Analysis of microRNA turnover in mammalian cells following Dicer1 ablation

• Published in: Nucleic acids research Vol. 39; no. 13; pp. 5692 - 5703
• Main Authors: Gantier, Michael P., McCoy, Claire E., Rusinova, Irina, Saulep, Damien, Wang, Die, Xu, Dakang, Irving, Aaron T., Behlke, Mark A., Hertzog, Paul J., Mackay, Fabienne, Williams, Bryan R. G.
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.07.2011
• Subjects: Animals; Cells, Cultured; DEAD-box RNA Helicases - genetics; DEAD-box RNA Helicases - metabolism; Gene Deletion; HEK293 Cells; Humans; Mice; MicroRNAs - metabolism; Models, Biological; Ribonuclease III - genetics; Ribonuclease III - metabolism; RNA; RNA Processing, Post-Transcriptional; RNA Stability

Although microRNAs (miRNAs) are key regulators of gene expression, little is known of their overall persistence in the cell following processing. Characterization of such persistence is key to the full appreciation of their regulatory roles. Accordingly, we measured miRNA decay rates in mouse embryo...