A high-throughput-compatible assay to measure the degradation of endogenous Huntingtin proteins

• Published in: Acta pharmacologica Sinica Vol. 37; no. 10; pp. 1307 - 1314
• Main Authors: Wu, Peng, Lu, Ming-xing, Cui, Xiao-tian, Yang, He-qing, Yu, Shen-liang, Zhu, Jian-bin, Sun, Xiao-li, Lu, Boxun
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.09.2016; Nature Publishing Group
• Subjects: Animals; Biomedical and Life Sciences; Biomedicine; Cell Line; Click Chemistry; High-Throughput Screening Assays; Humans; Huntingtin Protein - metabolism; Immunology; Internal Medicine; Medical Microbiology; Mice; Original; original-article; Pharmacology/Toxicology; Proteolysis; Vaccine; SPAAC; pulse-chase; high-throughput; click chemistry; Huntington's disease; protein degradation; PolyQ

Aim： The accumulation of disease-causing proteins is a common hallmark of many neurodegenerative disorders. Measuring the degradation of such proteins using high-throughput-compatible assays is highly desired for the identification of genetic and chemica modulators of degradation. For example, Hunti...