.spage=335&rft_id=info:doi/10.1007%2Fs00441-021-03494-9&rft_id=info%3Apmid%2F34223979&rft.externalDocID=34223979
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0302-766X&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0302-766X&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0302-766X&client=summon