The cell resealing technique for manipulating, visualizing, and elucidating molecular functions in living cells

• Published in: Biochimica et biophysica acta. General subjects Vol. 1864; no. 2; p. 129329
• Main Authors: Kunishige, Rina, Kano, Fumi, Murata, Masayuki
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.02.2020
• Subjects: animal disease models; cell membranes; Cell resealing technique; cytosol; diabetes; Disease model cell; drugs; image analysis; Intracellular delivery; Listeriolysin O; mice; permeability; phenotype; Quantitative image analysis; screening; streptolysin; Streptolysin O; Intracellular delivery; Cell resealing technique; Streptolysin O; Quantitative image analysis; ER; LLO; COP II; GFP; Listeriolysin O; Disease model cell; SLO; BICD; COP I; ATP

Cell-based assays are essential for analyzing molecular functions and spatiotemporal information. The cell resealing technique, in which pore-forming toxins are used to permeabilize cell membranes, enables the delivery of various membrane-impermeable molecules inside cells. We review the basics of t...