Singlet‐Oxygen Generation by Peroxidases and Peroxygenases for Chemoenzymatic Synthesis

• Published in: Chembiochem : a European journal of chemical biology Vol. 22; no. 2; pp. 398 - 407
• Main Authors: Ingenbosch, Kim N., Quint, Stephan, Dyllick‐Brenzinger, Melanie, Wunschik, Dennis S., Kiebist, Jan, Süss, Philipp, Liebelt, Ute, Zuhse, Ralf, Menyes, Ulf, Scheibner, Katrin, Mayer, Christian, Opwis, Klaus, Gutmann, Jochen S., Hoffmann‐Jacobsen, Kerstin
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 15.01.2021; John Wiley and Sons Inc
• Subjects: Bleaching; Catalase; Chemical synthesis; chemo-enzymatic synthesis; Decoloring; Fluorescence; Fluorescent Dyes - chemistry; Mixed Function Oxygenases - chemistry; Mixed Function Oxygenases - metabolism; Molecular Structure; Oxygen; Oxygen probes; Peroxidase; Peroxidases - chemistry; Peroxidases - metabolism; peroxygenase; Reactive oxygen species; Reagents; Singlet oxygen; Singlet Oxygen - chemistry; Singlet Oxygen - metabolism; singlet oxygen sensor green; Substrate inhibition; chemo-enzymatic synthesis; singlet oxygen; fluorescence; peroxidase; singlet oxygen sensor green; peroxygenase

Singlet oxygen is a reactive oxygen species undesired in living cells but a rare and valuable reagent in chemical synthesis. We present a fluorescence spectroscopic analysis of the singlet‐oxygen formation activity of commercial peroxidases and novel peroxygenases. Singlet‐oxygen sensor green (SOSG)...