Analysis of microRNA turnover in mammalian cells following Dicer1 ablation
Although microRNAs (miRNAs) are key regulators of gene expression, little is known of their overall persistence in the cell following processing. Characterization of such persistence is key to the full appreciation of their regulatory roles. Accordingly, we measured miRNA decay rates in mouse embryo...
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| Published in | Nucleic acids research Vol. 39; no. 13; pp. 5692 - 5703 |
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| Main Authors | , , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
England
Oxford University Press
01.07.2011
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| Subjects | |
| Online Access | Get full text |
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| Abstract | Although microRNAs (miRNAs) are key regulators of gene expression, little is known of their overall persistence in the cell following processing. Characterization of such persistence is key to the full appreciation of their regulatory roles. Accordingly, we measured miRNA decay rates in mouse embryonic fibroblasts following loss of Dicer1 enzymatic activity. The results confirm the inherent stability of miRNAs, the intracellular levels of which were mostly affected by cell division. Using the decay rates of a panel of six miRNAs representative of the global trend of miRNA decay, we establish a mathematical model of miRNA turnover and determine an average miRNA half-life of 119 h (i.e. ∼5 days). In addition, we demonstrate that select miRNAs turnover more rapidly than others. This study constitutes, to our knowledge, the first in-depth characterization of miRNA decay in mammalian cells. Our findings indicate that miRNAs are up to 10× more stable than messenger RNA and support the existence of novel mechanism(s) controlling selective miRNA cellular concentration and function. |
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| AbstractList | Although microRNAs (miRNAs) are key regulators of gene expression, little is known of their overall persistence in the cell following processing. Characterization of such persistence is key to the full appreciation of their regulatory roles. Accordingly, we measured miRNA decay rates in mouse embryonic fibroblasts following loss of Dicer1 enzymatic activity. The results confirm the inherent stability of miRNAs, the intracellular levels of which were mostly affected by cell division. Using the decay rates of a panel of six miRNAs representative of the global trend of miRNA decay, we establish a mathematical model of miRNA turnover and determine an average miRNA half-life of 119 h (i.e. ∼5 days). In addition, we demonstrate that select miRNAs turnover more rapidly than others. This study constitutes, to our knowledge, the first in-depth characterization of miRNA decay in mammalian cells. Our findings indicate that miRNAs are up to 10× more stable than messenger RNA and support the existence of novel mechanism(s) controlling selective miRNA cellular concentration and function. Although microRNAs (miRNAs) are key regulators of gene expression, little is known of their overall persistence in the cell following processing. Characterization of such persistence is key to the full appreciation of their regulatory roles. Accordingly, we measured miRNA decay rates in mouse embryonic fibroblasts following loss of Dicer1 enzymatic activity. The results confirm the inherent stability of miRNAs, the intracellular levels of which were mostly affected by cell division. Using the decay rates of a panel of six miRNAs representative of the global trend of miRNA decay, we establish a mathematical model of miRNA turnover and determine an average miRNA half-life of 119 h (i.e. similar to 5 days). In addition, we demonstrate that select miRNAs turnover more rapidly than others. This study constitutes, to our knowledge, the first in-depth characterization of miRNA decay in mammalian cells. Our findings indicate that miRNAs are up to 10 more stable than messenger RNA and support the existence of novel mechanism(s) controlling selective miRNA cellular concentration and function. |
| Author | Gantier, Michael P. Saulep, Damien McCoy, Claire E. Rusinova, Irina Williams, Bryan R. G. Wang, Die Mackay, Fabienne Hertzog, Paul J. Xu, Dakang Irving, Aaron T. Behlke, Mark A. |
| AuthorAffiliation | 1 Centre for Cancer Research, 2 Centre for Innate Immunity and Infectious Diseases, Monash Institute of Medical Research, Monash University, Clayton, Victoria 3168, 3 Department of Immunology, Monash University, Alfred Medical Research and Education Precinct, Melbourne, Victoria 3004, Australia and 4 Integrated DNA Technologies Inc., Coralville, IA 52241, USA |
| AuthorAffiliation_xml | – name: 1 Centre for Cancer Research, 2 Centre for Innate Immunity and Infectious Diseases, Monash Institute of Medical Research, Monash University, Clayton, Victoria 3168, 3 Department of Immunology, Monash University, Alfred Medical Research and Education Precinct, Melbourne, Victoria 3004, Australia and 4 Integrated DNA Technologies Inc., Coralville, IA 52241, USA |
| Author_xml | – sequence: 1 givenname: Michael P. surname: Gantier fullname: Gantier, Michael P. organization: 1Centre for Cancer Research, 2Centre for Innate Immunity and Infectious Diseases, Monash Institute of Medical Research, Monash University, Clayton, Victoria 3168, 3Department of Immunology, Monash University, Alfred Medical Research and Education Precinct, Melbourne, Victoria 3004, Australia and 4Integrated DNA Technologies Inc., Coralville, IA 52241, USA – sequence: 2 givenname: Claire E. surname: McCoy fullname: McCoy, Claire E. organization: 1Centre for Cancer Research, 2Centre for Innate Immunity and Infectious Diseases, Monash Institute of Medical Research, Monash University, Clayton, Victoria 3168, 3Department of Immunology, Monash University, Alfred Medical Research and Education Precinct, Melbourne, Victoria 3004, Australia and 4Integrated DNA Technologies Inc., Coralville, IA 52241, USA – sequence: 3 givenname: Irina surname: Rusinova fullname: Rusinova, Irina organization: 1Centre for Cancer Research, 2Centre for Innate Immunity and Infectious Diseases, Monash Institute of Medical Research, Monash University, Clayton, Victoria 3168, 3Department of Immunology, Monash University, Alfred Medical Research and Education Precinct, Melbourne, Victoria 3004, Australia and 4Integrated DNA Technologies Inc., Coralville, IA 52241, USA – sequence: 4 givenname: Damien surname: Saulep fullname: Saulep, Damien organization: 1Centre for Cancer Research, 2Centre for Innate Immunity and Infectious Diseases, Monash Institute of Medical Research, Monash University, Clayton, Victoria 3168, 3Department of Immunology, Monash University, Alfred Medical Research and Education Precinct, Melbourne, Victoria 3004, Australia and 4Integrated DNA Technologies Inc., Coralville, IA 52241, USA – sequence: 5 givenname: Die surname: Wang fullname: Wang, Die organization: 1Centre for Cancer Research, 2Centre for Innate Immunity and Infectious Diseases, Monash Institute of Medical Research, Monash University, Clayton, Victoria 3168, 3Department of Immunology, Monash University, Alfred Medical Research and Education Precinct, Melbourne, Victoria 3004, Australia and 4Integrated DNA Technologies Inc., Coralville, IA 52241, USA – sequence: 6 givenname: Dakang surname: Xu fullname: Xu, Dakang organization: 1Centre for Cancer Research, 2Centre for Innate Immunity and Infectious Diseases, Monash Institute of Medical Research, Monash University, Clayton, Victoria 3168, 3Department of Immunology, Monash University, Alfred Medical Research and Education Precinct, Melbourne, Victoria 3004, Australia and 4Integrated DNA Technologies Inc., Coralville, IA 52241, USA – sequence: 7 givenname: Aaron T. surname: Irving fullname: Irving, Aaron T. organization: 1Centre for Cancer Research, 2Centre for Innate Immunity and Infectious Diseases, Monash Institute of Medical Research, Monash University, Clayton, Victoria 3168, 3Department of Immunology, Monash University, Alfred Medical Research and Education Precinct, Melbourne, Victoria 3004, Australia and 4Integrated DNA Technologies Inc., Coralville, IA 52241, USA – sequence: 8 givenname: Mark A. surname: Behlke fullname: Behlke, Mark A. organization: 1Centre for Cancer Research, 2Centre for Innate Immunity and Infectious Diseases, Monash Institute of Medical Research, Monash University, Clayton, Victoria 3168, 3Department of Immunology, Monash University, Alfred Medical Research and Education Precinct, Melbourne, Victoria 3004, Australia and 4Integrated DNA Technologies Inc., Coralville, IA 52241, USA – sequence: 9 givenname: Paul J. surname: Hertzog fullname: Hertzog, Paul J. organization: 1Centre for Cancer Research, 2Centre for Innate Immunity and Infectious Diseases, Monash Institute of Medical Research, Monash University, Clayton, Victoria 3168, 3Department of Immunology, Monash University, Alfred Medical Research and Education Precinct, Melbourne, Victoria 3004, Australia and 4Integrated DNA Technologies Inc., Coralville, IA 52241, USA – sequence: 10 givenname: Fabienne surname: Mackay fullname: Mackay, Fabienne organization: 1Centre for Cancer Research, 2Centre for Innate Immunity and Infectious Diseases, Monash Institute of Medical Research, Monash University, Clayton, Victoria 3168, 3Department of Immunology, Monash University, Alfred Medical Research and Education Precinct, Melbourne, Victoria 3004, Australia and 4Integrated DNA Technologies Inc., Coralville, IA 52241, USA – sequence: 11 givenname: Bryan R. G. surname: Williams fullname: Williams, Bryan R. G. email: bryan.williams@monash.edu organization: 1Centre for Cancer Research, 2Centre for Innate Immunity and Infectious Diseases, Monash Institute of Medical Research, Monash University, Clayton, Victoria 3168, 3Department of Immunology, Monash University, Alfred Medical Research and Education Precinct, Melbourne, Victoria 3004, Australia and 4Integrated DNA Technologies Inc., Coralville, IA 52241, USA |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/21447562$$D View this record in MEDLINE/PubMed |
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| Copyright | The Author(s) 2011. Published by Oxford University Press. 2011 |
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| Title | Analysis of microRNA turnover in mammalian cells following Dicer1 ablation |
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