A Microfluidic Device for Culturing an Encapsulated Ovarian Follicle

Microfluidic chips have been proved effective in mimicking different organs of human body. Simulating human ovarian follicles by microfluidic device will be useful in exploring the mechanism of folliculogenesis and related diseases. In this paper, a microfluidic chip was designed to culture a single...

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Published inMicromachines (Basel) Vol. 8; no. 11; p. 335
Main Authors Aziz, Aziz Ur Rehman, Fu, Mengjie, Deng, Jiu, Geng, Chunyang, Luo, Yong, Lin, Bingcheng, Yu, Xiaohui, Liu, Bo
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 20.11.2017
MDPI
Subjects
Beads
Calcium alginate
Encapsulation
Estrogens
Genotype & phenotype
Hydrogels
microfluidics
organ on a chip
Organs
ovarian follicles
Sex hormones
organ on a chip
ovarian follicles
microfluidics
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Abstract Microfluidic chips have been proved effective in mimicking different organs of human body. Simulating human ovarian follicles by microfluidic device will be useful in exploring the mechanism of folliculogenesis and related diseases. In this paper, a microfluidic chip was designed to culture a single human pre-antral follicle. Ovarian follicles were first encapsulated in 3D calcium alginate hydrogel beads and then cultured on chip and in dish under same conditions. The diameters of cultured ovarian follicles were measured, and the same amount of medium was collected from microfluidic device or dish per two days for measuring the estradiol and androgen concentrations. The results confirmed the successful growth of ovarian follicles on chip with their hormonal trends and diameters increase, which were similar to ovarian follicles cultured in dish. It is concluded that this microfluidic chip can be used to culture a single human ovarian follicle, which provides a useful tool to explore the hormonal changes and their interactions during folliculogenesis.
AbstractList Microfluidic chips have been proved effective in mimicking different organs of human body. Simulating human ovarian follicles by microfluidic device will be useful in exploring the mechanism of folliculogenesis and related diseases. In this paper, a microfluidic chip was designed to culture a single human pre-antral follicle. Ovarian follicles were first encapsulated in 3D calcium alginate hydrogel beads and then cultured on chip and in dish under same conditions. The diameters of cultured ovarian follicles were measured, and the same amount of medium was collected from microfluidic device or dish per two days for measuring the estradiol and androgen concentrations. The results confirmed the successful growth of ovarian follicles on chip with their hormonal trends and diameters increase, which were similar to ovarian follicles cultured in dish. It is concluded that this microfluidic chip can be used to culture a single human ovarian follicle, which provides a useful tool to explore the hormonal changes and their interactions during folliculogenesis.
Author Aziz, Aziz Ur Rehman
Liu, Bo
Geng, Chunyang
Fu, Mengjie
Deng, Jiu
Luo, Yong
Yu, Xiaohui
Lin, Bingcheng
AuthorAffiliation 1 Department of Biomedical Engineering, Dalian University of Technology, Dalian 116024, China; azizjatoi@hotmail.com (A.U.R.A.); 1352257919@mail.dlut.edu.cn (C.G.)
3 School of Pharmaceutical Science and Technology, Dalian University of Technology, Dalian 116024, China; dengjiu@mail.dlut.edu.cn (J.D.); yluo@dlut.edu.cn (Y.L.); bclin@dicp.ac.cn (Bi.L.)
2 Dalian Institute of Maternal and Child Health Care, Dalian 116024, China; fmjnewlife@126.com
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– name: 2 Dalian Institute of Maternal and Child Health Care, Dalian 116024, China; fmjnewlife@126.com
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Keywords organ on a chip
ovarian follicles
microfluidics
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Snippet Microfluidic chips have been proved effective in mimicking different organs of human body. Simulating human ovarian follicles by microfluidic device will be...
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SubjectTerms Beads
Calcium alginate
Encapsulation
Estrogens
Genotype & phenotype
Hydrogels
microfluidics
organ on a chip
Organs
ovarian follicles
Sex hormones
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Title A Microfluidic Device for Culturing an Encapsulated Ovarian Follicle
URI https://www.ncbi.nlm.nih.gov/pubmed/30400524
https://www.proquest.com/docview/1977893423
https://search.proquest.com/docview/2130804171
https://pubmed.ncbi.nlm.nih.gov/PMC6190016
https://doaj.org/article/d293414ce0ad4037885d08dc4871787f
Volume 8
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