Strategies for Site‐Specific Labeling of Receptor Proteins on the Surfaces of Living Cells by Using Genetically Encoded Peptide Tags

• Published in: Chembiochem : a European journal of chemical biology Vol. 22; no. 10; pp. 1717 - 1732
• Main Authors: Wolf, Philipp, Gavins, Georgina, Beck‐Sickinger, Annette G., Seitz, Oliver
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 14.05.2021; John Wiley and Sons Inc
• Subjects: Animals; bioorganic chemistry; Cell surface; Cellular communication; Coils; Complementation; Coordination compounds; Fluorescence; Fluorescence microscopy; Fluorescence Resonance Energy Transfer; Fluorescent Dyes - chemistry; Genetic code; Humans; Kinases; Labeling; Luminescent Proteins - chemistry; Luminescent Proteins - metabolism; membrane proteins; Metal complexes; Microscopy, Fluorescence; Peptides; Peptides - chemistry; Peptides - metabolism; Perturbation; protein modification; Proteins; Receptor Protein-Tyrosine Kinases - chemistry; Receptor Protein-Tyrosine Kinases - metabolism; Receptors; Receptors, G-Protein-Coupled - chemistry; Receptors, G-Protein-Coupled - metabolism; Review; Reviews; signal transduction; Staining and Labeling - methods; Tags; Tyrosine; protein modification; membrane proteins; signal transduction; fluorescence microscopy; bioorganic chemistry

Fluorescence microscopy imaging enables receptor proteins to be investigated within their biological context. A key challenge is to site‐specifically incorporate reporter moieties into proteins without interfering with biological functions or cellular networks. Small peptide tags offer the opportuni...