Use of Microfluidic Capillary Electrophoresis for the Determination of Multi-Component Protein Adsorption Isotherms: Application to High-Throughput Analysis for Hydrophobic Interaction Chromatography

• Published in: Pharmaceutics Vol. 13; no. 12; p. 2135
• Main Authors: Lietta, Elena, Pieri, Alessandro, Innocenti, Elisa, Pisano, Roberto, Vanni, Marco, Barresi, Antonello A
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 11.12.2021; MDPI
• Subjects: Accuracy; Adsorption; Capillary electrophoresis; Chromatography; Efficiency; high-throughput chromatography; hydrophobic interaction chromatography; Investigations; Methods; Molecular weight; multi-component adsorption isotherms; Potash; Potassium; Proteins; Reproducibility; New York; Italy; Sweden; St Louis Missouri; United States > US; multi-component adsorption isotherms; high-throughput chromatography; capillary electrophoresis; hydrophobic interaction chromatography

Chromatography is a widely used separation process for purification of biopharmaceuticals that is able to obtain high purities and concentrations. The phenomena that occur during separation, mass transfer and adsorption are quite complex. To better understand these phenomena and their mechanisms, mu...