Photoaffinity labeling and fatty acid permeation in 3T3-L1 adipocytes

• Published in: The Journal of biological chemistry Vol. 266; no. 33; pp. 22621 - 22625
• Main Authors: TRIGATTI, B. L, MANGROO, D, GERBER, G. E
• Format: Journal Article
• Language: English
• Published: Bethesda, MD American Society for Biochemistry and Molecular Biology 25.11.1991
• Subjects: 3T3 Cells; Adipose Tissue - metabolism; Analytical, structural and metabolic biochemistry; Animals; Azirines - metabolism; Binding and carrier proteins; Biological and medical sciences; Biological Transport; Carrier Proteins - isolation & purification; Carrier Proteins - metabolism; Cell Differentiation; Cell Membrane - metabolism; cell membranes; Fatty Acid-Binding Protein 7; Fatty Acid-Binding Proteins; fatty acids; Fatty Acids, Nonesterified - metabolism; Fundamental and applied biological sciences. Psychology; Kinetics; Mice; Molecular Weight; Neoplasm Proteins; Nerve Tissue Proteins; Oleic Acid; Oleic Acids - metabolism; Proteins; Characterization; Adipocyte; Binding protein; Oleic acid; Plasma membrane; 3T3-Cell line; Photoaffinity labelling; Cell differentiation; Fatty acids

Long chain fatty acid uptake was investigated in 3T3-L1 cells. Differentiation of these cells from fibroblasts to adipocytes was accompanied by an 8.5-fold increase in the rate of oleate uptake. This was saturable in adipocytes with apparent Kt and Vmax values of 78 nM and 16 nmol/min/mg cell protei...