Tissue-engineered endothelial cell layers on surface-modified Ti for inhibiting in vitro platelet adhesion

• Published in: Science and technology of advanced materials Vol. 14; no. 3; pp. 35002 - 35009
• Main Authors: Wang, Xiupeng, He, Fupo, Li, Xia, Ito, Atsuo, Sogo, Yu, Maruyama, Osamu, Kosaka, Ryo, Ye, Jiandong
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis 01.06.2013; IOP Publishing; Taylor & Francis Ltd; Taylor & Francis Group
• Subjects: 10.01; 10.03; Adhesion; Apatite; ascorbate; Ascorbic acid; Calcium phosphates; endothelial cell; Endothelial cells; fibroblast growth factor-2; Growth factors; Magnesium; Metal plates; Nitric oxide; platelet adhesion; Surgical implants; Tissue engineering; Titanium; apatite; endothelial cell; ascorbate; 10.01; fibroblast growth factor-2; 10.03; platelet adhesion
• ISSN: 1468-6996; 1878-5514
• DOI: 10.1088/1468-6996/14/3/035002

A tissue-engineered endothelial layer was prepared by culturing endothelial cells on a fibroblast growth factor-2 (FGF-2)-l-ascorbic acid phosphate magnesium salt n-hydrate (AsMg)-apatite (Ap) coated titanium plate. The FGF-2-AsMg-Ap coated Ti plate was prepared by immersing a Ti plate in supersaturated calcium phosphate solutions supplemented with FGF-2 and AsMg. The FGF-2-AsMg-Ap layer on the Ti plate accelerated proliferation of human umbilical vein endothelial cells (HUVECs), and showed slightly higher, but not statistically significant, nitric oxide release from HUVECs than on as-prepared Ti. The endothelial layer maintained proper function of the endothelial cells and markedly inhibited in vitro platelet adhesion. The tissue-engineered endothelial layer formed on the FGF-2-AsMg-Ap layer is promising for ameliorating platelet activation and thrombus formation on cardiovascular implants.