Glycomic analysis of gastric carcinoma cells discloses glycans as modulators of RON receptor tyrosine kinase activation in cancer

• Published in: Biochimica et biophysica acta Vol. 1860; no. 8; pp. 1795 - 1808
• Main Authors: Mereiter, Stefan, Magalhães, Ana, Adamczyk, Barbara, Jin, Chunsheng, Almeida, Andreia, Drici, Lylia, Ibáñez-Vea, Maria, Gomes, Catarina, Ferreira, José A., Afonso, Luis P., Santos, Lúcio L., Larsen, Martin R., Kolarich, Daniel, Karlsson, Niclas G., Reis, Celso A.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.08.2016
• Subjects: Biochemistry & Molecular Biology; Biofysik; Biophysics; biosynthesis; breast-cancer; c-met; colorectal-cancer; epitopes; Gastric cancer; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Neoplastic; gene overexpression; Glycome; Glycomics; glycosylation; Humans; in-vivo; insulin receptors; integrins; invasive growth; Lewis X Antigen - biosynthesis; Lewis X Antigen - genetics; linked; liquid chromatography; mass spectrometry; medicine; Molecular Biology; Molekylärbiologi; n-acetylglucosaminyltransferase-iii; neoplasm cells; Neoplasm Proteins - biosynthesis; Neoplasm Proteins - genetics; next-generation; oligosaccharides; patients; personalized medicine; phenotype; polysaccharides; Polysaccharides - biosynthesis; Polysaccharides - genetics; Receptor Protein-Tyrosine Kinases - genetics; Receptor Protein-Tyrosine Kinases - metabolism; RON; Sialome; Sialyl Lewis X (SLeX); Sialyltransferases - biosynthesis; Sialyltransferases - genetics; ST3GAL4; stomach neoplasms; Stomach Neoplasms - genetics; Stomach Neoplasms - metabolism; structural-analysis; HILIC-FLD-UPLC; PLA; IHC; Glycome; PTM; RTK; SLeX; RON; Sialyl Lewis X (SLeX); SLea; ST3GAL4; GC; Gastric cancer; Sialome

Terminal α2-3 and α2-6 sialylation of glycans precludes further chain elongation, leading to the biosynthesis of cancer relevant epitopes such as sialyl-Lewis X (SLeX). SLeX overexpression is associated with tumor aggressive phenotype and patients' poor prognosis. MKN45 gastric carcinoma cells...