A proteomic analysis of penicillin resistance in Streptococcus pneumoniae reveals a novel role for PstS, a subunit of the phosphate ABC transporter

• Published in: Molecular microbiology Vol. 58; no. 5; pp. 1430 - 1440
• Main Authors: Soualhine, Hafid, Brochu, Vicky, Ménard, François, Papadopoulou, Barbara, Weiss, Karl, Bergeron, Michel G., Légaré, Danielle, Drummelsmith, Jolyne, Ouellette, Marc
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 01.12.2005; Blackwell Science; Blackwell Publishing Ltd
• Subjects: Analytical, structural and metabolic biochemistry; Anti-Bacterial Agents - pharmacology; ATP-Binding Cassette Transporters - chemistry; ATP-Binding Cassette Transporters - genetics; ATP-Binding Cassette Transporters - metabolism; Bacteria; Bacterial Proteins - chemistry; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Bacteriology; Biological and medical sciences; Drug resistance; Fundamental and applied biological sciences. Psychology; Gene expression; Gene Expression Regulation, Bacterial; Humans; Microbial Sensitivity Tests; Microbiology; Miscellaneous; Pathogens; Penicillin; Penicillin Resistance; Penicillins - pharmacology; Phosphate Transport Proteins - chemistry; Phosphate Transport Proteins - genetics; Phosphate Transport Proteins - metabolism; Proteins; Proteome; Streptococcus pneumoniae; Streptococcus pneumoniae - drug effects; Streptococcus pneumoniae - genetics; Microbiology; Subunit; Streptococcus pneumoniae; Resistance; Streptococcaceae; Antibiotic; Proteomics; Penicillin; Streptococcus A; Bacteria; Micrococcales; Antibacterial agent; ABC transporter

Summary Resistance to penicillin is widespread in the Gram‐positive bacterium Streptococcus pneumoniae, and while several mutations are known to be implicated in resistance other mechanisms are likely to occur. We used a proteomic screen of two independent mutants in which resistance was selected in...