Rap-afadin axis in control of Rho signaling and endothelial barrier recovery

• Published in: Molecular biology of the cell Vol. 24; no. 17; pp. 2678 - 2688
• Main Authors: Birukova, Anna A, Tian, Xinyong, Tian, Yufeng, Higginbotham, Katherine, Birukov, Konstantin G
• Format: Journal Article
• Language: English
• Published: United States The American Society for Cell Biology 01.09.2013
• Subjects: Cytoskeleton - genetics; Cytoskeleton - metabolism; Down-Regulation; Endothelial Cells - cytology; Endothelial Cells - metabolism; Gene Knockdown Techniques; Guanine Nucleotide Exchange Factors - genetics; Guanine Nucleotide Exchange Factors - metabolism; Humans; Microfilament Proteins - metabolism; Phosphorylation; rap1 GTP-Binding Proteins - metabolism; rho GTP-Binding Proteins - genetics; rho GTP-Binding Proteins - metabolism; Signal Transduction - drug effects; Signal Transduction - genetics; Thrombin - pharmacology

Activation of the Rho GTPase pathway determines endothelial cell (EC) hyperpermeability after injurious stimuli. To date, feedback mechanisms of Rho down-regulation critical for barrier restoration remain poorly understood. We tested a hypothesis that Rho down-regulation and barrier recovery of agon...