Characterization of antifungal metabolite produced by a new strain Pseudomonas aeruginosa PUPa3 that exhibits broad‐spectrum antifungal activity and biofertilizing traits

• Published in: Journal of applied microbiology Vol. 98; no. 1; pp. 145 - 154
• Main Authors: Kumar, R. Sunish, Ayyadurai, N., Pandiaraja, P., Reddy, A.V., Venkateswarlu, Y., Prakash, O., Sakthivel, N.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 01.01.2005; Blackwell Science; Oxford University Press
• Subjects: Anti-Bacterial Agents; antifungal activity; Antifungal Agents - chemistry; Antifungal Agents - isolation & purification; Biological and medical sciences; Fertilizers; Food Microbiology; Fundamental and applied biological sciences. Psychology; indole‐3‐acetic acid; Magnetic Resonance Spectroscopy; mass spectroscopy; Microbiology; nuclear magnetic resonance; Oryza; Oryza sativa; phenazine‐1‐carboxamide; Pseudomonas aeruginosa; Pseudomonas aeruginosa - genetics; Pseudomonas aeruginosa - metabolism; RNA, Ribosomal, 18S - analysis; Pseudomonadales; Applied microbiology; Metabolite; Indole; phenazine-1- carboxamide; NMR spectrometry; antifungal activity; mass spectroscopy; nuclear magnetic resonance; Characterization; Antifungal agent; Bacteria; Pseudomonadaceae; Pseudomonas aeruginosa; Activity spectrum; Acetic acid; indole-3-acetic acid
• ISSN: 1364-5072; 1365-2672
• DOI: 10.1111/j.1365-2672.2004.02435.x

Aim:  To study the antifungal activity and plant beneficial traits of a broad‐spectrum antagonistic fluorescent pseudomonad strain, PUPa3. Methods and Results:  Strain PUPa3 was isolated from the rhizosphere soil of rice and identified as Pseudomonas aeruginosa on the basis of biochemical tests and by comparison of 16S rDNA sequences. This bacterium exhibits a broad‐spectrum antifungal activity towards phytopathogenic fungi. The antifungal metabolite by PUPa3 was extracted, purified and characterized using nuclear magnetic resonance (NMR) and mass spectroscopy (MS). Production of indole‐3‐acetic acid (IAA), siderophores, phosphatase and protease in PUPa3 was determined. Strain PUPa3 did not produce hydrogen cyanide, cellulase and pectinase. Conclusion:  The antifungal metabolite produced by PUPa3 has been identified as phenazine‐1‐carboxamide (PCN) on the basis of NMR and MS data. Strain PUPa3 showed a broad‐spectrum antifungal activity towards a range of phytopathogenic fungi. This bacterium also showed several plant growth‐promoting traits but did not show the traits attributed to deleterious rhizobacteria. Significance and Impact of the Study:  Present study reports the production of PCN as well as IAA for the first time by a saprophytic P. aeruginosa strain PUPa3. Because of the production of siderophore, growth hormone, protease and phosphatase and its innate fungicidal potential, this strain can be used as biofertilizer and antagonist against a range of phytopathogenic fungi that infect rice, groundnut, tobacco, chili, mango, sugarcane, tea, cotton and banana.