LC‐MS/MS isomeric profiling of permethylated N‐glycans derived from serum haptoglobin of hepatocellular carcinoma (HCC) and cirrhotic patients

• Published in: Electrophoresis Vol. 38; no. 17; pp. 2160 - 2167
• Main Authors: Huang, Yifan, Zhou, Shiyue, Zhu, Jianhui, Lubman, David M., Mechref, Yehia
• Format: Journal Article
• Language: English
• Published: Germany 01.09.2017
• Subjects: Biomarkers, Tumor - blood; Carcinoma, Hepatocellular - blood; Carcinoma, Hepatocellular - metabolism; Chromatography, Liquid - methods; Cirrhosis; Glycans biomarker discovery; Glycosylation; Haptoglobins - analysis; Haptoglobins - chemistry; Hepatocellular carcinoma; Humans; Isomeric separation; Isomerism; LC‐MS/MS; Liver Cirrhosis - blood; Liver Cirrhosis - metabolism; Liver Neoplasms - blood; Liver Neoplasms - metabolism; Permethylated glycans; Polysaccharides - analysis; Polysaccharides - chemistry; Principal Component Analysis; Tandem Mass Spectrometry - methods; Cirrhosis; LC-MS/MS; Hepatocellular carcinoma; Glycans biomarker discovery; Permethylated glycans; Isomeric separation

Early stage detection and cancer treatment demand the identification of reliable biomarkers. Over the past decades, efforts have been devoted to assess the variation of glycosylation level as well as the glycan structures of proteins in blood or serum, associated with the development and/or progress...