Efficient Multiplication of Human Metapneumovirus in Vero Cells Expressing the Transmembrane Serine Protease TMPRSS2
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| Published in | Journal of Virology Vol. 82; no. 17; pp. 8942 - 8946 |
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| Main Authors | , , , , , , , , |
| Format | Journal Article |
| Language | English |
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Washington, DC
American Society for Microbiology
01.09.2008
American Society for Microbiology (ASM) |
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| AbstractList | Human metapneumovirus (HMPV) is a major causative agent of severe bronchiolitis and pneumonia. Its fusion (F) protein must be cleaved by host proteases to cause membrane fusion, a critical step for virus infection. By generating Vero cells constitutively expressing the transmembrane serine protease TMPRSS2 and green fluorescent protein-expressing recombinant HMPV, we show that TMPRSS2, which is expressed in the human lung epithelium, cleaves the HMPV F protein efficiently and supports HMPV multiplication. The results indicate that TMPRSS2 is a possible candidate protease involved in the development of lower respiratory tract illness in HMPV-infected patients. Human metapneumovirus (HMPV) is a major causative agent of severe bronchiolitis and pneumonia. Its fusion (F) protein must be cleaved by host proteases to cause membrane fusion, a critical step for virus infection. By generating Vero cells constitutively expressing the transmembrane serine protease TMPRSS2 and green fluorescent protein-expressing recombinant HMPV, we show that TMPRSS2, which is expressed in the human lung epithelium, cleaves the HMPV F protein efficiently and supports HMPV multiplication. The results indicate that TMPRSS2 is a possible candidate protease involved in the development of lower respiratory tract illness in HMPV-infected patients.Human metapneumovirus (HMPV) is a major causative agent of severe bronchiolitis and pneumonia. Its fusion (F) protein must be cleaved by host proteases to cause membrane fusion, a critical step for virus infection. By generating Vero cells constitutively expressing the transmembrane serine protease TMPRSS2 and green fluorescent protein-expressing recombinant HMPV, we show that TMPRSS2, which is expressed in the human lung epithelium, cleaves the HMPV F protein efficiently and supports HMPV multiplication. The results indicate that TMPRSS2 is a possible candidate protease involved in the development of lower respiratory tract illness in HMPV-infected patients. Article Usage Stats Services JVI Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue Spotlights in the Current Issue JVI About JVI Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy JVI RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0022-538X Online ISSN: 1098-5514 Copyright © 2014 by the American Society for Microbiology. For an alternate route to JVI .asm.org, visit: JVI |
| Author | Makoto Takeda Yuta Shirogane Hiroki Takeuchi Nobuhisa Ishiguro Yusuke Yanagi Maino Tahara Hideaki Kikuta Yuichiro Nakatsu Masaharu Iwasaki |
| AuthorAffiliation | Department of Virology, Faculty of Medicine, Kyushu University, Fukuoka 812-8582, 1 Department of Pediatrics, Hokkaido University Graduate School of Medicine, Sapporo 060-8638, 2 Pediatric Clinic, Touei Hospital, Kita-41, Higashi-6, Higashi-ku, Sapporo 007-0841, Japan 3 |
| AuthorAffiliation_xml | – name: Department of Virology, Faculty of Medicine, Kyushu University, Fukuoka 812-8582, 1 Department of Pediatrics, Hokkaido University Graduate School of Medicine, Sapporo 060-8638, 2 Pediatric Clinic, Touei Hospital, Kita-41, Higashi-6, Higashi-ku, Sapporo 007-0841, Japan 3 |
| Author_xml | – sequence: 1 givenname: Yuta surname: Shirogane fullname: Shirogane, Yuta organization: Department of Virology, Faculty of Medicine, Kyushu University, Fukuoka 812-8582 – sequence: 2 givenname: Makoto surname: Takeda fullname: Takeda, Makoto organization: Department of Virology, Faculty of Medicine, Kyushu University, Fukuoka 812-8582 – sequence: 3 givenname: Masaharu surname: Iwasaki fullname: Iwasaki, Masaharu organization: Department of Virology, Faculty of Medicine, Kyushu University, Fukuoka 812-8582 – sequence: 4 givenname: Nobuhisa surname: Ishiguro fullname: Ishiguro, Nobuhisa organization: Department of Pediatrics, Hokkaido University Graduate School of Medicine, Sapporo 060-8638 – sequence: 5 givenname: Hiroki surname: Takeuchi fullname: Takeuchi, Hiroki organization: Department of Virology, Faculty of Medicine, Kyushu University, Fukuoka 812-8582 – sequence: 6 givenname: Yuichiro surname: Nakatsu fullname: Nakatsu, Yuichiro organization: Department of Virology, Faculty of Medicine, Kyushu University, Fukuoka 812-8582 – sequence: 7 givenname: Maino surname: Tahara fullname: Tahara, Maino organization: Department of Virology, Faculty of Medicine, Kyushu University, Fukuoka 812-8582 – sequence: 8 givenname: Hideaki surname: Kikuta fullname: Kikuta, Hideaki organization: Pediatric Clinic, Touei Hospital, Kita-41, Higashi-6, Higashi-ku, Sapporo 007-0841, Japan – sequence: 9 givenname: Yusuke surname: Yanagi fullname: Yanagi, Yusuke organization: Department of Virology, Faculty of Medicine, Kyushu University, Fukuoka 812-8582 |
| BackLink | http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20599508$$DView record in Pascal Francis https://www.ncbi.nlm.nih.gov/pubmed/18562527$$D View this record in MEDLINE/PubMed |
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| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Corresponding author. Mailing address: Department of Virology, Faculty of Medicine, Kyushu University, Fukuoka 812-8582, Japan. Phone: 81-92-642-6138. Fax: 81-92-642-6140. E-mail: mtakeda@virology.med.kyushu-u.ac.jp |
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Mendeley... Human metapneumovirus (HMPV) is a major causative agent of severe bronchiolitis and pneumonia. Its fusion (F) protein must be cleaved by host proteases to... |
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| SubjectTerms | Animals Biological and medical sciences Cercopithecus aethiops Epithelium - metabolism Fundamental and applied biological sciences. Psychology Gene Expression Green Fluorescent Proteins - metabolism Lung - cytology Membrane Fusion Metapneumovirus - growth & development Microbiology Miscellaneous Serine Endopeptidases - genetics Serine Endopeptidases - metabolism Vero Cells Viral Fusion Proteins - metabolism Virology Virus-Cell Interactions |
| Title | Efficient Multiplication of Human Metapneumovirus in Vero Cells Expressing the Transmembrane Serine Protease TMPRSS2 |
| URI | http://jvi.asm.org/content/82/17/8942.abstract https://www.ncbi.nlm.nih.gov/pubmed/18562527 https://www.proquest.com/docview/69426193 https://pubmed.ncbi.nlm.nih.gov/PMC2519639 |
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