Assessment of Extracellular Particles Directly in Diluted Plasma and Blood by Interferometric Light Microscopy. A Study of 613 Human and 163 Canine Samples

• Published in: Cells (Basel, Switzerland) Vol. 13; no. 24; p. 2054
• Main Authors: Korenjak, Boštjan, Tratenšek, Armando, Arko, Matevž, Romolo, Anna, Hočevar, Matej, Kisovec, Matic, Berry, Maxence, Bedina Zavec, Apolonija, Drobne, David, Vovk, Tomaž, Iglič, Aleš, Nemec Svete, Alenka, Erjavec, Vladimira, Kralj-Iglič, Veronika
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.12.2024; MDPI
• Subjects: Acetic acid; Airway management; Animals; Blood & organ donations; blood products; Cell interactions; Citric acid; Dilution; Disease; Dogs; Edetic acid; Electron microscopes; Ethylenediaminetetraacetic acid; exosomes; extracellular particles; extracellular vesicles; Female; Humans; Inflammatory bowel diseases; Light microscopy; membrane vesiculation; Microscope and microscopy; Microscopy; Microscopy, Interference - methods; Nanoparticles; Nanoparticles - chemistry; Plasma; Plasma - metabolism; platelets; Sodium citrate; Statistical analysis; Liberia; Massachusetts; Slovenia; United Kingdom > UK; membrane enclosed; blood products; extracellular particles; extracellular vesicles; exosomes; drug delivery; platelet-rich plasma; liquid biopsy; membrane vesiculation; platelets

Extracellular nanoparticles (EPs) are a subject of increasing interest for their biological role as mediators in cell–cell communication; however, their harvesting and assessment from bodily fluids are challenging, as processing can significantly affect samples. With the aim of minimizing processing...