Culture-independent method for identification of microbial enzyme-encoding genes by activity-based single-cell sequencing using a water-in-oil microdroplet platform

• Published in: Scientific reports Vol. 6; no. 1; p. 22259
• Main Authors: Nakamura, Kazuki, Iizuka, Ryo, Nishi, Shinro, Yoshida, Takao, Hatada, Yuji, Takaki, Yoshihiro, Iguchi, Ayaka, Yoon, Dong Hyun, Sekiguchi, Tetsushi, Shoji, Shuichi, Funatsu, Takashi
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 26.02.2016; Nature Publishing Group
• Subjects: 14/34; 14/63; 38/23; 631/1647/2217/2220; 631/1647/2234; Bacteria - classification; Bacteria - cytology; Bacteria - genetics; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Bacteriological Techniques; beta-Glucosidase - genetics; beta-Glucosidase - metabolism; Cell culture; Enzymatic activity; Enzymes; Fluorescent Dyes - metabolism; Genetic resources; Genomes; Humanities and Social Sciences; Kinetics; Microscopy, Fluorescence; multidisciplinary; Oils - chemistry; Reproducibility of Results; RNA, Ribosomal, 16S - genetics; Science; Seawater - microbiology; Sequence Analysis, DNA - methods; Single-Cell Analysis - instrumentation; Single-Cell Analysis - methods; Water - chemistry; Water Microbiology - standards

Environmental microbes are a great source of industrially valuable enzymes with potent and unique catalytic activities. Unfortunately, the majority of microbes remain unculturable and thus are not accessible by culture-based methods. Recently, culture-independent metagenomic approaches have been suc...