Serum miRNAs panel (miR-16-2, miR-195, miR-2861, miR-497) as novel non-invasive biomarkers for detection of cervical cancer

• Published in: Scientific reports Vol. 5; no. 1; p. 17942
• Main Authors: Zhang, Yujuan, Zhang, Donghong, Wang, Fei, Xu, Danfei, Guo, Ye, Cui, Wei
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 14.12.2015; Nature Publishing Group
• Subjects: 13/109; 13/2; 49/47; 64/60; 692/53/2421; 692/699/67/1857; Adult; Animals; Biomarkers, Tumor; Breast cancer; Cervical cancer; Cervix; Disease Models, Animal; Early Detection of Cancer; Female; Gene Expression Profiling; Genes, Tumor Suppressor; Human papillomavirus; Humanities and Social Sciences; Humans; MicroRNAs - blood; MicroRNAs - genetics; Middle Aged; miRNA; multidisciplinary; Neoplasm Grading; Neoplasm Invasiveness; Neoplasm Staging; Ovarian cancer; Ovarian Neoplasms - genetics; Polymerase chain reaction; Prognosis; Science; Sensitivity and Specificity; Transcription; Tumorigenesis; Uterine Cervical Neoplasms - blood; Uterine Cervical Neoplasms - diagnosis; Uterine Cervical Neoplasms - genetics
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep17942

miRNAs have been established as critical layer of regulation during tumorigenesis; extracellular miRNAs are extraordinarily stable; and, quantitative reverse transcript polymerase chain reaction (qRT-PCR) provides a sensitive platform for quantifying miRNAs with a broad dynamic range. Herein, we aimed to establish a serum miRNA signature for diagnosing cervical cancer (CC). In this study, we recruited a cohort of 184 CC, 186 cervical intraepithelial neoplasia (CIN) patients and 193 healthy control subjects. qRT-PCR was performed with serum samples to screen a pool of 444 miRNAs at the initial phase, 66 miRNAs at the training phase and 7 miRNAs at the validation phase. The profile of 4 circulating miRNAs (miR-16-2*, miR-195, miR-2861, miR-497) was established for CC diagnosis. By Receiver Operating Characteristic (ROC) curve analysis, this 4-miRNA signature showed high accuracy in discriminating CC (AUC = 0.849) and CIN individuals (AUC = 0.734) from healthy controls. Among these 4 miRNAs, only miR-16-2*, but not miR-195, miR-2861 or miR497, shared a similar pattern in sera of breast cancer and ovarian cancer patients. Overall, our studies have identified a novel noninvasive biomarker constituted with a panel of four miRNAs (miR-16-2*, miR-195, miR-2861, miR-497).