Immunogenicity of intrathecal plasmid gene delivery: cytokine release and effects on transgene expression

• Published in: The journal of gene medicine Vol. 11; no. 9; pp. 782 - 790
• Main Authors: Hughes, Travis S., Langer, Stephen J., Virtanen, Salla I., Chavez, Raymond A., Watkins, Linda R., Milligan, Erin D., Leinwand, Leslie A.
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.09.2009; Wiley Periodicals Inc
• Subjects: Animal models; Animals; Cell Line; Central nervous system; Central pattern generator; Cerebrospinal fluid; CpG; CpG Islands - genetics; Cytokines; Cytokines - secretion; Data processing; Gene Expression; Gene therapy; Gene transfer; Genetic Therapy; Humans; Immune response; Immunogenicity; Inflammatory; Injections, Spinal; Interleukin 1; Intrathecal; lipopolysaccharide; Male; Plasmid; Plasmids; Rats; Rats, Sprague-Dawley; Spinal cord; Toll-Like Receptor 9 - genetics; Transfection; Transgenes

Background One method for the delivery of therapeutic proteins to the spinal cord is to inject nonviral gene vectors including plasmid DNA into the cerebrospinal fluid (CSF) that surrounds the spinal cord (intrathecal space). This approach has produced therapeutic benefits in animal models of diseas...