High-resolution peptide separations using nano-LC at ultra-high pressure
We report on the optimization of nano‐LC gradient separations of proteomic samples that vary in complexity. The gradient performance limits were visualized by kinetic plots depicting the gradient time needed to achieve a certain peak capacity, while using the maximum system pressure of 80 MPa. The s...
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| Published in | Journal of separation science Vol. 36; no. 7; pp. 1192 - 1199 |
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| Main Authors | , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Weinheim
Blackwell Publishing Ltd
01.04.2013
Wiley Wiley Subscription Services, Inc |
| Subjects | |
| Online Access | Get full text |
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| Abstract | We report on the optimization of nano‐LC gradient separations of proteomic samples that vary in complexity. The gradient performance limits were visualized by kinetic plots depicting the gradient time needed to achieve a certain peak capacity, while using the maximum system pressure of 80 MPa. The selection of the optimal particle size/column length combination and corresponding gradient steepness was based on scouting the performance of 75 μm id capillary columns packed with 2, 3, and 5 μm fully porous silica C18 particles. At optimal gradient conditions, peak capacities up to 500 can be obtained within a 120 min gradient using 2 μm particle‐packed capillary columns. Separations of proteomic samples including a cytochrome c tryptic digest, a bovine serum albumin tryptic digest, a six protein mix digest, and an Escherichia coli digest are demonstrated while operating at the kinetic‐performance limit, i.e. using 2‐μm packed columns, adjusting the column length and scaling the gradient steepness according to sample complexity. Finally, good run‐to‐run retention time stability (RSD values below 0.18%) was demonstrated applying ultra‐high pressure conditions. |
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| AbstractList | We report on the optimization of nano‐
LC
gradient separations of proteomic samples that vary in complexity. The gradient performance limits were visualized by kinetic plots depicting the gradient time needed to achieve a certain peak capacity, while using the maximum system pressure of 80 MPa. The selection of the optimal particle size/column length combination and corresponding gradient steepness was based on scouting the performance of 75 μm id capillary columns packed with 2, 3, and 5 μm fully porous silica
C
18 particles. At optimal gradient conditions, peak capacities up to 500 can be obtained within a 120 min gradient using 2 μm particle‐packed capillary columns. Separations of proteomic samples including a cytochrome
c
tryptic digest, a bovine serum albumin tryptic digest, a six protein mix digest, and an
Escherichia
coli
digest are demonstrated while operating at the kinetic‐performance limit, i.e. using 2‐μm packed columns, adjusting the column length and scaling the gradient steepness according to sample complexity. Finally, good run‐to‐run retention time stability (
RSD
values below 0.18%) was demonstrated applying ultra‐high pressure conditions. We report on the optimization of nano-LC gradient separations of proteomic samples that vary in complexity. The gradient performance limits were visualized by kinetic plots depicting the gradient time needed to achieve a certain peak capacity, while using the maximum system pressure of 80 MPa. The selection of the optimal particle size/column length combination and corresponding gradient steepness was based on scouting the performance of 75 mu m id capillary columns packed with 2, 3, and 5 mu m fully porous silica C18 particles. At optimal gradient conditions, peak capacities up to 500 can be obtained within a 120 min gradient using 2 mu m particle-packed capillary columns. Separations of proteomic samples including a cytochrome c tryptic digest, a bovine serum albumin tryptic digest, a six protein mix digest, and an Escherichia coli digest are demonstrated while operating at the kinetic-performance limit, i.e. using 2- mu m packed columns, adjusting the column length and scaling the gradient steepness according to sample complexity. Finally, good run-to-run retention time stability (RSD values below 0.18%) was demonstrated applying ultra-high pressure conditions. We report on the optimization of nano-LC gradient separations of proteomic samples that vary in complexity. The gradient performance limits were visualized by kinetic plots depicting the gradient time needed to achieve a certain peak capacity, while using the maximum system pressure of 80 MPa. The selection of the optimal particle size/column length combination and corresponding gradient steepness was based on scouting the performance of 75 µm id capillary columns packed with 2, 3, and 5 µm fully porous silica C18 particles. At optimal gradient conditions, peak capacities up to 500 can be obtained within a 120 min gradient using 2 µm particle-packed capillary columns. Separations of proteomic samples including a cytochrome c tryptic digest, a bovine serum albumin tryptic digest, a six protein mix digest, and an Escherichia coli digest are demonstrated while operating at the kinetic-performance limit, i.e. using 2-µm packed columns, adjusting the column length and scaling the gradient steepness according to sample complexity. Finally, good run-to-run retention time stability (RSD values below 0.18%) was demonstrated applying ultra-high pressure conditions. [PUBLICATION ABSTRACT] We report on the optimization of nano-LC gradient separations of proteomic samples that vary in complexity. The gradient performance limits were visualized by kinetic plots depicting the gradient time needed to achieve a certain peak capacity, while using the maximum system pressure of 80 MPa. The selection of the optimal particle size/column length combination and corresponding gradient steepness was based on scouting the performance of 75 μm id capillary columns packed with 2, 3, and 5 μm fully porous silica C18 particles. At optimal gradient conditions, peak capacities up to 500 can be obtained within a 120 min gradient using 2 μm particle-packed capillary columns. Separations of proteomic samples including a cytochrome c tryptic digest, a bovine serum albumin tryptic digest, a six protein mix digest, and an Escherichia coli digest are demonstrated while operating at the kinetic-performance limit, i.e. using 2-μm packed columns, adjusting the column length and scaling the gradient steepness according to sample complexity. Finally, good run-to-run retention time stability (RSD values below 0.18%) was demonstrated applying ultra-high pressure conditions. |
| Author | Desmet, Gert Eeltink, Sebastiaan Nováková, Lucie De Pra, Mauro Vaast, Axel Stassen, Catherine Swart, Remco Broeckhoven, Ken |
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| CitedBy_id | crossref_primary_10_1016_j_aca_2014_09_006 crossref_primary_10_1016_j_chroma_2022_463251 crossref_primary_10_1002_jssc_201600775 crossref_primary_10_1016_j_aca_2019_08_068 crossref_primary_10_1016_j_chroma_2015_07_090 crossref_primary_10_1002_jssc_202100116 crossref_primary_10_1016_j_jprot_2017_06_013 crossref_primary_10_1007_s10337_018_3647_5 crossref_primary_10_4155_fsoa_2016_0014 crossref_primary_10_1002_jssc_201500765 crossref_primary_10_4155_bio_15_92 crossref_primary_10_56530_lcgc_eu_bm1469g6 crossref_primary_10_1016_j_chroma_2021_462310 crossref_primary_10_1021_acs_analchem_5b04093 |
| Cites_doi | 10.1016/S0021-9673(97)00376-2 10.1016/j.chroma.2009.02.075 10.1016/j.chroma.2011.07.089 10.1016/j.chroma.2004.10.107 10.1038/nprot.2012.036 10.1016/j.chroma.2005.11.112 10.1371/journal.pbio.1000048 10.1016/j.chroma.2010.02.023 10.1002/jssc.200800639 10.1016/j.chroma.2010.05.015 10.1016/j.aca.2012.04.010 10.1021/ac0202280 10.1016/j.chroma.2011.12.088 10.1002/jssc.201000023 10.1002/jssc.201000339 10.1016/j.chroma.2010.03.037 10.1021/ac60220a012 10.1016/S0021-9673(99)00765-7 10.1016/0021-9673(96)00319-6 10.1016/j.copbio.2010.09.002 10.1016/j.chroma.2006.07.090 10.1016/j.aca.2008.06.017 10.1016/j.chroma.2009.02.032 10.1016/j.chroma.2005.12.050 10.1021/ac048455k 10.1016/j.drudis.2009.02.007 10.1021/pr050205c 10.1016/j.chroma.2007.02.016 10.1016/1044-0305(94)87010-1 10.1021/ac9001244 10.1007/s00216-009-3305-8 |
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| Keywords | Capillary column Ultra-high pressures Elution Gradient Peak resolution Chemical analysis Flow rate Nano flow rate Peptides HPLC chromatography Kinetic method Kinetic plot Packed capillary columns Protein Operating conditions Column coupling Reversed phase chromatography Ultraviolet detector Pressure effect Analysis method Plate efficiency Peptide fragment Proteomics Packed column |
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| References | Wu, Q., Yuan, H., Zhang, L., Zhang, Y., Anal. Chim. Acta 2012, 731, 1-10. Horie, K., Sato, Y., Kimura, T., Nakamura, T., Ishihama, Y. Oda, Y., Ikegami, T., Tanaka, N., J. Chromatogr. A 2012, 1228, 283-291. Ruta, J., Guillarme, D., Rudaz, S., Veuthey, J.-L., J. Sep. Sci. 2010, 33, 2465-2477. Wang, X., Barber, W. E., Carr, P. W., J. Chromatogr. A 2006, 1107, 139-151. Beck, M., Claassen, M., Aebersold, R., Curr. Opin. Biotechnol. 2011, 22, 3-8. Eeltink, S., Decrop, W. M. C., Steiner, F., Ursem, M., Cabooter, D., Desmet, G., Kok, W. T., J. Sep. Sci. 2010, 33, 2629-2635. Poppe, H., J. Chromatogr. A 1997, 778, 3-21. Ishihama, Y., J. Chromatogr. A 2005, 1067, 73-83. Silva, J. C., Denny, R., Dorschel, C. A., Gorenstein, M., Kass, I. J., Li, G. Z., McKenna, T., Nold, M. J., Richardson, K., Young, P., Geromanos, S., Anal. Chem. 2005, 77, 2187-2200. Eeltink, S., Desmet, G., Vivó-Truyols, G., Rozing, G. P., Schoenmakers, P. J., Kok, W. T., J. Chromatogr. A 2006, 1104, 256-262. Broeckhoven, K., Cabooter, D., Lynen, F., Sandra, P., Desmet, G., J. Chromatogr. A 2010, 1217, 2787-2795. Vaast, A., Broeckhoven, K., Dolman, S., Desmet, G., Eeltink, S., J. Chromatogr. A 2012, 1228, 270-275. Carr, P. W., Wang, X. L., Stoll, D. R., Anal. Chem. 2009, 81, 5342-5353. Chen, G., Pramanik, B. N., Drug Discovery Today 2009, 14, 465-471. Köcher, T., Pichler, P., Swart, R., Mechtler, K., Nat. Protoc. 2012, 7, 882-890. Chen, C.-H., Anal. Chim. Acta 2008, 624, 16-36. Eeltink, S., Dolman, S., Detobel, F., Swart, R., Ursem, M., Schoenmakers, P. J., J. Chromatogr. A 2010, 1217, 6610-6615. Dolan, J. W., Snyder, L. R., Djordjevic, N. M., Hill, D. W., Waeghe, T. J., J. Chromatogr. A 1999, 857, 1-20. Lanckmans, K., Van Eeckhaut, A., Sarre, S., Smolders, I., Michotte, Y., J. Chromatogr. A 2006, 1131, 166-175. Eeltink, S., Dolman, S., Swart, R., Ursem, M., Schoenmakers, P. J., J. Chromatogr. A 2009, 1216, 7368-7374. Banks, J. F. Jr, J. Chromatogr. 1996, 743, 99-104. Andren, P. E., Emmet, M. R., Caprioli, R. M., J. Am. Soc. Mass Spectrom. 1994, 5, 867-869. Giddings, J. C., Anal. Chem. 1965, 37, 60-63. Fairchild, J. N., Walworth, M. J., Horváth, K., Guiochon, G., J. Chromatogr. A 2010, 1217, 4779-4783. Shen, Y., Zhao, R., Berger, S. J., Anderson, G. A., Rodriguez, N., Smith, R. D., Anal. Chem. 2002, 74, 4235-4249. Donato, P., Dugo, P., Cacciola, F., Dugo, G., Mondello, L., J. Sep. Sci. 2009, 32, 1129-1136. Guillarme, D., Grata, E., Glauser, G., Wolfender, J. L., Veuthey, J.-L., Rudaz, S., J. Chromatogr. A 2009, 1216, 3232-3243. Guillarme, D., Ruta, J., Rudaz, S., Veuthey, J.-L., Anal. Bioanal. Chem. 2010, 397, 1069-1082. Liu, H., Finch, J. W., Lavallee, M. J., Collamati, R. A., Benevides, C. C., Gebler, J. C., J. Chromatogr. A 2007, 1147, 30-36. Vanrobaeys, F., Van Coster, R., Dhondt, G., Devreese, B., Van Beeumen, J., J. Proteome Res. 2005, 4, 2283-2293. Schrimpf, S. P., Weiss, M., Reiter, L., Ahrens, C. H., Jovanovic, M., Malmstrom, J., Brunner, E., Mohanty, S., Lercher, M. J., Hunziker, P. E., Aebersold, R., von Mering, C., Hengartner, M. O., PLoS Biol. 2009, 7, 616-627. 2010; 33 1997; 778 2002; 74 2009; 81 2006; 1107 2007; 1147 2012; 1228 2010; 1217 2006; 1104 2008; 624 1965; 37 1996; 743 2009; 14 2009; 1216 2009; 32 2010; 397 2005; 4 2011; 22 2009; 7 2012; 731 2012; 7 2006; 1131 1994; 5 2005; 77 2005; 1067 1999; 857 e_1_2_7_6_1 e_1_2_7_5_1 e_1_2_7_4_1 e_1_2_7_3_1 e_1_2_7_9_1 e_1_2_7_8_1 e_1_2_7_7_1 e_1_2_7_19_1 e_1_2_7_18_1 e_1_2_7_17_1 e_1_2_7_16_1 e_1_2_7_2_1 e_1_2_7_15_1 e_1_2_7_14_1 e_1_2_7_13_1 e_1_2_7_12_1 e_1_2_7_11_1 e_1_2_7_10_1 e_1_2_7_26_1 e_1_2_7_27_1 e_1_2_7_28_1 e_1_2_7_29_1 e_1_2_7_30_1 e_1_2_7_25_1 e_1_2_7_31_1 e_1_2_7_24_1 e_1_2_7_32_1 e_1_2_7_23_1 e_1_2_7_22_1 e_1_2_7_21_1 e_1_2_7_20_1 |
| References_xml | – volume: 857 start-page: 1 year: 1999 end-page: 20 publication-title: J. Chromatogr. A – volume: 778 start-page: 3 year: 1997 end-page: 21 publication-title: J. Chromatogr. A – volume: 1217 start-page: 6610 year: 2010 end-page: 6615 publication-title: J. Chromatogr. A – volume: 743 start-page: 99 year: 1996 end-page: 104 publication-title: J. Chromatogr. – volume: 32 start-page: 1129 year: 2009 end-page: 1136 publication-title: J. Sep. Sci. – volume: 14 start-page: 465 year: 2009 end-page: 471 publication-title: Drug Discovery Today – volume: 5 start-page: 867 year: 1994 end-page: 869 publication-title: J. Am. Soc. Mass Spectrom. – volume: 1217 start-page: 4779 year: 2010 end-page: 4783 publication-title: J. Chromatogr. A – volume: 1107 start-page: 139 year: 2006 end-page: 151 publication-title: J. Chromatogr. A – volume: 37 start-page: 60 year: 1965 end-page: 63 publication-title: Anal. Chem. – volume: 7 start-page: 616 year: 2009 end-page: 627 publication-title: PLoS Biol. – volume: 397 start-page: 1069 year: 2010 end-page: 1082 publication-title: Anal. Bioanal. Chem. – volume: 33 start-page: 2465 year: 2010 end-page: 2477 publication-title: J. Sep. Sci. – volume: 77 start-page: 2187 year: 2005 end-page: 2200 publication-title: Anal. Chem. – volume: 1104 start-page: 256 year: 2006 end-page: 262 publication-title: J. Chromatogr. A – volume: 74 start-page: 4235 year: 2002 end-page: 4249 publication-title: Anal. Chem. – volume: 4 start-page: 2283 year: 2005 end-page: 2293 publication-title: J. Proteome Res. – volume: 1216 start-page: 7368 year: 2009 end-page: 7374 publication-title: J. Chromatogr. A – volume: 1217 start-page: 2787 year: 2010 end-page: 2795 publication-title: J. Chromatogr. A – volume: 1147 start-page: 30 year: 2007 end-page: 36 publication-title: J. Chromatogr. A – volume: 1228 start-page: 270 year: 2012 end-page: 275 publication-title: J. Chromatogr. A – volume: 1131 start-page: 166 year: 2006 end-page: 175 publication-title: J. Chromatogr. A – volume: 1216 start-page: 3232 year: 2009 end-page: 3243 publication-title: J. Chromatogr. A – volume: 22 start-page: 3 year: 2011 end-page: 8 publication-title: Curr. Opin. Biotechnol. – volume: 33 start-page: 2629 year: 2010 end-page: 2635 publication-title: J. Sep. Sci. – volume: 81 start-page: 5342 year: 2009 end-page: 5353 publication-title: Anal. Chem. – volume: 7 start-page: 882 year: 2012 end-page: 890 publication-title: Nat. Protoc. – volume: 731 start-page: 1 year: 2012 end-page: 10 publication-title: Anal. Chim. Acta – volume: 1067 start-page: 73 year: 2005 end-page: 83 publication-title: J. Chromatogr. A – volume: 1228 start-page: 283 year: 2012 end-page: 291 publication-title: J. Chromatogr. A – volume: 624 start-page: 16 year: 2008 end-page: 36 publication-title: Anal. Chim. Acta – ident: e_1_2_7_21_1 doi: 10.1016/S0021-9673(97)00376-2 – ident: e_1_2_7_19_1 doi: 10.1016/j.chroma.2009.02.075 – ident: e_1_2_7_30_1 doi: 10.1016/j.chroma.2011.07.089 – ident: e_1_2_7_5_1 doi: 10.1016/j.chroma.2004.10.107 – ident: e_1_2_7_11_1 doi: 10.1038/nprot.2012.036 – ident: e_1_2_7_22_1 doi: 10.1016/j.chroma.2005.11.112 – ident: e_1_2_7_10_1 doi: 10.1371/journal.pbio.1000048 – ident: e_1_2_7_29_1 doi: 10.1016/j.chroma.2010.02.023 – ident: e_1_2_7_16_1 doi: 10.1002/jssc.200800639 – ident: e_1_2_7_12_1 doi: 10.1016/j.chroma.2010.05.015 – ident: e_1_2_7_2_1 doi: 10.1016/j.aca.2012.04.010 – ident: e_1_2_7_15_1 doi: 10.1021/ac0202280 – ident: e_1_2_7_18_1 doi: 10.1016/j.chroma.2011.12.088 – ident: e_1_2_7_28_1 doi: 10.1002/jssc.201000023 – ident: e_1_2_7_25_1 doi: 10.1002/jssc.201000339 – ident: e_1_2_7_17_1 doi: 10.1016/j.chroma.2010.03.037 – ident: e_1_2_7_20_1 doi: 10.1021/ac60220a012 – ident: e_1_2_7_13_1 doi: 10.1016/S0021-9673(99)00765-7 – ident: e_1_2_7_7_1 doi: 10.1016/0021-9673(96)00319-6 – ident: e_1_2_7_9_1 doi: 10.1016/j.copbio.2010.09.002 – ident: e_1_2_7_6_1 doi: 10.1016/j.chroma.2006.07.090 – ident: e_1_2_7_3_1 doi: 10.1016/j.aca.2008.06.017 – ident: e_1_2_7_23_1 doi: 10.1016/j.chroma.2009.02.032 – ident: e_1_2_7_26_1 doi: 10.1016/j.chroma.2005.12.050 – ident: e_1_2_7_32_1 doi: 10.1021/ac048455k – ident: e_1_2_7_4_1 doi: 10.1016/j.drudis.2009.02.007 – ident: e_1_2_7_31_1 doi: 10.1021/pr050205c – ident: e_1_2_7_14_1 doi: 10.1016/j.chroma.2007.02.016 – ident: e_1_2_7_8_1 doi: 10.1016/1044-0305(94)87010-1 – ident: e_1_2_7_24_1 doi: 10.1021/ac9001244 – ident: e_1_2_7_27_1 doi: 10.1007/s00216-009-3305-8 |
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| Snippet | We report on the optimization of nano‐LC gradient separations of proteomic samples that vary in complexity. The gradient performance limits were visualized by... We report on the optimization of nano-LC gradient separations of proteomic samples that vary in complexity. The gradient performance limits were visualized by... We report on the optimization of nano‐ LC gradient separations of proteomic samples that vary in complexity. The gradient performance limits were visualized by... |
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| SubjectTerms | Analytical, structural and metabolic biochemistry Biological and medical sciences Capillarity Chromatography Chromatography, High Pressure Liquid Column coupling Column packings Fundamental and applied biological sciences. Psychology General aspects, investigation methods Kinetic plot Nano flow rate Nanomaterials Nanostructure Nanotechnology - methods Optimization Packed capillary columns Particle Size Peptides Peptides - chemistry Peptides - isolation & purification Pressure Proteins Proteomics Separation Steepness Ultra-high pressures |
| Title | High-resolution peptide separations using nano-LC at ultra-high pressure |
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