Analysis of human liver disease using a cluster of differentiation (CD) antibody microarray
Background A CD antibody microarray has been previously developed allowing semi‐quantitative identification of greater than 80 CD antigens on circulating leucocytes from peripheral blood samples. This assay, which uses a live cell‐capture technique, enables an extensive leucocyte immunophenotype det...
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Published in | Liver international Vol. 32; no. 10; pp. 1527 - 1534 |
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Main Authors | , , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Blackwell Publishing Ltd
01.11.2012
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Subjects | |
Online Access | Get full text |
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Summary: | Background
A CD antibody microarray has been previously developed allowing semi‐quantitative identification of greater than 80 CD antigens on circulating leucocytes from peripheral blood samples. This assay, which uses a live cell‐capture technique, enables an extensive leucocyte immunophenotype determination in a single analysis and to date this has been used successfully to characterise diseases including human leukaemias and HIV infection.
Aims
To determine CD antigen expression profiles for patients with various liver diseases and to look for preserved disease‐specific signatures.
Methods
Three liver disease groups including hepatitis C (HCV) (n = 35), non‐alcoholic steatohepatitis (NASH) (n = 21) and alcohol‐related liver disease (n = 14) were compared with a normal group (n = 23). Hierarchal Clustering (HCL) and Principal Component Analysis (PCA) of the data revealed distinct binding patterns for patients with and without cirrhosis.
Results
Patients with cirrhosis and portal hypertension compared with those without cirrhosis had significantly reduced expression of several markers of T‐cell function including CD45, CD8, CD28 and TCR α/β. Disease prediction algorithms based on the expression data were able to discriminate cirrhotics from non‐cirrhotics with 71% overall success, which improved to 77% when only patients with HCV were considered.
Conclusions
These results demonstrate disease‐specific consensus patterns of expression of CD antigens for patients with chronic liver disease, suggesting that the CD antibody array is a promising tool in the analysis of human liver disease, and with further refinement may have future research and clinical utility. |
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Bibliography: | istex:19A49D2040B1F7E4F0C9AB6B4ED40758F4F94C62 ark:/67375/WNG-KZ7TZWJX-Z ArticleID:LIV2854 Sylvia and Charles Viertel Foundation ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1 |
ISSN: | 1478-3223 1478-3231 |
DOI: | 10.1111/j.1478-3231.2012.02854.x |