Amino acids substitutions in the PB2 protein of H7N9 influenza A viruses are important for virulence in mammalian hosts

We tested the biological significance of two amino acid mutations in the PB2 protein (glutamic acid to lysine at position 627 and aspartic acid to asparagine at position 701) of A(H7N9) viruses for mammalian adaptation. Mutants were assessed for their viral polymerase activities, growth kinetics in...

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Published inScientific reports Vol. 5; no. 1; p. 8039
Main Authors Yamayoshi, Seiya, Fukuyama, Satoshi, Yamada, Shinya, Zhao, Dongming, Murakami, Shin, Uraki, Ryuta, Watanabe, Tokiko, Tomita, Yuriko, Neumann, Gabriele, Kawaoka, Yoshihiro
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 27.01.2015
Nature Publishing Group
Subjects
631/326/596
631/326/596/1578
Adaptation
Amino Acid Substitution
Amino acids
Animals
Asparagine
Aspartic acid
Avian cells
Body Weight
Cell Line
Dogs
Female
Genes, Reporter
Glutamic acid
Growth kinetics
Humanities and Social Sciences
Humans
Infections
Influenza
Influenza A
Influenza A Virus, H7N9 Subtype - metabolism
Influenza A Virus, H7N9 Subtype - pathogenicity
Lung - virology
Lysine
Madin Darby Canine Kidney Cells
Mammals
Mice
Mice, Inbred BALB C
multidisciplinary
Mutation
Nasal Cavity - virology
Pathogenicity
Pathogens
Plasmids
Proteins
Science
Viral Proteins - genetics
Virology
Virulence
Virulence - genetics
Virus Replication
Viruses
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Abstract We tested the biological significance of two amino acid mutations in the PB2 protein (glutamic acid to lysine at position 627 and aspartic acid to asparagine at position 701) of A(H7N9) viruses for mammalian adaptation. Mutants were assessed for their viral polymerase activities, growth kinetics in mammalian and avian cells and pathogenicity in mice. We found that lysine at position 627 and asparagine at position 701 in PB2 are essential for mammalian adaptation of A(H7N9) viruses.
AbstractList We tested the biological significance of two amino acid mutations in the PB2 protein (glutamic acid to lysine at position 627 and aspartic acid to asparagine at position 701) of A(H7N9) viruses for mammalian adaptation. Mutants were assessed for their viral polymerase activities, growth kinetics in mammalian and avian cells and pathogenicity in mice. We found that lysine at position 627 and asparagine at position 701 in PB2 are essential for mammalian adaptation of A(H7N9) viruses.
We tested the biological significance of two amino acid mutations in the PB2 protein (glutamic acid to lysine at position 627 and aspartic acid to asparagine at position 701) of A(H7N9) viruses for mammalian adaptation. Mutants were assessed for their viral polymerase activities, growth kinetics in mammalian and avian cells, and pathogenicity in mice. We found that lysine at position 627 and asparagine at position 701 in PB2 are essential for mammalian adaptation of A(H7N9) viruses.We tested the biological significance of two amino acid mutations in the PB2 protein (glutamic acid to lysine at position 627 and aspartic acid to asparagine at position 701) of A(H7N9) viruses for mammalian adaptation. Mutants were assessed for their viral polymerase activities, growth kinetics in mammalian and avian cells, and pathogenicity in mice. We found that lysine at position 627 and asparagine at position 701 in PB2 are essential for mammalian adaptation of A(H7N9) viruses.
ArticleNumber 8039
Author Uraki, Ryuta
Tomita, Yuriko
Kawaoka, Yoshihiro
Neumann, Gabriele
Zhao, Dongming
Yamayoshi, Seiya
Watanabe, Tokiko
Yamada, Shinya
Fukuyama, Satoshi
Murakami, Shin
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  fullname: Fukuyama, Satoshi
  organization: Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, ERATO Infection-Induced Host Responses Project, Japan Science and Technology Agency
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  givenname: Shinya
  surname: Yamada
  fullname: Yamada, Shinya
  organization: Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo
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  givenname: Dongming
  surname: Zhao
  fullname: Zhao, Dongming
  organization: Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, ERATO Infection-Induced Host Responses Project, Japan Science and Technology Agency
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  givenname: Yuriko
  surname: Tomita
  fullname: Tomita, Yuriko
  organization: Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, ERATO Infection-Induced Host Responses Project, Japan Science and Technology Agency
– sequence: 9
  givenname: Gabriele
  surname: Neumann
  fullname: Neumann, Gabriele
  organization: Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison
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  givenname: Yoshihiro
  surname: Kawaoka
  fullname: Kawaoka, Yoshihiro
  organization: Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, ERATO Infection-Induced Host Responses Project, Japan Science and Technology Agency, Department of Special Pathogens, International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison
BackLink https://www.ncbi.nlm.nih.gov/pubmed/25623817$$D View this record in MEDLINE/PubMed
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Snippet We tested the biological significance of two amino acid mutations in the PB2 protein (glutamic acid to lysine at position 627 and aspartic acid to asparagine...
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SubjectTerms 631/326/596
631/326/596/1578
Adaptation
Amino Acid Substitution
Amino acids
Animals
Asparagine
Aspartic acid
Avian cells
Body Weight
Cell Line
Dogs
Female
Genes, Reporter
Glutamic acid
Growth kinetics
Humanities and Social Sciences
Humans
Infections
Influenza
Influenza A
Influenza A Virus, H7N9 Subtype - metabolism
Influenza A Virus, H7N9 Subtype - pathogenicity
Lung - virology
Lysine
Madin Darby Canine Kidney Cells
Mammals
Mice
Mice, Inbred BALB C
multidisciplinary
Mutation
Nasal Cavity - virology
Pathogenicity
Pathogens
Plasmids
Proteins
Science
Viral Proteins - genetics
Virology
Virulence
Virulence - genetics
Virus Replication
Viruses
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Title Amino acids substitutions in the PB2 protein of H7N9 influenza A viruses are important for virulence in mammalian hosts
URI https://link.springer.com/article/10.1038/srep08039
https://www.ncbi.nlm.nih.gov/pubmed/25623817
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Volume 5
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