A multichannel system integrating molecularly imprinted conductive polymers for ultrasensitive voltammetric determination of four steroid hormones in urine

• Published in: Mikrochimica acta (1966) Vol. 186; no. 11; p. 695
• Main Authors: Lee, Mei-Hwa, Thomas, James L., Liu, Wei-Chiun, Zhang, Zheng-Xiang, Liu, Bin-Da, Yang, Chien-Hsin, Lin, Hung-Yin
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01.11.2019; Springer; Springer Nature B.V
• Subjects: Analytical Chemistry; Aniline; Aniline Compounds - chemistry; Book publishing; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Conducting polymers; Corticosteroids; Electrical engineering; Electrochemical Techniques - instrumentation; Electrochemical Techniques - methods; Electrodes; Equipment Design; Estradiol - urine; Estrogen; Hormones; Humans; Hydrocortisone - urine; Limit of Detection; Microengineering; Molecular Imprinting; Nanochemistry; Nanotechnology; Original Paper; Oxidation; Polymerization; Polymers; Polymers - chemical synthesis; Polymers - chemistry; Progesterone; Progesterone - urine; Sex hormones; Steroids; Sulfanilic Acids - chemistry; Testosterone; Testosterone - urine; Voltammetry; Electrochemical sensing; Multichannel potentiostat; Hormones; Urine analysis; Molecular imprinting
• ISSN: 0026-3672; 1436-5073; 1436-5073
• DOI: 10.1007/s00604-019-3797-7

This work reports on a modularized electrochemical method for the determination of the hormones cortisol, progesterone, testosterone and 17β-estradiol in urine. These hormones were employed as templates when generating molecular imprints from aniline and metanilic acid by electropolymerization on the surface of screen-printed electrodes. The electrically conductive imprint was characterized by SEM, AFM and cyclic voltammetry. A four-channel system was then established to enable simultaneous determination of the hormones by cyclic voltammetry. The detection limits for cortisol, progesterone, testosterone and 17β-estradiol are as low as 2, 2.5, 10 and 9 ag·mL −1 (for S/ N  = 3). Graphical abstract A four-channel system was established to enable simultaneous determination of 4 steroid hormones by cyclic voltammetry and by using moleculalry imprinted polymers.