Infectious Spleen and Kidney Necrosis Virus (a Fish Iridovirus) Enters Mandarin Fish Fry Cells via Caveola-Dependent Endocytosis

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Published inJournal of Virology Vol. 86; no. 5; pp. 2621 - 2631
Main Authors Guo, Chang-Jun, Wu, Yan-Yan, Yang, Li-Shi, Yang, Xiao-Bo, He, Jian, Mi, Shu, Jia, Kun-Tong, Weng, Shao-Ping, Yu, Xiao-Qiang, He, Jian-Guo
Format Journal Article
LanguageEnglish
Published Washington, DC American Society for Microbiology 01.03.2012
Subjects
Animals
beta-Cyclodextrins - metabolism
Biological and medical sciences
Caveolin 1 - metabolism
Cell Line
China
DNA Virus Infections - metabolism
DNA Virus Infections - physiopathology
DNA Virus Infections - veterinary
DNA Virus Infections - virology
Endocytosis
Fish Diseases - metabolism
Fish Diseases - physiopathology
Fish Diseases - virology
Fishes
Fundamental and applied biological sciences. Psychology
Iridovirus - genetics
Iridovirus - physiology
Microbiology
Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains
Virology
Virus-Cell Interactions
China
Virus
Mammalian type C retrovirus
Vertebrata
Endocytosis
Iridoviridae
Spleen necrosis virus
Internalization
Pisces
Retroviridae
In vitro
Iridovirus
Kidney
Online AccessGet full text

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Abstract Article Usage Stats Services JVI Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue Spotlights in the Current Issue JVI About JVI Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy JVI RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0022-538X Online ISSN: 1098-5514 Copyright © 2014 by the American Society for Microbiology.   For an alternate route to JVI .asm.org, visit: JVI       
AbstractList Infectious spleen and kidney necrosis virus (ISKNV) is the type species of the genus Megalocytivirus from the family Iridoviridae . Megalocytiviruses have been implicated in more than 50 fish species infections and currently threaten the aquaculture industry, causing great economic losses in China, Japan, and Southeast Asia. However, the cellular entry mechanisms of megalocytiviruses remain largely uncharacterized. In this study, the main internalization mechanism of ISKNV was investigated by using mandarin fish fry (MFF-1) cells. The progression of ISKNV infection is slow, and infection is not inhibited when the cells are treated with ammonium chloride (NH 4 Cl), chloroquine, sucrose, and chlorpromazine, which are inhibitors of clathrin-dependent endocytosis. The depletion of cellular cholesterol by methyl-β-cyclodextrin results in the significant inhibition of ISKNV infection; however, the infection is resumed with cholesterol replenishment. Inhibitors of caveolin-1-involved signaling events, including phorbol 12-myristate 13-acetate (PMA), genistein, and wortmannin, impair ISKNV entry into MFF-1 cells. Moreover, ISKNV entry is dependent on dynamin and the microtubule cytoskeleton. Cofraction analysis of ISKNV and caveolin-1 showed that ISKNV colocates with caveolin-1 during virus infection. These results indicate that ISKNV entry into MFF-1 cells proceeds via classical caveola-mediated endocytosis and is dependent on the microtubules that serve as tracks along which motile cavicles may move via a caveola-caveosome-endoplasmic reticulum (ER) pathway. As a fish iridovirus, ISKNV entry into MFF-1 cells is different from the clathrin-mediated endocytosis of frog virus 3 entry into mammalian cells (BHK-21) at 28°C, which has been recognized as a model for iridoviruses. Thus, our work may help further the understanding of the initial steps of iridovirus infection.
Infectious spleen and kidney necrosis virus (ISKNV) is the type species of the genus Megalocytivirus from the family Iridoviridae. Megalocytiviruses have been implicated in more than 50 fish species infections and currently threaten the aquaculture industry, causing great economic losses in China, Japan, and Southeast Asia. However, the cellular entry mechanisms of megalocytiviruses remain largely uncharacterized. In this study, the main internalization mechanism of ISKNV was investigated by using mandarin fish fry (MFF-1) cells. The progression of ISKNV infection is slow, and infection is not inhibited when the cells are treated with ammonium chloride (NH(4)Cl), chloroquine, sucrose, and chlorpromazine, which are inhibitors of clathrin-dependent endocytosis. The depletion of cellular cholesterol by methyl-β-cyclodextrin results in the significant inhibition of ISKNV infection; however, the infection is resumed with cholesterol replenishment. Inhibitors of caveolin-1-involved signaling events, including phorbol 12-myristate 13-acetate (PMA), genistein, and wortmannin, impair ISKNV entry into MFF-1 cells. Moreover, ISKNV entry is dependent on dynamin and the microtubule cytoskeleton. Cofraction analysis of ISKNV and caveolin-1 showed that ISKNV colocates with caveolin-1 during virus infection. These results indicate that ISKNV entry into MFF-1 cells proceeds via classical caveola-mediated endocytosis and is dependent on the microtubules that serve as tracks along which motile cavicles may move via a caveola-caveosome-endoplasmic reticulum (ER) pathway. As a fish iridovirus, ISKNV entry into MFF-1 cells is different from the clathrin-mediated endocytosis of frog virus 3 entry into mammalian cells (BHK-21) at 28°C, which has been recognized as a model for iridoviruses. Thus, our work may help further the understanding of the initial steps of iridovirus infection.Infectious spleen and kidney necrosis virus (ISKNV) is the type species of the genus Megalocytivirus from the family Iridoviridae. Megalocytiviruses have been implicated in more than 50 fish species infections and currently threaten the aquaculture industry, causing great economic losses in China, Japan, and Southeast Asia. However, the cellular entry mechanisms of megalocytiviruses remain largely uncharacterized. In this study, the main internalization mechanism of ISKNV was investigated by using mandarin fish fry (MFF-1) cells. The progression of ISKNV infection is slow, and infection is not inhibited when the cells are treated with ammonium chloride (NH(4)Cl), chloroquine, sucrose, and chlorpromazine, which are inhibitors of clathrin-dependent endocytosis. The depletion of cellular cholesterol by methyl-β-cyclodextrin results in the significant inhibition of ISKNV infection; however, the infection is resumed with cholesterol replenishment. Inhibitors of caveolin-1-involved signaling events, including phorbol 12-myristate 13-acetate (PMA), genistein, and wortmannin, impair ISKNV entry into MFF-1 cells. Moreover, ISKNV entry is dependent on dynamin and the microtubule cytoskeleton. Cofraction analysis of ISKNV and caveolin-1 showed that ISKNV colocates with caveolin-1 during virus infection. These results indicate that ISKNV entry into MFF-1 cells proceeds via classical caveola-mediated endocytosis and is dependent on the microtubules that serve as tracks along which motile cavicles may move via a caveola-caveosome-endoplasmic reticulum (ER) pathway. As a fish iridovirus, ISKNV entry into MFF-1 cells is different from the clathrin-mediated endocytosis of frog virus 3 entry into mammalian cells (BHK-21) at 28°C, which has been recognized as a model for iridoviruses. Thus, our work may help further the understanding of the initial steps of iridovirus infection.
Article Usage Stats Services JVI Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue Spotlights in the Current Issue JVI About JVI Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy JVI RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0022-538X Online ISSN: 1098-5514 Copyright © 2014 by the American Society for Microbiology.   For an alternate route to JVI .asm.org, visit: JVI       
Infectious spleen and kidney necrosis virus (ISKNV) is the type species of the genus Megalocytivirus from the family Iridoviridae. Megalocytiviruses have been implicated in more than 50 fish species infections and currently threaten the aquaculture industry, causing great economic losses in China, Japan, and Southeast Asia. However, the cellular entry mechanisms of megalocytiviruses remain largely uncharacterized. In this study, the main internalization mechanism of ISKNV was investigated by using mandarin fish fry (MFF-1) cells. The progression of ISKNV infection is slow, and infection is not inhibited when the cells are treated with ammonium chloride (NH(4)Cl), chloroquine, sucrose, and chlorpromazine, which are inhibitors of clathrin-dependent endocytosis. The depletion of cellular cholesterol by methyl-β-cyclodextrin results in the significant inhibition of ISKNV infection; however, the infection is resumed with cholesterol replenishment. Inhibitors of caveolin-1-involved signaling events, including phorbol 12-myristate 13-acetate (PMA), genistein, and wortmannin, impair ISKNV entry into MFF-1 cells. Moreover, ISKNV entry is dependent on dynamin and the microtubule cytoskeleton. Cofraction analysis of ISKNV and caveolin-1 showed that ISKNV colocates with caveolin-1 during virus infection. These results indicate that ISKNV entry into MFF-1 cells proceeds via classical caveola-mediated endocytosis and is dependent on the microtubules that serve as tracks along which motile cavicles may move via a caveola-caveosome-endoplasmic reticulum (ER) pathway. As a fish iridovirus, ISKNV entry into MFF-1 cells is different from the clathrin-mediated endocytosis of frog virus 3 entry into mammalian cells (BHK-21) at 28°C, which has been recognized as a model for iridoviruses. Thus, our work may help further the understanding of the initial steps of iridovirus infection.
Author Jian He
Li-Shi Yang
Kun-Tong Jia
Jian-Guo He
Xiao-Bo Yang
Shao-Ping Weng
Yan-Yan Wu
Xiao-Qiang Yu
Chang-Jun Guo
Shu Mi
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  organization: MOE Key Laboratory of Aquatic Product Safety, School of Marine Science, Sun Yat-sen University, Guangzhou, People's Republic of China, State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, People's Republic of China
– sequence: 2
  givenname: Yan-Yan
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  fullname: Wu, Yan-Yan
  organization: State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, People's Republic of China
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  givenname: Li-Shi
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  organization: State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, People's Republic of China
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  givenname: Xiao-Qiang
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Cites_doi 10.1128/JVI.76.4.1856-1865.2002
10.1242/jcs.00840
10.1080/09687860500460041
10.1128/JVI.78.5.2310-2318.2004
10.1242/jcs.00327
10.1371/journal.pbio.0030233
10.1242/jcs.112.22.3899
10.1073/pnas.0803711105
10.1016/j.cardiores.2007.03.023
10.1128/JVI.74.5.2288-2292.2000
10.1128/JVI.78.6.3089-3098.2004
10.1006/viro.2001.1303
10.1128/JVI.79.16.10776-10787.2005
10.1152/physrev.00046.2003
10.1242/jcs.111.6.825
10.1128/JVI.78.21.11583-11590.2004
10.1074/jbc.M111240200
10.3390/v3101959
10.1099/vir.0.82808-0
10.1128/JVI.78.19.10543-10555.2004
10.1016/j.cellsig.2009.07.010
10.1016/j.bbamcr.2005.06.009
10.1016/j.chom.2007.10.007
10.1093/emboj/17.16.4585
10.1128/JVI.79.13.8519-8534.2005
10.1007/978-1-4684-3563-4_6
10.1083/jcb.141.1.101
10.1111/j.1348-0421.2004.tb03614.x
10.1002/j.1460-2075.1986.tb04616.x
10.1073/pnas.92.19.8655
10.1016/j.devcel.2006.04.002
10.1038/35074539
10.1128/JVI.00988-07
10.1128/JVI.00024-06
10.1139/W08-107
10.1016/j.chom.2010.05.008
10.1099/0022-1317-66-2-283
10.1034/j.1600-0854.2002.30501.x
10.1128/JVI.01086-06
10.1128/JVI.01557-09
10.1128/JVI.01500-10
10.1016/j.molimm.2011.01.001
10.1083/jcb.200302028
10.1091/mbc.7.11.1825
10.1128/JVI.78.16.8701-8708.2004
10.1083/jcb.200407113
10.1016/j.cell.2005.12.038
10.1016/j.mcp.2005.12.002
10.1046/j.1365-2761.2000.00213.x
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Issue 5
Keywords Virus
Mammalian type C retrovirus
Vertebrata
Endocytosis
Iridoviridae
Spleen necrosis virus
Internalization
Pisces
Retroviridae
In vitro
Iridovirus
Kidney
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C.-J. Guo and Y.-Y. Wu contributed equally to this work.
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References e_1_3_2_26_2
e_1_3_2_49_2
e_1_3_2_28_2
Dong C (e_1_3_2_15_2) 2011; 85
e_1_3_2_41_2
e_1_3_2_20_2
e_1_3_2_43_2
e_1_3_2_22_2
e_1_3_2_45_2
e_1_3_2_24_2
e_1_3_2_47_2
e_1_3_2_16_2
e_1_3_2_37_2
e_1_3_2_7_2
e_1_3_2_18_2
e_1_3_2_39_2
Chinchar VG (e_1_3_2_9_2) 2009; 328
e_1_3_2_10_2
e_1_3_2_31_2
e_1_3_2_52_2
e_1_3_2_5_2
e_1_3_2_12_2
e_1_3_2_33_2
e_1_3_2_3_2
e_1_3_2_14_2
e_1_3_2_35_2
e_1_3_2_50_2
e_1_3_2_27_2
e_1_3_2_48_2
e_1_3_2_29_2
e_1_3_2_40_2
e_1_3_2_21_2
e_1_3_2_42_2
e_1_3_2_23_2
e_1_3_2_44_2
e_1_3_2_25_2
e_1_3_2_46_2
e_1_3_2_38_2
e_1_3_2_8_2
e_1_3_2_17_2
e_1_3_2_6_2
e_1_3_2_19_2
e_1_3_2_30_2
e_1_3_2_32_2
e_1_3_2_51_2
e_1_3_2_11_2
e_1_3_2_34_2
e_1_3_2_4_2
e_1_3_2_13_2
e_1_3_2_36_2
e_1_3_2_2_2
8930903 - Mol Biol Cell. 1996 Nov;7(11):1825-34
11799180 - J Virol. 2002 Feb;76(4):1856-65
15383654 - Physiol Rev. 2004 Oct;84(4):1341-79
16740485 - Dev Cell. 2006 Jun;10(6):839-50
19643177 - Cell Signal. 2009 Dec;21(12):1748-57
16530046 - Cell. 2006 Mar 10;124(5):997-1009
17626097 - J Virol. 2007 Sep;81(18):9922-31
14600257 - J Cell Sci. 2003 Dec 1;116(Pt 23):4707-14
18005749 - Cell Host Microbe. 2007 Nov 15;2(5):288-90
11331875 - Nat Cell Biol. 2001 May;3(5):473-83
11724808 - J Biol Chem. 2002 Feb 1;277(5):3371-9
17485539 - J Gen Virol. 2007 Jun;88(Pt 6):1776-84
20542247 - Cell Host Microbe. 2010 Jun 25;7(6):427-39
10666259 - J Virol. 2000 Mar;74(5):2288-92
15954801 - PLoS Biol. 2005 Jul;3(7):e233
17466957 - Cardiovasc Res. 2007 Jul 15;75(2):247-60
12584249 - J Cell Sci. 2003 Mar 15;116(Pt 6):1059-71
16051869 - J Virol. 2005 Aug;79(16):10776-87
15668298 - J Cell Biol. 2005 Jan 31;168(3):477-88
14963127 - J Virol. 2004 Mar;78(5):2310-8
15280478 - J Virol. 2004 Aug;78(16):8701-8
15956594 - J Virol. 2005 Jul;79(13):8519-34
11967125 - Traffic. 2002 May;3(5):311-20
3918142 - J Gen Virol. 1985 Feb;66 ( Pt 2):283-93
15557740 - Microbiol Immunol. 2004;48(11):823-9
21209107 - J Virol. 2011 Mar;85(6):2869-77
9531551 - J Cell Biol. 1998 Apr 6;141(1):101-14
11886265 - Virology. 2002 Mar 1;294(1):60-9
19939916 - J Virol. 2010 Feb;84(4):2100-9
15367621 - J Virol. 2004 Oct;78(19):10543-55
21543502 - J Virol. 2011 Jul;85(13):6416-26
3816755 - EMBO J. 1986 Dec 1;5(12):3103-9
21296425 - Mol Immunol. 2011 Apr;48(8):992-1000
10547351 - J Cell Sci. 1999 Nov;112 ( Pt 22):3899-909
16973546 - J Virol. 2006 Oct;80(19):9402-13
19216437 - Curr Top Microbiol Immunol. 2009;328:123-70
12771123 - J Cell Biol. 2003 May 26;161(4):673-7
15479799 - J Virol. 2004 Nov;78(21):11583-90
16809302 - J Virol. 2006 Jul;80(14):6964-72
7567992 - Proc Natl Acad Sci U S A. 1995 Sep 12;92(19):8655-9
16611585 - Mol Membr Biol. 2006 Jan-Feb;23(1):101-10
16697343 - Mol Cell Probes. 2006 Jun-Aug;20(3-4):212-22
19295646 - Can J Microbiol. 2009 Feb;55(2):139-45
22069524 - Viruses. 2011 Oct;3(10):1959-85
14990728 - J Virol. 2004 Mar;78(6):3089-98
9707418 - EMBO J. 1998 Aug 17;17(16):4585-93
18689690 - Proc Natl Acad Sci U S A. 2008 Aug 19;105(33):11790-5
9472010 - J Cell Sci. 1998 Mar;111 ( Pt 6):825-32
16126288 - Biochim Biophys Acta. 2005 Dec 30;1746(3):295-304
References_xml – ident: e_1_3_2_34_2
  doi: 10.1128/JVI.76.4.1856-1865.2002
– ident: e_1_3_2_37_2
  doi: 10.1242/jcs.00840
– volume: 85
  start-page: 2869
  year: 2011
  ident: e_1_3_2_15_2
  publication-title: Global landscape of structural proteins of infectious spleen and kidney necrosis virus.
– ident: e_1_3_2_8_2
  doi: 10.1080/09687860500460041
– ident: e_1_3_2_47_2
  doi: 10.1128/JVI.78.5.2310-2318.2004
– ident: e_1_3_2_32_2
  doi: 10.1242/jcs.00327
– ident: e_1_3_2_51_2
  doi: 10.1371/journal.pbio.0030233
– ident: e_1_3_2_48_2
  doi: 10.1242/jcs.112.22.3899
– ident: e_1_3_2_7_2
  doi: 10.1073/pnas.0803711105
– ident: e_1_3_2_16_2
  doi: 10.1016/j.cardiores.2007.03.023
– ident: e_1_3_2_45_2
  doi: 10.1128/JVI.74.5.2288-2292.2000
– ident: e_1_3_2_26_2
  doi: 10.1128/JVI.78.6.3089-3098.2004
– ident: e_1_3_2_27_2
  doi: 10.1006/viro.2001.1303
– ident: e_1_3_2_3_2
  doi: 10.1128/JVI.79.16.10776-10787.2005
– ident: e_1_3_2_12_2
  doi: 10.1152/physrev.00046.2003
– ident: e_1_3_2_50_2
  doi: 10.1242/jcs.111.6.825
– ident: e_1_3_2_17_2
  doi: 10.1128/JVI.78.21.11583-11590.2004
– ident: e_1_3_2_31_2
  doi: 10.1074/jbc.M111240200
– ident: e_1_3_2_10_2
  doi: 10.3390/v3101959
– ident: e_1_3_2_35_2
  doi: 10.1099/vir.0.82808-0
– ident: e_1_3_2_11_2
  doi: 10.1128/JVI.78.19.10543-10555.2004
– ident: e_1_3_2_19_2
  doi: 10.1016/j.cellsig.2009.07.010
– ident: e_1_3_2_43_2
  doi: 10.1016/j.bbamcr.2005.06.009
– ident: e_1_3_2_40_2
  doi: 10.1016/j.chom.2007.10.007
– ident: e_1_3_2_14_2
  doi: 10.1093/emboj/17.16.4585
– ident: e_1_3_2_4_2
  doi: 10.1128/JVI.79.13.8519-8534.2005
– ident: e_1_3_2_21_2
  doi: 10.1007/978-1-4684-3563-4_6
– ident: e_1_3_2_39_2
  doi: 10.1083/jcb.141.1.101
– ident: e_1_3_2_28_2
  doi: 10.1111/j.1348-0421.2004.tb03614.x
– ident: e_1_3_2_29_2
  doi: 10.1002/j.1460-2075.1986.tb04616.x
– ident: e_1_3_2_18_2
  doi: 10.1073/pnas.92.19.8655
– ident: e_1_3_2_33_2
  doi: 10.1016/j.devcel.2006.04.002
– ident: e_1_3_2_42_2
  doi: 10.1038/35074539
– ident: e_1_3_2_49_2
  doi: 10.1128/JVI.00988-07
– volume: 328
  start-page: 123
  year: 2009
  ident: e_1_3_2_9_2
  article-title: Family Iridoviridae: poor viral relations no longer
  publication-title: Curr. Top. Microbiol. Immunol.
– ident: e_1_3_2_5_2
  doi: 10.1128/JVI.00024-06
– ident: e_1_3_2_44_2
  doi: 10.1139/W08-107
– ident: e_1_3_2_52_2
  doi: 10.1016/j.chom.2010.05.008
– ident: e_1_3_2_6_2
  doi: 10.1099/0022-1317-66-2-283
– ident: e_1_3_2_41_2
  doi: 10.1034/j.1600-0854.2002.30501.x
– ident: e_1_3_2_46_2
  doi: 10.1128/JVI.01086-06
– ident: e_1_3_2_25_2
  doi: 10.1128/JVI.01557-09
– ident: e_1_3_2_22_2
  doi: 10.1128/JVI.01500-10
– ident: e_1_3_2_23_2
  doi: 10.1016/j.molimm.2011.01.001
– ident: e_1_3_2_36_2
  doi: 10.1083/jcb.200302028
– ident: e_1_3_2_2_2
  doi: 10.1091/mbc.7.11.1825
– ident: e_1_3_2_38_2
  doi: 10.1128/JVI.78.16.8701-8708.2004
– ident: e_1_3_2_13_2
  doi: 10.1083/jcb.200407113
– ident: e_1_3_2_30_2
  doi: 10.1016/j.cell.2005.12.038
– ident: e_1_3_2_20_2
  doi: 10.1016/j.mcp.2005.12.002
– ident: e_1_3_2_24_2
  doi: 10.1046/j.1365-2761.2000.00213.x
– reference: 15668298 - J Cell Biol. 2005 Jan 31;168(3):477-88
– reference: 16740485 - Dev Cell. 2006 Jun;10(6):839-50
– reference: 11799180 - J Virol. 2002 Feb;76(4):1856-65
– reference: 17485539 - J Gen Virol. 2007 Jun;88(Pt 6):1776-84
– reference: 3918142 - J Gen Virol. 1985 Feb;66 ( Pt 2):283-93
– reference: 15479799 - J Virol. 2004 Nov;78(21):11583-90
– reference: 16611585 - Mol Membr Biol. 2006 Jan-Feb;23(1):101-10
– reference: 15383654 - Physiol Rev. 2004 Oct;84(4):1341-79
– reference: 19295646 - Can J Microbiol. 2009 Feb;55(2):139-45
– reference: 3816755 - EMBO J. 1986 Dec 1;5(12):3103-9
– reference: 10666259 - J Virol. 2000 Mar;74(5):2288-92
– reference: 12771123 - J Cell Biol. 2003 May 26;161(4):673-7
– reference: 21209107 - J Virol. 2011 Mar;85(6):2869-77
– reference: 7567992 - Proc Natl Acad Sci U S A. 1995 Sep 12;92(19):8655-9
– reference: 15557740 - Microbiol Immunol. 2004;48(11):823-9
– reference: 20542247 - Cell Host Microbe. 2010 Jun 25;7(6):427-39
– reference: 17626097 - J Virol. 2007 Sep;81(18):9922-31
– reference: 11331875 - Nat Cell Biol. 2001 May;3(5):473-83
– reference: 9472010 - J Cell Sci. 1998 Mar;111 ( Pt 6):825-32
– reference: 19939916 - J Virol. 2010 Feb;84(4):2100-9
– reference: 16051869 - J Virol. 2005 Aug;79(16):10776-87
– reference: 10547351 - J Cell Sci. 1999 Nov;112 ( Pt 22):3899-909
– reference: 16126288 - Biochim Biophys Acta. 2005 Dec 30;1746(3):295-304
– reference: 12584249 - J Cell Sci. 2003 Mar 15;116(Pt 6):1059-71
– reference: 15956594 - J Virol. 2005 Jul;79(13):8519-34
– reference: 19216437 - Curr Top Microbiol Immunol. 2009;328:123-70
– reference: 9531551 - J Cell Biol. 1998 Apr 6;141(1):101-14
– reference: 14963127 - J Virol. 2004 Mar;78(5):2310-8
– reference: 16973546 - J Virol. 2006 Oct;80(19):9402-13
– reference: 11886265 - Virology. 2002 Mar 1;294(1):60-9
– reference: 11967125 - Traffic. 2002 May;3(5):311-20
– reference: 17466957 - Cardiovasc Res. 2007 Jul 15;75(2):247-60
– reference: 18005749 - Cell Host Microbe. 2007 Nov 15;2(5):288-90
– reference: 9707418 - EMBO J. 1998 Aug 17;17(16):4585-93
– reference: 16697343 - Mol Cell Probes. 2006 Jun-Aug;20(3-4):212-22
– reference: 15280478 - J Virol. 2004 Aug;78(16):8701-8
– reference: 16809302 - J Virol. 2006 Jul;80(14):6964-72
– reference: 22069524 - Viruses. 2011 Oct;3(10):1959-85
– reference: 21543502 - J Virol. 2011 Jul;85(13):6416-26
– reference: 8930903 - Mol Biol Cell. 1996 Nov;7(11):1825-34
– reference: 14990728 - J Virol. 2004 Mar;78(6):3089-98
– reference: 11724808 - J Biol Chem. 2002 Feb 1;277(5):3371-9
– reference: 16530046 - Cell. 2006 Mar 10;124(5):997-1009
– reference: 18689690 - Proc Natl Acad Sci U S A. 2008 Aug 19;105(33):11790-5
– reference: 21296425 - Mol Immunol. 2011 Apr;48(8):992-1000
– reference: 14600257 - J Cell Sci. 2003 Dec 1;116(Pt 23):4707-14
– reference: 19643177 - Cell Signal. 2009 Dec;21(12):1748-57
– reference: 15954801 - PLoS Biol. 2005 Jul;3(7):e233
– reference: 15367621 - J Virol. 2004 Oct;78(19):10543-55
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Infectious spleen and kidney necrosis virus (ISKNV) is the type species of the genus Megalocytivirus from the family Iridoviridae . Megalocytiviruses have been...
Infectious spleen and kidney necrosis virus (ISKNV) is the type species of the genus Megalocytivirus from the family Iridoviridae. Megalocytiviruses have been...
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StartPage 2621
SubjectTerms Animals
beta-Cyclodextrins - metabolism
Biological and medical sciences
Caveolin 1 - metabolism
Cell Line
China
DNA Virus Infections - metabolism
DNA Virus Infections - physiopathology
DNA Virus Infections - veterinary
DNA Virus Infections - virology
Endocytosis
Fish Diseases - metabolism
Fish Diseases - physiopathology
Fish Diseases - virology
Fishes
Fundamental and applied biological sciences. Psychology
Iridovirus - genetics
Iridovirus - physiology
Microbiology
Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains
Virology
Virus-Cell Interactions
Title Infectious Spleen and Kidney Necrosis Virus (a Fish Iridovirus) Enters Mandarin Fish Fry Cells via Caveola-Dependent Endocytosis
URI http://jvi.asm.org/content/86/5/2621.abstract
https://www.ncbi.nlm.nih.gov/pubmed/22171272
https://www.proquest.com/docview/921423204
https://pubmed.ncbi.nlm.nih.gov/PMC3302275
Volume 86
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