Oligonucleotide Probes for ND-FISH Analysis to Identify Rye and Wheat Chromosomes

• Published in: Scientific reports Vol. 5; no. 1; p. 10552
• Main Authors: Fu, Shulan, Chen, Lei, Wang, Yangyang, Li, Meng, Yang, Zujun, Qiu, Ling, Yan, Benju, Ren, Zhenglong, Tang, Zongxiang
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 21.05.2015; Nature Publishing Group
• Subjects: 13; 14; 38/32; 631/1647/1407/1405; 631/449/711; Chromosomes; Chromosomes, Plant - genetics; Chromosomes, Plant - metabolism; Deoxyribonucleic acid; DNA; DNA probes; Fluorescence in situ hybridization; Fluorescent indicators; Genomic in situ hybridization; Humanities and Social Sciences; Hybrids; In Situ Hybridization, Fluorescence; Microscopy, Fluorescence; multidisciplinary; Oligonucleotide Probes - genetics; Oligonucleotide Probes - metabolism; Oligonucleotides; Polyploidy; Science; Secale - genetics; Triticum - genetics; Triticum aestivum

Genomic in situ hybridization (GISH) has been widely used to detect rye ( Secale cereale L.) chromosomes in wheat ( Triticum aestivum L.) introgression lines. The routine procedure of GISH using genomic DNA of rye as a probe is time-consuming and labor-intensive because of the preparation and labeli...