Cell signalling regulates dynamics of Nanog distribution in embryonic stem cell populations

• Published in: Journal of the Royal Society interface Vol. 10; no. 78; p. 20120525
• Main Authors: Luo, Yang, Lim, Chea Lu, Nichols, Jennifer, Martinez-Arias, Alfonso, Wernisch, Lorenz
• Format: Journal Article
• Language: English
• Published: England The Royal Society 06.01.2013
• Subjects: Animals; Cell Culture Techniques; Cell Line; Embryonic Stem Cell; Glycogen Synthase Kinase 3 - genetics; Glycogen Synthase Kinase 3 - metabolism; Green Fluorescent Proteins - genetics; Green Fluorescent Proteins - metabolism; Homeodomain Proteins - genetics; Homeodomain Proteins - metabolism; MAP Kinase Signaling System - physiology; Mice; Models, Biological; Mouse Embryonic Stem Cells - cytology; Mouse Embryonic Stem Cells - metabolism; Nanog Homeobox Protein; Nanog Regulation; Protein Transport - physiology; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - metabolism; Stochastic System

A population of mouse embryonic stem (ES) cells is characterized by a distribution of Nanog, a gene whose expression is associated with the degree of pluripotency. Cells exhibiting high levels of Nanog maintain a state of pluripotency, while those with low levels are more likely to undergo different...