Crystallization and preliminary X-ray studies of ferric uptake regulator from Magnetospirillum gryphiswaldense
Magnetosomes in magnetotactic bacteria have been widely used in studies of magnetic domains and in commercial applications. The iron content of magnetotactic bacteria is ∼100 times higher than that of Escherichia coli. Magnetospirillum gryphiswaldense MSR‐1 can still take up iron even at high intrac...
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Published in | Acta crystallographica. Section F, Structural biology and crystallization communications Vol. 68; no. 8; pp. 902 - 905 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
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5 Abbey Square, Chester, Cheshire CH1 2HU, England
International Union of Crystallography
01.08.2012
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Abstract | Magnetosomes in magnetotactic bacteria have been widely used in studies of magnetic domains and in commercial applications. The iron content of magnetotactic bacteria is ∼100 times higher than that of Escherichia coli. Magnetospirillum gryphiswaldense MSR‐1 can still take up iron even at high intracellular concentrations. Ferric uptake regulator (Fur) is a global iron‐responsive regulator that affects magnetosome formation, iron transport and oxygen metabolism. However, the mechanism of iron uptake and homeostasis by M. gryphiswaldense MSR‐1 Fur is not clear. Here, the expression, purification and crystallization of apo and SeMet Fur from M. gryphiswaldense MSR‐1 are reported. The crystals belonged to space group C2. Matthews coefficient analysis and size‐exclusion chromatography showed that the asymmetric unit probably contains one dimer of Fur. Diffraction data were optimized to 1.58 Å resolution for apo Fur and to 1.9 Å resolution for SeMet Fur. |
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AbstractList | Magnetosomes in magnetotactic bacteria have been widely used in studies of magnetic domains and in commercial applications. The iron content of magnetotactic bacteria is ∼100 times higher than that of Escherichia coli. Magnetospirillum gryphiswaldense MSR-1 can still take up iron even at high intracellular concentrations. Ferric uptake regulator (Fur) is a global iron-responsive regulator that affects magnetosome formation, iron transport and oxygen metabolism. However, the mechanism of iron uptake and homeostasis by M. gryphiswaldense MSR-1 Fur is not clear. Here, the expression, purification and crystallization of apo and SeMet Fur from M. gryphiswaldense MSR-1 are reported. The crystals belonged to space group C2. Matthews coefficient analysis and size-exclusion chromatography showed that the asymmetric unit probably contains one dimer of Fur. Diffraction data were optimized to 1.58 Å resolution for apo Fur and to 1.9 Å resolution for SeMet Fur. Crystals of apo and SeMet ferric uptake regulator from the magnetotactic bacterium M. gryphiswaldense MSR-1 were obtained and optimized to obtain high-quality diffraction data. Diffraction data sets were collected and processed at 1.58 and 1.9 Å resolution, respectively. Magnetosomes in magnetotactic bacteria have been widely used in studies of magnetic domains and in commercial applications. The iron content of magnetotactic bacteria is ∼100 times higher than that of Escherichia coli . Magnetospirillum gryphiswaldense MSR-1 can still take up iron even at high intracellular concentrations. Ferric uptake regulator (Fur) is a global iron-responsive regulator that affects magnetosome formation, iron transport and oxygen metabolism. However, the mechanism of iron uptake and homeostasis by M. gryphiswaldense MSR-1 Fur is not clear. Here, the expression, purification and crystallization of apo and SeMet Fur from M. gryphiswaldense MSR-1 are reported. The crystals belonged to space group C 2. Matthews coefficient analysis and size-exclusion chromatography showed that the asymmetric unit probably contains one dimer of Fur. Diffraction data were optimized to 1.58 Å resolution for apo Fur and to 1.9 Å resolution for SeMet Fur. Magnetosomes in magnetotactic bacteria have been widely used in studies of magnetic domains and in commercial applications. The iron content of magnetotactic bacteria is similar to 100 times higher than that of Escherichia coli. Magnetospirillum gryphiswaldense MSR-1 can still take up iron even at high intracellular concentrations. Ferric uptake regulator (Fur) is a global iron-responsive regulator that affects magnetosome formation, iron transport and oxygen metabolism. However, the mechanism of iron uptake and homeostasis by M. gryphiswaldense MSR-1 Fur is not clear. Here, the expression, purification and crystallization of apo and SeMet Fur from M. gryphiswaldense MSR-1 are reported. The crystals belonged to space group C2. Matthews coefficient analysis and size-exclusion chromatography showed that the asymmetric unit probably contains one dimer of Fur. Diffraction data were optimized to 1.58Aa resolution for apo Fur and to 1.9Aa resolution for SeMet Fur. |
Author | Liu, Zhao Wu, Wei Chen, Zhongzhou |
Author_xml | – sequence: 1 givenname: Zhao surname: Liu fullname: Liu, Zhao organization: State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, People's Republic of China – sequence: 2 givenname: Zhongzhou surname: Chen fullname: Chen, Zhongzhou organization: State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, People's Republic of China – sequence: 3 givenname: Wei surname: Wu fullname: Wu, Wei organization: State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, People's Republic of China |
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References | Sheikh (fw5365_bb14) 2009; 72 Studier (fw5365_bb15) 2005; 41 Winn (fw5365_bb20) 2011; 67 Qi (fw5365_bb10) 2012; 7 Blakemore (fw5365_bb3) 1975; 190 Scheffel (fw5365_bb12) 2006; 440 Otwinowski (fw5365_bb8) 1997; 276 Trapani (fw5365_bb16) 2008; 64 Frankel (fw5365_bb5) 1979; 203 Hantke (fw5365_bb6) 2001; 4 Vonrhein (fw5365_bb19) 2007; 364 Rangarajan (fw5365_bb11) 2010; 66 Pohl (fw5365_bb9) 2003; 47 Evans (fw5365_bb4) 2001; 34 McCoy (fw5365_bb7) 2007; 63 Uebe (fw5365_bb17) 2011; 192 Bazylinski (fw5365_bb2) 2004; 2 Altschul (fw5365_bb1) 1990; 215 Schultheiss (fw5365_bb13) 2003; 179 Vagin (fw5365_bb18) 2010; 66 |
References_xml | – volume: 203 start-page: 1355 year: 1979 ident: fw5365_bb5 publication-title: Science doi: 10.1126/science.203.4387.1355 contributor: fullname: Frankel – volume: 34 start-page: 82 year: 2001 ident: fw5365_bb4 publication-title: J. Appl. Cryst. doi: 10.1107/S0021889800014655 contributor: fullname: Evans – volume: 2 start-page: 217 year: 2004 ident: fw5365_bb2 publication-title: Nature Rev. Microbiol. doi: 10.1038/nrmicro842 contributor: fullname: Bazylinski – volume: 215 start-page: 403 year: 1990 ident: fw5365_bb1 publication-title: J. Mol. Biol. doi: 10.1016/S0022-2836(05)80360-2 contributor: fullname: Altschul – volume: 190 start-page: 377 year: 1975 ident: fw5365_bb3 publication-title: Science doi: 10.1126/science.170679 contributor: fullname: Blakemore – volume: 66 start-page: 1617 year: 2010 ident: fw5365_bb11 publication-title: Acta Cryst. F doi: 10.1107/S1744309110041059 contributor: fullname: Rangarajan – volume: 47 start-page: 903 year: 2003 ident: fw5365_bb9 publication-title: Mol. Microbiol. doi: 10.1046/j.1365-2958.2003.03337.x contributor: fullname: Pohl – volume: 72 start-page: 1208 year: 2009 ident: fw5365_bb14 publication-title: Mol. Microbiol. doi: 10.1111/j.1365-2958.2009.06718.x contributor: fullname: Sheikh – volume: 276 start-page: 307 year: 1997 ident: fw5365_bb8 publication-title: Methods Enzymol. doi: 10.1016/S0076-6879(97)76066-X contributor: fullname: Otwinowski – volume: 179 start-page: 89 year: 2003 ident: fw5365_bb13 publication-title: Arch. Microbiol. doi: 10.1007/s00203-002-0498-z contributor: fullname: Schultheiss – volume: 41 start-page: 207 year: 2005 ident: fw5365_bb15 publication-title: Protein Expr. Purif. doi: 10.1016/j.pep.2005.01.016 contributor: fullname: Studier – volume: 364 start-page: 215 year: 2007 ident: fw5365_bb19 publication-title: Methods Mol. Biol. contributor: fullname: Vonrhein – volume: 440 start-page: 110 year: 2006 ident: fw5365_bb12 publication-title: Nature (London) doi: 10.1038/nature04382 contributor: fullname: Scheffel – volume: 64 start-page: 11 year: 2008 ident: fw5365_bb16 publication-title: Acta Cryst. D doi: 10.1107/S0907444907044460 contributor: fullname: Trapani – volume: 4 start-page: 172 year: 2001 ident: fw5365_bb6 publication-title: Curr. Opin. Microbiol. doi: 10.1016/S1369-5274(00)00184-3 contributor: fullname: Hantke – volume: 7 start-page: e29572 year: 2012 ident: fw5365_bb10 publication-title: PLoS One doi: 10.1371/journal.pone.0029572 contributor: fullname: Qi – volume: 63 start-page: 32 year: 2007 ident: fw5365_bb7 publication-title: Acta Cryst. D doi: 10.1107/S0907444906045975 contributor: fullname: McCoy – volume: 67 start-page: 235 year: 2011 ident: fw5365_bb20 publication-title: Acta Cryst. D doi: 10.1107/S0907444910045749 contributor: fullname: Winn – volume: 192 start-page: 4192 year: 2011 ident: fw5365_bb17 publication-title: J. Bacteriol. doi: 10.1128/JB.00319-10 contributor: fullname: Uebe – volume: 66 start-page: 22 year: 2010 ident: fw5365_bb18 publication-title: Acta Cryst. D doi: 10.1107/S0907444909042589 contributor: fullname: Vagin |
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Snippet | Magnetosomes in magnetotactic bacteria have been widely used in studies of magnetic domains and in commercial applications. The iron content of magnetotactic... Crystals of apo and SeMet ferric uptake regulator from the magnetotactic bacterium M. gryphiswaldense MSR-1 were obtained and optimized to obtain high-quality... |
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SubjectTerms | Bacteria Bacterial Proteins - chemistry Coefficients Crystallization Crystallization Communications Crystallography, X-Ray Diffraction ferric uptake regulator Homeostasis Iron Magnetospirillum - chemistry Magnetospirillum gryphiswaldense MSR-1 magnetotactic bacteria Regulators Repressor Proteins - chemistry Uptakes |
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Title | Crystallization and preliminary X-ray studies of ferric uptake regulator from Magnetospirillum gryphiswaldense |
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