Optimizing C-Type Natriuretic Peptide and Receptor Expression Analysis with Droplet Digital™ PCR: Advancing Biomarker Discovery for Brugada Syndrome?

• Published in: Biomolecules (Basel, Switzerland) Vol. 15; no. 6; p. 792
• Main Authors: Cabiati, Manuela, Vozzi, Federico, Persiani, Elisa, Piacenti, Marcello, Rossi, Andrea, Sgalippa, Agnese, Cecchettini, Antonella, Solarino, Gianluca, Zucchelli, Giulio, Mazzocchetti, Lorenzo, Notarstefano, Pasquale, Guiducci, Letizia, Morales, Maria Aurora, Del Ry, Silvia
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 29.05.2025; MDPI
• Subjects: Adult; Biomarkers; Biomarkers - blood; Blood; Body weight; Brugada syndrome; Brugada Syndrome - blood; Brugada Syndrome - diagnosis; Brugada Syndrome - genetics; Brugada Syndrome - metabolism; C-Type natriuretic peptide; Cardiac arrhythmia; Cardiology; Cardiovascular disease; Cardiovascular diseases; Case-Control Studies; Coronary artery disease; droplet digital™ PCR; Electrocardiogram; Electrocardiography; Female; Genes; Heart; Heart diseases; Humans; Inflammation; Kinases; Male; Middle Aged; mRNA; Mutation; Natriuretic Peptide, C-Type - blood; Natriuretic Peptide, C-Type - genetics; Natriuretic Peptide, C-Type - metabolism; Natriuretic peptides; NPR-B; NPR-C; Peptides; Physiology; Polymerase Chain Reaction - methods; Precision medicine; Public radio; Receptors, Atrial Natriuretic Factor - blood; Receptors, Atrial Natriuretic Factor - genetics; Receptors, Atrial Natriuretic Factor - metabolism; United States; Italy; California; mRNA; Brugada syndrome; C-type natriuretic peptide; NPR-B; NPR-C; droplet digital™ PCR

Brugada syndrome (BrS) is a cardiac disease associated with characteristic ECG abnormalities and a heightened risk of sudden cardiac death, especially in young individuals with structurally normal hearts. The primary aim of this study was to highlight, for the first time, the potential of using drop...